A unique role for 6-O sulfation modification in zebrafish vascular development

Chen, Eleanor; Stringer, Sally E.; Rusch, Melissa; Selleck, Scott B.; Ekker, Stephen C.

doi:10.1016/j.ydbio.2005.05.032

Cited by 78 publications

(65 citation statements)

References 41 publications

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“…6-OST-2 was expressed ubiquitously during gastrulation (Fig. 2L) and throughout somitigenesis, with strong expression in the head and presumptive brain during early somitigenesis, and in eye, hindbrain, and somitic boundaries during mid-somitigenesis, in agreement with previous reports (Bink et al, 2003;Chen et al, 2005), while expression in the tail bud was weak (Fig. 2M,N).…”

Section: -Ost-1asupporting

confidence: 91%

“…2B) and zygotic expression began during somitigenesis in polster and presumptive forebrain. During mid-somitigenesis, 6-OST-1a was expressed in eye, hindbrain, and anterior spinal cord as described previously (Chen et al, 2005) (Fig. 2C,D).…”

Section: Expression Of Zebrafish 6-ost Genessupporting

confidence: 71%

“…Zebrafish contain two genes sharing identity to human and mouse 6-OST-1. One 6-OST-1 gene has recently been reported (Chen et al, 2005). Our analysis indicates a second gene, also showing high homology to 6-OST-1 (Fig.…”

Section: Cloning Of the Zebrafish 6-osulfotransferase Familysupporting

confidence: 62%

“…1B), exists in the zebrafish genome. To avoid confusion between these two genes, we named the gene from Chen et al (2005) as 6-OST-1a and our novel 6-OST-1 gene, 6-OST-1b.…”

Section: Cloning Of the Zebrafish 6-osulfotransferase Familymentioning

confidence: 99%

“…In zebrafish, two separate phenotypes resulting from morpholino knockdown of 6-OST-2 have been reported. Bink et al reported that 6-OST (Bink et al, 2003), later renamed 6-OST-2 (Chen et al, 2005), was essential during somite specification and differentiation of muscle cells but acts independently of the somite oscillator mechanism. Chen et al reported knockdown of 6-OST-2 resulted in defects in branching morphogenesis of caudal vein (Chen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6‐O‐sulfotransferase family

Cadwallader

Yost

2006

Developmental Dynamics

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Heparan sulfate (HS) is an unbranched chain of repetitive disaccharides, which specifically binds ligands when attached to the cell surface or secreted extracellularly. HS chains contain sulfated domains termed the HS fine structure, which gives HS specific binding affinities for extracellular ligands. HS 6-Osulfotransferases (6-OST) catalyze the transfer of sulfate groups to the 6-O position of glucosamine residues of HS. We report here the characterization and developmental expression analysis of the 6-OST gene family in the zebrafish. The zebrafish 6-OST gene family consists of four conserved vertebrate orthologues, including a gene duplication specific to zebrafish. We examined the mRNA expression patterns in several tissues/organs throughout early zebrafish development, including early cleavage stages, eyes, somites, brain, internal organ primordial, and pectoral fin development. Members of the 6-OST gene family have spatially and temporally distinct restricted expression, suggesting in vivo functional differences exist between members of this family. Developmental Dynamics 235:3432-3437, 2006.

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Section: -Ost-1asupporting

confidence: 91%

Section: Expression Of Zebrafish 6-ost Genessupporting

confidence: 71%

Section: Cloning Of the Zebrafish 6-osulfotransferase Familysupporting

confidence: 62%

“…1B), exists in the zebrafish genome. To avoid confusion between these two genes, we named the gene from Chen et al (2005) as 6-OST-1a and our novel 6-OST-1 gene, 6-OST-1b.…”

Section: Cloning Of the Zebrafish 6-osulfotransferase Familymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6‐O‐sulfotransferase family

Cadwallader

Yost

2006

Developmental Dynamics

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Systemic inactivation of Hs6st1 in mice is associated with late postnatal mortality without major defects in organogenesis

Izvolsky

Lü

Martin

et al. 2008

Genesis

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Summary: Heparan sulfate (HS) proteoglycans modulate the biological activity of a number of growth factors in development, homeostasis, and cancer. Specific modifications of HS chains by HS biosynthetic enzymes have been implicated in growth factor signaling in multiple aspects of organogenesis. Although the role of HS 6-O-sulfotransferases has been described in processes such as trachea formation in Drosophila and vasculogenesis in zebrafish, little is known about how HS 6-O-sulfotransferases (Hs6st1-3 in mice) influence mouse development. To address this issue, we generated a conditionally mutant Hs6st1 mouse line and then generated mice with systemic inactivation of Hs6st1. Hs6st1-null pups were viable and grossly normal at birth. The lack of obvious abnormalities in lung, liver, and kidney, which express high levels of Hs6st1 during development, suggests that at least during embryonic life, the loss of Hs6st1 function may be compensated for by mechanisms involving other HS modifying enzymes. During early adulthood, however, Hs6st1-null mice failed to thrive and exhibited growth retardation, body weight loss, enlargement of airspaces in the lung and, in some cases, lethality. Our results suggest a potentially critical role for HS 6-O sulfation by Hs6st1 in postnatal processes. genesis 46:8-18,

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Betaglycan knock‐down causes embryonic angiogenesis defects in zebrafish

Kamaid

Molina-Villa

Mendoza

et al. 2015

Genesis

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Angiogenesis is an essential requirement for embryonic development and adult homeostasis. Its deregulation is a key feature of numerous pathologies and many studies have shown that members of the transforming growth factor beta (TGF-β) family of proteins play important roles in angiogenesis during development and disease. Betaglycan (BG), also known as TGF-β receptor type III, is a TGF-β coreceptor essential for mice embryonic development but its role in angiogenesis has not been described. We have cloned the cDNA encoding zebrafish BG, a TGF-β-binding membrane proteoglycan that showed a dynamic expression pattern in zebrafish embryos, including the notochord and cells adjacent to developing vessels. Injection of antisense morpholinos decreased BG protein levels and morphant embryos exhibited impaired angiogenesis that was rescued by coinjection with rat BG mRNA. In vivo time-lapse microscopy revealed that BG deficiency differentially affected arterial and venous angiogenesis: morphants showed impaired pathfinding of intersegmental vessels migrating from dorsal aorta, while endothelial cells originating from the caudal vein displayed sprouting and migration defects. Our results reveal a new role for BG during embryonic angiogenesis in zebrafish, which has not been described in mammals and pose interesting questions about the molecular machinery regulating angiogenesis in different vertebrates. genesis 53:583-603, 2015. © 2015 Wiley Periodicals, Inc.

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A unique role for 6-O sulfation modification in zebrafish vascular development

Cited by 78 publications

References 41 publications

Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6‐O‐sulfotransferase family

Combinatorial expression patterns of heparan sulfate sulfotransferases in zebrafish: II. The 6‐O‐sulfotransferase family

Systemic inactivation of Hs6st1 in mice is associated with late postnatal mortality without major defects in organogenesis

Betaglycan knock‐down causes embryonic angiogenesis defects in zebrafish

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