A unified method for the determination of gold in biological fluids by flameless atomic absorption spectroscopy

Pedersen, Susanne Møller; Graabæk, Pernille Møller

doi:10.3109/00365517709108810

Cited by 14 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytoplasmic accumulation of GST was expressed as the GST concentration measured by ftameless atomic absorption spectroscopy in the supernatant of the cell 6 lysate [13]. Both 2 x 10 THP-1/GST and THP-1 were incubated in 10 % FCS and RPMI 1640 for 48 h in the absence of GST.…”

Section: Cell Accumulation Of Gstmentioning

confidence: 99%

Induction of drug resistance to gold sodium thiomalate in a monocyte cell line, THP-1

et al. 1998

View full text Add to dashboard Cite

The expression of metallothionein, an intracellular heavy-metal-binding protein, and p-glycoprotein, an energy-dependent drug efflux pump, was examined to study the mechanism of cell resistance to gold sodium thiomalate (GST). THP-1, one of the monocyte-derived cell lines, was cultured for 6 months and resistance to 25 microg/ml of GST (GST-resistant cells) was thus induced. The GST-resistant cells were then cultured with bucillamine to examine the presence of cross-resistance. The intracellular GST concentration was examined by flameless atomic absorption spectroscopy. The cell viability was determined by the uptake of 3-4,5 dimethylthiazole-2,5 diphenyl tetrazolium bromide (MTT). The expression of p-glycoprotein was detected by Western blotting using monoclonal anti-p-glycoprotein antibody. The expression of metallothionein was detected using the indirect immunofluorescence technique. GST-resistant cells did not show any cross-resistance to bucillamine. The rate of cytoplasmic GST accumulation decreased in the GST-resistant cells, while the rate of GST efflux also decreased. The expression of p-glycoprotein in the GST-resistant cells was not significantly different from that in the cells not treated with GST. On the other hand, the GST-resistant cells showed a higher expression of metallothionein than cells not treated with GST. These findings suggest that the induced resistance to GST might partly be due to an induction of metallothionein.

show abstract

Section: Cell Accumulation Of Gstmentioning

confidence: 99%

Induction of drug resistance to gold sodium thiomalate in a monocyte cell line, THP-1

et al. 1998

View full text Add to dashboard Cite

show abstract

“…At day 9, the cells were incubated in AuNP and ethanol free culture media for another 16 days. At predetermined times (day 9 to day 25 (i.e., day 0 days post ethanol), cells were harvested and processed for the following: Determination of internalized AuNPs by measuring intracellular Au concentrations (iAuCs): the atomic absorption spectrometric method described by Pedersen and Graabaek [ 36 ] and Benlhabib, et al [ 13 , 14 ] was used for analysis of Au in tissue samples. In brief, the cells were harvested, treated with trypsin, etched with potassium iodide (KI) and iodine (I 2 ) at 1:6 ratio (0.34 mM I 2 ) to remove the surface AuNPs [ 37 ], washed with plasma buffered saline, and mixed with 5 mL of aqua regia, and digested for 24 h. The digest was diluted 1:1 with a solution of 2 ppm yttrium in dilute nitric acid, serving as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

Comparative Therapeutic Effects of Plant-Extract Synthesized and Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory Activity in SH-SY5Y Cells In Vitro

Singh

2017

Biomedicines

View full text Add to dashboard Cite

The present study describes potential beneficial and adverse effects of plant-extract synthesized gold nanoparticles (AuNPs) on ethanol toxicity in SH-SY5Y cells. Although kudzu root extract (K), edible-gum extract (G), alone or in combination (KG), reduced Au3+ into AuNPs, the extract’s composition and the reaction temperature determined their size (AuNPKG(90<50<37) << AuNPK (90,50<37) < AuNPG (90<50); the subscript KG, K, or G is extract identification and numerical vales are reaction temperature in Celsius) and biological properties (AuNPKG (90,50>37) << AuNPK (90,50>37) < AuNPG (90,50)). The surface of each AuNP contained the extract’s active ingredients, that were analyzed and confirmed using laser desorption ionization (LDI)) and low-matrix laser desorption-ionization (LMALDI). AuNPKG-50 was (i) least toxic to SH-SY5Y cells, but most effective in suppressing the adverse effects of ethanol on SH-SY5Y cells, and (ii) more effective than a combination of free kudzu and gum extracts. The beneficial and adverse effects of AuNPs may have been modified by the formation of proteins corona. This study provides a proof of concept for possible application of plant-extract synthesized AuNPs in mitigating ethanol toxicity.

show abstract

The binding of gold to human albumin in vitro. Intrinsic association constants at physiological conditions

Pedersen

1981

Biochemical Pharmacology

View full text Add to dashboard Cite

A unified method for the determination of gold in biological fluids by flameless atomic absorption spectroscopy

Cited by 14 publications

References 0 publications

Induction of drug resistance to gold sodium thiomalate in a monocyte cell line, THP-1

Induction of drug resistance to gold sodium thiomalate in a monocyte cell line, THP-1

Comparative Therapeutic Effects of Plant-Extract Synthesized and Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory Activity in SH-SY5Y Cells In Vitro

The binding of gold to human albumin in vitro. Intrinsic association constants at physiological conditions

Contact Info

Product

Resources

About