A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa.

Bowyer, Sheila Mary; Staden, L. Van; Kew, Michael C.; Sim, John

doi:10.1099/0022-1317-78-7-1719

Cited by 158 publications

(179 citation statements)

References 23 publications

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“…As for genotype A, there are two subgenotypes with different geographical distributions. Subgenotype Ae is common in Europe and the United States, while Aa is prevalent in Asia and Africa [Bowyer et al, 1997;Sugauchi et al, 2004]. In a casecontrol study, HBeAg was more frequent and HBV DNA levels higher in carriers of Ae than Aa .…”

Section: Discussionmentioning

confidence: 96%

“…They had the median serum HBV DNA level at 4.2 log copies/ml, and HBeAg was detected in sera from 32 (37%). Subgenotypes of A [Bowyer et al, 1997;Sugauchi et al, 2004] were Aa (Asian or African type) in 5 (6%) and Ae (European type) in 65 (75%); they were not classifiable in the remaining 17 (19%).…”

Section: Patients Chronically Infected With Hbvmentioning

confidence: 99%

“…Subgenotypes of A designated Ae prevalent in Europe and Aa frequent in Africa as well as Asia (corresponding to A 0 originally reported by Bowyer et al [1997]), were determined by the nucleotide sequence in the S gene . Briefly, nucleic acids were extracted from serum and a sequence of the large S gene was amplified by PCR with nested primers.…”

Section: Genotypes Of Hbvmentioning

confidence: 99%

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Virological outcomes in patients infected chronically with hepatitis B virus genotype A in comparison with genotypes B and C

Kobayashi

Akuta

Suzuki

et al. 2005

Journal of Medical Virology

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In a single hospital in Tokyo, the 87 patients infected persistently with hepatitis B virus (HBV) genotype A, the 413 with B, and the 3,389 with C were compared for virological outcome. Hepatitis B surface antigen (HBsAg) was cleared from the serum in 12% (3/26), 2% (2/112), and 3% (23/826) of patients with genotypes A, B, and C, respectively, at 5 years of follow-up (P ¼ 0.0395). Hepatitis B e antigen (HBeAg) was cleared from serum more frequently in patients with genotype B than those with A or C (78% [32/41] vs. 58% [11/19]

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Section: Discussionmentioning

confidence: 96%

Section: Patients Chronically Infected With Hbvmentioning

confidence: 99%

Section: Genotypes Of Hbvmentioning

confidence: 99%

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Virological outcomes in patients infected chronically with hepatitis B virus genotype A in comparison with genotypes B and C

Kobayashi

Akuta

Suzuki

et al. 2005

Journal of Medical Virology

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“…In many countries where well known waves of migration have occurred over time, the prevalence of different HBV genotypes reflects the origin of immigrants and other patterns of migration. This is exemplified by South Africa, where the most prevalent genotypes, A and D [7], correlate with migration from Northwestern Europe (UK and Netherlands), Southern Europe and India. The same explanation holds for the predominance of genotypes A and D in our population.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Hepatitis B Virus Genotypes and Its Association with Severity of Liver Disease in Patients with Chronic Hepatitis B in Uttar Pradesh, India

et al. 2011

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The present study was designed to investigate the distribution of genotypes and its association with severity of liver disease in patients with Chronic Hepatitis B (CHB) in Uttar Pradesh, India. One hundred five HBsAg positive patients were selected for the study. The DNA was extracted by using pure viral DNA extraction kit. The genotype of Hepatitis B virus (HBV) was identified by using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method, by using AvaII and DpnII restriction enzyme to see the different patterns of cleavage that would occur at this specific site. Among 105 HBsAg positive chronic liver disease patients 58 patients were positive for HBeAg and 47 samples were HBeAg negative. Genotyping was done successfully in 91 samples. Genotype A was identified in 22% and genotype D in 78% CHB patients. Genotype A showed elevated liver enzymes much more than genotype D. The Child Pugh classification of 20 patients with genotype A was class A (n = 2), B (n = 9), C (n = 9) and of genotype D was class A (n = 13), B (n = 50) and C (n = 8). In conclusion our results showed that Genotype D was the commonest genotype in Uttar Pradesh, whereas genotype A had significantly more elevated ALT/AST levels than genotype D. (P \ 0.05). Child Pugh Grade B was significantly more in patients with genotype D, whereas Child Pugh Grade C was more in genotype A.

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“…This deletion mutant has previously been reported in different clinical conditions, especially hepatocellular carcinoma (HCC) and chronic hepatitis B (CHB) infection with genotypes C and D, and fulminant hepatitis B (FHB) with genotype A in Africa. It has also been observed in isolates obtained from nonhuman primates (Table 2) [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] . Interestingly, there are no reports of this deletion mutation in genotypes B, E, F and G. In a heart transplant recipient who died from fulminate hepatitis B transmitted by the donor, the 18bp deletion was detected in the recipient, but not in the donor [20] .…”

Section: Discussionmentioning

confidence: 99%

Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

Shen¹,

Yan²,

Zou³

et al. 2008

WJG

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AIM:To determine whether HBV with the same characteristics causes dissimilar mutations in different hosts. METHODS: Full-length HBV genome was amplified and linked with pMD T18 vector. Positive clones were selected by double-restriction endonuclease digestion (Eco RⅠ and Hin dⅢ) and PCR. Twenty seven clones were randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and 6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis was performed using BioEditor, Clustal X and MEGA software. Potential immune epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method and Emini Surface Accessibility Prediction. RESULTS: All of the 27 sequences were genotype C, the divergence between the mother and son was 0%-0.8%. Compared with another 50 complete sequences of genotype C, the mother and her son each had 13 specific nucleotides that differed from the other genotype C isolates. AA 1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The 1762T/1764A double mutation existed in all clones of the mother, 3 of them were also coupled with G1896A mutation, but none were found in the son. 17 bp deletion starting at nucleotide 2330 was the major mutation (5/9) in the son, which caused seven potential HLA class Ⅰ epitopes and one B cell epitope deletion, and produced a presumptive new start codon, downstream from the original one of the P gene. CONCLUSION: The HBV strain in the son came from his mother, and discrepant mutation occurred in the mother and her son during infection.

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A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa.

Cited by 158 publications

References 23 publications

Virological outcomes in patients infected chronically with hepatitis B virus genotype A in comparison with genotypes B and C

Virological outcomes in patients infected chronically with hepatitis B virus genotype A in comparison with genotypes B and C

Distribution of Hepatitis B Virus Genotypes and Its Association with Severity of Liver Disease in Patients with Chronic Hepatitis B in Uttar Pradesh, India

Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

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