A type 2 metallothionein (SbMT-2) gene cloned from Salicornia brachiata confers enhanced Zn stress-tolerance in transgenic tobacco by transporting Zn2+ and maintaining photosynthesis efficacy

Patel, Manish Kumar; Pandey, Sonika; Patel, Jaykumar; Mishra, Avinash

doi:10.1016/j.envexpbot.2021.104626

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…For the Na + and K + contents, the dry leaf samples (100 mg) were digested in perchloric acid and nitric acid (9:4 v / v ratio), evaporated to dryness on a hotplate, and the residue was dissolved in 50 mL deionized water. The solution was filtered through 0.22 µm syringe filter before measuring the Na + and K + contents with an inductively coupled plasma atomic absorption spectrometer (Optima 2000DV, Perkin Elmer, Waltham, MA, USA) [ 69 ].…”

Section: Methodsmentioning

confidence: 99%

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Patel

Khandwal

Choudhary

et al. 2022

IJMS

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The frequency and severity of extreme climatic conditions such as drought, salinity, cold, and heat are increasing due to climate change. Moreover, in the field, plants are affected by multiple abiotic stresses simultaneously or sequentially. Thus, it is imperative to compare the effects of stress combinations on crop plants relative to individual stresses. This study investigated the differential regulation of physio-biochemical and metabolomics parameters in peanut (Arachis hypogaea L.) under individual (salt, drought, cold, and heat) and combined stress treatments using multivariate correlation analysis. The results showed that combined heat, salt, and drought stress compounds the stress effect of individual stresses. Combined stresses that included heat had the highest electrolyte leakage and lowest relative water content. Lipid peroxidation and chlorophyll contents did not significantly change under combined stresses. Biochemical parameters, such as free amino acids, polyphenol, starch, and sugars, significantly changed under combined stresses compared to individual stresses. Free amino acids increased under combined stresses that included heat; starch, sugars, and polyphenols increased under combined stresses that included drought; proline concentration increased under combined stresses that included salt. Metabolomics data that were obtained under different individual and combined stresses can be used to identify molecular phenotypes that are involved in the acclimation response of plants under changing abiotic stress conditions. Peanut metabolomics identified 160 metabolites, including amino acids, sugars, sugar alcohols, organic acids, fatty acids, sugar acids, and other organic compounds. Pathway enrichment analysis revealed that abiotic stresses significantly affected amino acid, amino sugar, and sugar metabolism. The stress treatments affected the metabolites that were associated with the tricarboxylic acid (TCA) and urea cycles and associated amino acid biosynthesis pathway intermediates. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and heatmap analysis identified potential marker metabolites (pinitol, malic acid, and xylopyranose) that were associated with abiotic stress combinations, which could be used in breeding efforts to develop peanut cultivars that are resilient to climate change. The study will also facilitate researchers to explore different stress indicators to identify resistant cultivars for future crop improvement programs.

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Section: Methodsmentioning

confidence: 99%

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Patel

Khandwal

Choudhary

et al. 2022

IJMS

Self Cite

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“…The prepared cartridges were scanned using an Affymetrix chip scanner and the GeneChip command console program. Data were exported in CEL and Excel file formats for subsequent gene expression analysis following Patel et al, 2021.…”

Section: Methodsmentioning

confidence: 99%

SbPIP2Mediated Improvements in Plant Resilience: Physiological and Molecular Insights into Abiotic Stress Response

Patel,

Khatri,

Gupta

et al. 2024

Preprint

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Understanding the mechanisms behind plant resilience to abiotic stresses is essential for enhancing crop yield and sustainability. This study integrates findings from a comprehensive investigation into the function of the SbPIP2 gene, which encodes an aquaporin protein, in improving the abiotic stress tolerance of transgenic plants. Our integrated approach revealed that transgenic plants overexpressing SbPIP2 significantly reduce reactive oxygen species (ROS) accumulation and exhibit enhanced physiological attributes, including higher seed germination rates, improved growth, early flowering, and better seed setting under stress conditions. Notably, these plants also showed a quicker recovery and completion of their lifecycle post-stress treatment. The transcriptomic analysis provided a deeper understanding of the genetic modifications contributing to stress resilience, highlighting the involvement of genes associated with oxidative stress response, calcium and sugar signaling pathways, stomatal regulation, phytohormone biosynthesis, and flower development. Additionally, the study underscores the central role of abscisic acid (ABA) in mediating stress responses through hormonal regulation, with transgenic plants displaying increased ABA levels due to the upregulation of biosynthesis genes and downregulation of catabolism genes. This hormonal adjustment is critical for stomatal closure, reducing water loss, and enhancing tolerance to abiotic stresses. Our findings elucidate the complex genetic and molecular pathways that underpin abiotic stress tolerance in plants, offering valuable insights for future research aimed at improving crop resilience through genetic engineering, thereby addressing the challenges of climate change and environmental stressors.

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“…Nuclearly have been expressed genes to promote the tolerance to different abiotic stresses such as cold stress (CsWRKY46 gene) [154], oxidative stress (ScVTC2 gene) [155], drought and salt stress tolerance (ZmSNAC13 and ZmWRKY86 gene, respectively) [156,157], metal tolerance (OsMYB-R1 and SbMT-2 gene) [158][159][160], ROS decrease (CfAPX gene) [160] and tolerance to waterlogging (HaOXR2 gene) [161] and have been obtained satisfactory results; nevertheless, trying to improve the results obtained by nuclear expression, genes have been expressed in chloroplasts aimed at increasing the antioxidant pathway, intervening in the glycine betaine (GB) pathway such as codA gene from Arthrobacter globiformis [98, 162,163].…”

Section: Chloroplast Transformation To Confer Abiotic Stress Tolerancementioning

confidence: 99%

Chloroplasts: The Future of Large-Scale Protein Production

Chávez¹,

Ornelas²,

Cruz³

et al. 2024

Physiology

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Chloroplast engineering has matured considerably in recent years. It is emerging as a promising tool to address the challenges related to food security, drug production, and sustainable energy posed by an ever-growing world population. Chloroplasts have proven their potential by efficiently expressing transgenes, encapsulating recombinant proteins, and protecting them from cellular machinery, making it possible to obtain highly functional proteins. This quality has also been exploited by interfering RNA technology. In addition to the practical attributes offered by chloroplast transformation, such as the elimination of position effects, polycistronic expression, and massive protein production, the technique represents an advance in biosafety terms; however, even if its great biotechnological potential, crops that have efficiently transformed are still a proof of concept. Despite efforts, other essential crops have remained recalcitrant to chloroplast transformation, which has limited their expansion. In this chapter, we address the most recent advances in this area and the challenges that must be solved to extend the transformation to other crops and become the de facto tool in plant biotechnology.

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A type 2 metallothionein (SbMT-2) gene cloned from Salicornia brachiata confers enhanced Zn stress-tolerance in transgenic tobacco by transporting Zn2+ and maintaining photosynthesis efficacy

Cited by 11 publications

References 45 publications

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

SbPIP2Mediated Improvements in Plant Resilience: Physiological and Molecular Insights into Abiotic Stress Response

Chloroplasts: The Future of Large-Scale Protein Production

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