A unigene catalogue of 5700 expressed genes in cassava

López, Camilo; Jorge, Véronique; Piégu, Benoît; Chickelu,; Cortes, Diego Fernando Marmolejo; Restrepo, Silvia; Soto, Mauricio; Laudié, Michèle; Berger, Christel; Cooke, Richard; Delseny, Michel; Tohmé, Joe; Verdier, Valérie

doi:10.1007/s11103-004-0123-4

Cited by 56 publications

(43 citation statements)

References 41 publications

(46 reference statements)

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“…Very little information is available about the protein coded by EG5651 in cassava or in any other plant species. This is not unexpected as Lopez et al (2004) were only able to assign likely biochemical function to 37% of unigenes in their study of 5,800 cassava unigenes. Furthermore, some 16% of their sequences showed no homology to known sequences and were thus likely to be cassavaspecific.…”

Section: Discussionmentioning

confidence: 92%

Identification of genes that have undergone adaptive evolution in cassava (Manihot esculenta) and that may confer resistance to cassava brown streak disease

Ferguson¹,

Koga²,

Jiang³

et al. 2015

Afr. J. Biotechnol.

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Section: Discussionmentioning

confidence: 92%

Identification of genes that have undergone adaptive evolution in cassava (Manihot esculenta) and that may confer resistance to cassava brown streak disease

Ferguson¹,

Koga²,

Jiang³

et al. 2015

Afr. J. Biotechnol.

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“…Two main approaches have been used: based on hybridization and sequencing. Cassava is one of the very few African staple food crop to which microarrays have been applied [31][32][33][34][35][36].…”

Section: Ngs-based Gene Expression Analysismentioning

confidence: 99%

Perspectives on the Application of Next-generation Sequencing to the Improvement of Africa’s Staple Food Crops

Gedil¹,

Ferguson²,

Girma³

et al. 2016

Next Generation Sequencing - Advances, Applications and Challenges

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The persistent challenge of insufficient food, unbalanced nutrition, and deteriorating natural resources in the most vulnerable nations, characterized by fast population growth, calls for utilization of innovative technologies to curb constraints of crop production. Enhancing genetic gain by using a multipronged approach that combines conventional and genomic technologies for the development of stress-tolerant varieties with high yield and nutritional quality is necessary. The advent of nextgeneration sequencing (NGS) technologies holds the potential to dramatically impact the crop improvement process. NGS enables whole-genome sequencing (WGS) and re-sequencing, transcriptome sequencing, metagenomics, as well as high-throughput genotyping, which can be applied for genome selection (GS). It can also be applied to diversity analysis, genetic and epigenetic characterization of germplasm and pathogen detection, identification, and elimination. High-throughput phenotyping, integrated data management, and decision support tools form the necessary supporting environment for effective utilization of genome sequence information. It is important that these opportunities for mainstreaming innovative breeding strategies, enabled by cutting-edge "Omics" technologies, are seized in Africa; however, several constraints must be addressed before the benefit of NGS can be fully realized. African breeding programs must have access to high-throughput genotyping facilities, capacity in the application of genome selection and marker-assisted breeding must be built and supported by capacity in genomic analysis and bioinformatics. This chapter demonstrates how interventions with NGS-enabled innovative strategies can be applied to increase genetic gain with insights from the Consortium of International

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“…An initiative towards completing the saturation of the cassava genetic map has also resulted in the generation of expressed sequence tags (ESTs) and SNPs. Several ESTs have been developed for cassava (Lopez et al, 2004;Lokko et al, 2007) with over 80,000 ESTs for cassava available in the Genbank. Over 1700 cassava SNPs are now available for molecular studies (Pablo et al, unpublished; Morag et al, unpublished).…”

Section: Genome Mapping and Gene Discoverymentioning

confidence: 99%

Cassava Genetic Improvement

Ceballos¹,

Fregene²,

Carlos³

et al. 2007

Breeding Major Food Staples

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PREFACEThis Handbook is the result of a combined effort by several current and previous cassava researchers at CIAT to review and summarize the most important results of cassava research during the past 40 years. Most of the chapters are based on the various presentations during the Regional Cassava Training Course, held in Thailand from October 6 to 17, 2008. This course was organized upon the realization that many people that were actively involved with cassava research in the 1970s and 80s, both at CIAT and in national programs, have now retired or will soon do so, and that a whole new generation of cassava researchers are currently being hired to take their place.As cassava is now becoming a very important, and mostly industrial, crop in Asia, there are many new opportunities, but also a host of new problems and challenges. These include the appearance in Asia of new cassava diseases and pests; the decreasing availability and increasing cost of rural labor, resulting in the need for partial or complete mechanization of cassava production; the rapidly increasing demand for cassava roots for production of food, feed and fuel, and the unavailability in many countries of new land for any expansion of cassava area, thus requiring a rapid increase in cassava yields to increase supplies. This requires a renewed focus on cassava research for the development of new higher-yielding varieties and more sustainable production practices.While many cassava researchers in national programs in Asia received individual or group training at CIAT-Colombia during the 1970s and 80s, this training was greatly reduced during the following two decades due to funding limitations. Thus, the objective of the Regional Cassava Training Course in 2008 was to provide a new training opportunity for young scientists in Asian countries, and to hand over the knowledge and experience of the older cassava researchers, mostly from CIAT, to a new generation that will have to face the new challenges. Thus, the course was taught mostly by current or already retired CIAT cassava researchers, while the 60-plus participants of the course included cassava researchers from Cambodia, China, East Timor, India, Indonesia, Lao PDR, Malaysia, Myanmar, the Philippines, Thailand and Vietnam. The training course not only provided knowledge -from physiology and biotechnology to animal feeding and production of fuelethanol -but also an opportunity for people from different institutions and countries to get to know each other, which will greatly facilitate future collaboration.Course participants returned home with a CD containing the PowerPoint presentations of the course. However, it was felt that a more comprehensive review of all the topics covered was warranted, as this would provide more in-depth knowledge for those working in the various specialized fields. Most of this information is available in many refereed journals, in workshop proceedings and old CIAT annual reports, but many of these are now out of print or otherwise difficult to obtain. Thus, the Cas...

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A unigene catalogue of 5700 expressed genes in cassava

Cited by 56 publications

References 41 publications

Identification of genes that have undergone adaptive evolution in cassava (Manihot esculenta) and that may confer resistance to cassava brown streak disease

Identification of genes that have undergone adaptive evolution in cassava (Manihot esculenta) and that may confer resistance to cassava brown streak disease

Perspectives on the Application of Next-generation Sequencing to the Improvement of Africa’s Staple Food Crops

Cassava Genetic Improvement

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