A Universal and Ultrastable Mineralization Coating Bioinspired from Biofilms

Abstract

A simple and universal method for manufacturing a mineralization coating on various surfaces is developed using a biofilm-based material obtained from engineered curli nanofibers. The amyloid protein (CsgA) is the main proteinaceous component in the Escherichia coli (E. coli) biofilm, which can withstand detergents in the harsh environment. The peptide sequence DDDEEK is bioinspired from salivary acquired pellicles in the dental plaque biofilm, having a strong ability to absorb mineral ions and induce the formation of biominerals. The bioinspired coating is successfully secreted by the engineered E. coli, which is transformed with a recombinant plasmid for expression with T7 promoter (PET), namely PET-22b-CsgA-DDDEEK plasmid. The uniform coating can bear shear force and stay on virtually any type of material surface for at least one month. Moreover, the coated slices had a good mineralization performance and better stability than hydroxyapatite (HA)-spray slices. Furthermore, MG63 cells on the bioactive HA layer induced by the coating possess a better growth capacity than those on the commercial product Matrigel. The animal experiment results suggest that the coated Ti 6 Al 4 V screws with induced HA present better osteogenicity and osseointegration than HAsprayed screws after 12 weeks, as well as no extra immunogenicity. Thus, the coating is highly promising for biomedical applications.

3D Printing of Bioinspired Biomaterials for Tissue Regeneration

“…[66,67] Recently bioinspired mineralization has been applied in multiple biomaterials to enhance the bioactivity of materials system. [68][69][70][71] For example, graphene, as a 2D material has been considered as a potential biomaterial in tissue regeneration due to its large surface area, brilliant mechanical properties, and potential to modulate the biological performance. [70,72] For further improving the bioactivity of graphene, we proposed to introduce the inorganic calcium silicate into the multilayered graphene oxide/chitosan/calcium silicate biomaterials to repair the bone defect through vacuum filtration self-assembly method (Figure 3a,b).…”

Advances in refunctionalization of erythrocyte-based nanomedicine for enhancing cancer-targeted drug delivery

“…For instance, a tumor-targeting peptide c(RGDyK) could be attached to the RBC-coated drug nanocrystals via biotin-avidin bridges followed by a lipid insertion method for targeting glioma and delivering the chemotherapeutic drugs 54. Interestingly, this one of the strongest known non-covalent interaction in nature allows bioengineered motile bacteria, Escherichia coli MG1655, to be conjugated with RBCs to construct bacteria-driven microswimmers for cancer drug delivery and PTT 55, 56.…”

Atherosclerosis Treatment with Stimuli‐Responsive Nanoagents: Recent Advances and Future Perspectives

“…To synergistically combine their advantages, organic and inorganic nanoparticles can be incorporated in nanoassemblies, referred to as hybrid nanoparticles . Various methods, such as polycarbonate membrane extrusion, sonication, dialysis, microemulsion, self‐assembly, and sol‐gel techniques, can be used to synthesize nanoparticles . The ultimate aims of nanoparticle design are for (i) targeting, (ii) controlled drug release, (iii) enhanced imaging, and (iv) theranostics.…”