A unique fluorescent probe for visualization of cell death via its subcellular immigration from lysosomes to nucleus

Ge, Enxiang; Tian, Minggang; Lin, Weiying

doi:10.1016/j.snb.2021.130656

Cited by 9 publications

(6 citation statements)

References 31 publications

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“…Further, the process of drug-induced cell death was assessed by PNE-Lyso . Rotenone, as a toxin, was used to stimulate cell death . In the cells incubated with rotenone, the fluorescence of PNE-Lyso experienced a ratiometric change between red and yellow channels, and the red fluorescence of PNE-Lyso completely disappeared when the cells were incubated with rotenone for 12 h (Figure S21), indicating that rotenone induced cell death.…”

Section: Resultsmentioning

confidence: 99%

“…Rotenone, as a toxin, was used to stimulate cell death. 44 In the cells incubated with rotenone, the fluorescence of PNE-Lyso experienced a ratiometric change between red and yellow channels, and the red fluorescence of PNE-Lyso completely disappeared when the cells were incubated with rotenone for 12 h (Figure S21), indicating that rotenone induced cell death. Continuing to extend the incubation time, the number of granular lysosomes gradually decreased and the morphology became blurred, resulting in that the yellow fluorescence of PNE-Lyso was distributed in the cytoplasm (Figure 7g).…”

Section: Design Strategy and Study Of Spectra Of Pohmentioning

confidence: 99%

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Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology

et al. 2022

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Cell death plays a vital role in body development, maintenance of tissue function, and homeostasis. Accurate evaluation of cell death types is of great importance for pharmacological and pathological research. However, there is a lack of efficient fluorescent probes to discriminate various cell states. Here, we design and synthesize a novel activatable fluorescent probe PNE-Lyso to detect intracellular pH and hexosaminidases with two kinds of fluorescence signals. PNE-Lyso could distinguish dead cells from healthy cells based on a dual-color mode by targeting the lysosome and evaluating lysosomal hexosaminidase activity. Significantly, PNE-Lyso could also discriminate apoptotic and necrotic cells through visualizing lysosome morphology that is adjusted by the integrity of the lysosome membrane. Moreover, probe PNE-Lyso was successfully applied to investigate the drug-induced cell death process. To the best of our knowledge, this work is the first time cell death types have been distinguished based on a single fluorescent probe.

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Section: Resultsmentioning

confidence: 99%

Section: Design Strategy and Study Of Spectra Of Pohmentioning

confidence: 99%

Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology

et al. 2022

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“…Benefitting from the overexpressed esterase in cancer cells, TPE-QC could be efficiently activated in cancer cells rather than in normal cells, resulting in enhanced anticancer potency. Lin’s group synthesized a fluorescent probe, LN-2 ( 64 , Scheme 7 ), to visualize cell death based on its subcellular immigration from lysosomes towards the nucleus by regulating the binding affinity of the probe to lysosomes and DNA [ 179 ]. LN-2 was successfully used to display cell death induced by hydrogen peroxide and apoptosis induced by rotenone.…”

Section: Design Of Molecular Probes For Organelle-targeted Cell Imagingmentioning

confidence: 99%

Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging

Dai

Cui

et al. 2023

Biosensors

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Fluorescent molecular probes are very powerful tools that have been generally applied in cell imaging in the research fields of biology, pathology, pharmacology, biochemistry, and medical science. In the last couple of decades, numerous molecular probes endowed with high specificity to particular organelles have been designed to illustrate intracellular images in more detail at the subcellular level. Nowadays, the development of cell biology has enabled the investigation process to go deeply into cells, even at the molecular level. Therefore, probes that can sketch a particular organelle’s location while responding to certain parameters to evaluate intracellular bioprocesses are under urgent demand. It is significant to understand the basic ideas of organelle properties, as well as the vital substances related to each unique organelle, for the design of probes with high specificity and efficiency. In this review, we summarize representative multifunctional fluorescent molecular probes developed in the last decade. We focus on probes that can specially target nuclei, mitochondria, endoplasmic reticulums, and lysosomes. In each section, we first briefly introduce the significance and properties of different organelles. We then discuss how probes are designed to make them highly organelle-specific. Finally, we also consider how probes are constructed to endow them with additional functions to recognize particular physical/chemical signals of targeted organelles. Moreover, a perspective on the challenges in future applications of highly specific molecular probes in cell imaging is also proposed. We hope that this review can provide researchers with additional conceptual information about developing probes for cell imaging, assisting scientists interested in molecular biology, cell biology, and biochemistry to accelerate their scientific studies.

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“…It is well known that autophagy and apoptosis are lysosome-dependent pathophysiological processes, which would in turn affect lysosomal properties. Lysosomal pH is significantly decreased during autophagy but evidently increased during apoptosis. − By these facts, in this work, a versatile single fluorescent probe for the ratiometric and discriminative visualization of autophagy and apoptosis based on the aggregation/monomer principle has been developed. As illustrated in Scheme a, the probe could simultaneously and separately target lysosomes and RNA with different fluorescent colors.…”

Section: Introductionmentioning

confidence: 99%

Ratiometric and Discriminative Visualization of Autophagy and Apoptosis with a Single Fluorescent Probe Based on the Aggregation/Monomer Principle

et al. 2022

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Autophagy and apoptosis play a central role in maintaining homeostasis in mammals. Therefore, discriminative visualization of the two cellular processes is an important and challenging task. However, fluorescent probes enabling ratiometric visualization of both autophagy and apoptosis with different sets of fluorescence signals have not been developed yet. In this work, we constructed a versatile single fluorescent probe (NKLR) based on the aggregation/monomer principle for the ratiometric and discriminative visualization of autophagy and apoptosis. NKLR can simultaneously perform two-color imaging of RNA (deep red channel) and lysosomes (yellow channel) in aggregation and monomer states, respectively. During autophagy, NKLR migrated from cytoplasmic RNA and nuclear RNA to lysosomes, showing enhanced yellow emission and sharply decreased deep red fluorescence. Moreover, this migration process was reversible upon the recovery of autophagy. Comparatively, during apoptosis, NKLR immigrated from lysosomes to RNA, and the yellow emission decreased and even disappeared, while the fluorescence of the deep red channel slightly increased. Overall, autophagy and apoptosis could be discriminatively visualized via the fluorescence intensity ratios of the two channels. Meanwhile, the cells in three different states (healthy, autophagic, apoptotic) could be distinguished by three point-to-point fluorescence images via the localization and emission color of NKLR. Therefore, the probe NKLR can serve as a desirable molecular tool to reveal the in-depth relation between autophagy and apoptosis and facilitate the study on the two cellular processes.

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A unique fluorescent probe for visualization of cell death via its subcellular immigration from lysosomes to nucleus

Cited by 9 publications

References 31 publications

Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology

Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology

Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging

Ratiometric and Discriminative Visualization of Autophagy and Apoptosis with a Single Fluorescent Probe Based on the Aggregation/Monomer Principle

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