2013
DOI: 10.1038/ncb2680
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A unique Oct4 interface is crucial for reprogramming to pluripotency

Abstract: Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker bet… Show more

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Cited by 144 publications
(225 citation statements)
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“…This result contrasts former observations reporting no iPSC colonies for Oct4 LinkO6 constructs 43, 44. This discrepancy can be explained by a structural alignment of the linker sequences, in which the entire RK motifs of Oct4 and Oct6 are aligned with a central gap causing an Arg residue to be part of the swapped linker in one but not the other construct (Appendix Fig S1A).…”
Section: Discussioncontrasting
confidence: 90%
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“…This result contrasts former observations reporting no iPSC colonies for Oct4 LinkO6 constructs 43, 44. This discrepancy can be explained by a structural alignment of the linker sequences, in which the entire RK motifs of Oct4 and Oct6 are aligned with a central gap causing an Arg residue to be part of the swapped linker in one but not the other construct (Appendix Fig S1A).…”
Section: Discussioncontrasting
confidence: 90%
“…POU S , the linker region, and POU HD , as well as individual helixes, are labeled. Notably, this structure‐based alignment differs slightly from the one used previously 44, which is explained by a low conservation of residues in the N‐terminal part of the POU linker. The alignment was prepared in T‐Coffee software and colors distinguish conservation and amino acid residue types.…”
Section: Resultsmentioning
confidence: 69%
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