A unifying hypothesis on the central role of reactive oxygen species in bacterial pathogenesis and host defense in C. elegans

Goswamy, Debanjan; Irazoqui, Javier E.

doi:10.1016/j.coi.2020.08.002

Cited by 17 publications

(9 citation statements)

References 90 publications

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“…Excessive levels of ROS can cause oxidative stress and cellular damage in organisms . Compound A7 at 2, 5, and 10 mg/L caused a significant increase in the ROS levels in B.…”

Section: Resultsmentioning

confidence: 99%

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Design, Synthesis, Nematicidal Activity, and Mechanism of Novel Amide Derivatives Containing an 1,2,4-Oxadiazole Moiety

Wang,

Song,

Wang

et al. 2023

J. Agric. Food Chem.

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To discover new nematicides, a series of novel amide derivatives containing 1,2,4-oxadiazole were designed and synthesized. Several compounds showed excellent nematicidal activity. The LC 50 values of compounds A7, A18, and A20−A22 against pine wood nematode (Bursaphelenchus xylophilus), rice stem nematode (Aphelenchoides besseyi), and sweet potato stem nematode (Ditylenchus destructor) were 1.39−3.09 mg/L, which were significantly better than the control nematicide tioxazafen (106, 49.0, and 75.0 mg/L, respectively). Compound A7 had an outstanding inhibitory effect on nematode feeding, reproductive ability, and egg hatching. Compound A7 effectively promoted the oxidative stress of nematodes and caused intestinal damage to nematodes. Compound A7 significantly inhibited the activity of succinate dehydrogenase (SDH) in nematodes, leading to blockage of electron transfer in the respiratory chain and thereby hindering the synthesis of adenosine triphosphate (ATP), which consequently affects the entire oxidative phosphorylation process to finally cause nematode death. Therefore, compound A7 can be used as a potential SDH inhibitor in nematicide applications.

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“…Excessive levels of ROS can cause oxidative stress and cellular damage in organisms . Compound A7 at 2, 5, and 10 mg/L caused a significant increase in the ROS levels in B.…”

Section: Resultsmentioning

confidence: 99%

“…28 oxidative stress and cellular damage in organisms. 41 Compound A7 at 2, 5, and 10 mg/L caused a significant increase in the ROS levels in B. xylophilus (Figure 5A,B). As the concentration of compound A7 gradually increased, the level of ROS in the nematodes also increased.…”

Section: ■ Materials and Methodsmentioning

confidence: 97%

Design, Synthesis, Nematicidal Activity, and Mechanism of Novel Amide Derivatives Containing an 1,2,4-Oxadiazole Moiety

Wang,

Song,

Wang

et al. 2023

J. Agric. Food Chem.

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“…ROS, which include hydroxyl radicals, superoxide anions, and hydrogen peroxide, are generated from oxygen reduction. While excessive ROS can be toxic to cells ( 74 – 77 ), they also serve a protective function by killing invading bacteria and fungi ( 72 , 78 ), suggesting dual roles in innate immunity. Recent studies demonstrated that ROS also affect pathogen avoidance ( Figure 1 ).…”

Section: Signalling Crosstalk Between Pathogen Avoidance and Innate I...mentioning

confidence: 99%

Neuronal basis and diverse mechanisms of pathogen avoidance in Caenorhabditis elegans

Lei,

Tan,

et al. 2024

Front. Immunol.

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Pathogen avoidance behaviour has been observed across animal taxa as a vital host-microbe interaction mechanism. The nematode Caenorhabditis elegans has evolved multiple diverse mechanisms for pathogen avoidance under natural selection pressure. We summarise the current knowledge of the stimuli that trigger pathogen avoidance, including alterations in aerotaxis, intestinal bloating, and metabolites. We then survey the neural circuits involved in pathogen avoidance, transgenerational epigenetic inheritance of pathogen avoidance, signalling crosstalk between pathogen avoidance and innate immunity, and C. elegans avoidance of non-Pseudomonas bacteria. In this review, we highlight the latest advances in understanding host-microbe interactions and the gut-brain axis.

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“…All organisms have developed mechanisms to cope with stress, and failure to maintain appropriate levels of ROS usually causes oxidative stress and cellular damage. 46,74,75 According to a recent publication, C. elegans' growth, locomotion behavior, feeding, lifespan, and reproduction can be harmed by sublethal exposure to flu, which also induces oxidative stress, as indicated by an increase in ROS production. 30 To explore the mechanism of SPc-based bioactivity enhancement, oxidative stress was also investigated by measuring ROS production.…”

Section: Amplified Adverse Impacts Of Flu Nanoagents On Oxidative Str...mentioning

confidence: 99%

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation

Peng

Jian

Long

et al. 2023

ACS Appl. Mater. Interfaces

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Long-term overuse of chemical nematicides has resulted in low control efficacy toward destructive root-knot nematodes, and continuous development in nanotechnology is supposed to enhance the utilization efficiency of nematicides to meet practical needs. Herein, a cationic star polymer (SPc) was constructed to load fluopyram (flu) and prepare a flu nanoagent. Hydrogen bonding and van der Waals forces facilitated the self-assembly of the flu nanoagent, leading to the breakdown of self-aggregated flu and reducing its particle size to 60 nm. The bioactivity of flu was remarkably improved, with the half lethal concentration 50 from 8.63 to 5.70 mg/L due to the help of SPc. Transcriptome analysis found that a large number of transport-related genes were upregulated in flu nanoagent-exposed nematodes, while the expression of many energy-related genes was disturbed, suggesting that the enhanced uptake of flu nanoagents by nematodes might lead to the disturbance of energy synthesis and metabolism. Subsequent experiments confirmed that exposure to flu nanoagents markedly increased the reactive oxygen species (ROS) level of nematodes. Compared to flu treatment alone, succinate dehydrogenase (SDH) activity was inhibited in flu nanoagent-exposed nematodes with an increase in the pIC50 from 8.81 to 11.04, which further interfered with adenosine triphosphate (ATP) biosynthesis. Furthermore, the persistence of SPc-loaded flu in soil was prolonged by 2.33 times at 50 days after application. The protective effects of flu nanoagents on eggplant seedlings were significantly improved in both greenhouse and field trials, and the root-knot number was consistently smaller in roots treated with flu nanoagents than in those treated with flu alone. Overall, this study successfully constructed a self-assembled flu nanoagent with amplified effects on oxidative stress, SDH activity, and ATP generation, leading to highly effective control of root-knot nematodes in the field.

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A unifying hypothesis on the central role of reactive oxygen species in bacterial pathogenesis and host defense in C. elegans

Cited by 17 publications

References 90 publications

Design, Synthesis, Nematicidal Activity, and Mechanism of Novel Amide Derivatives Containing an 1,2,4-Oxadiazole Moiety

Design, Synthesis, Nematicidal Activity, and Mechanism of Novel Amide Derivatives Containing an 1,2,4-Oxadiazole Moiety

Neuronal basis and diverse mechanisms of pathogen avoidance in Caenorhabditis elegans

Self-Assembled Nanonematicide Induces Adverse Effects on Oxidative Stress, Succinate Dehydrogenase Activity, and ATP Generation

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