A two‐step model of T cell subset commitment: antigen‐independent commitment of T cells before encountering nominal antigen during pathogenic infections

Kanoh, Makoto; Uetani, Teruyoshi; Sakan, Hirokazu; Maruyama, Saho; Liu, Fengzhi; Sumita, Kohsuke; Asano, Yoshihiro

doi:10.1093/intimm/dxf024

Cited by 8 publications

(34 citation statements)

References 38 publications

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“…3c). This is consistent with our previous observation that the Lm-infection increased the precursor frequency of type 1 T cells (14). Since APC used in the present experiment were of uninfected mice origin and predominantly induced the type 2 T cell subset under experimental conditions, the observed effect of infection found in TCR-Tg T cells was thought to be created before exposure to nominal antigen.…”

Section: A Shift In Cd4 T Cell Subsets Is Induced Before Exposure To supporting

confidence: 93%

“…In addition, this CD4 T cell activation event does not require the additional bystander T cells or B cells. As shown in our previous report (14), Lminfection induces a shift in precursor frequency of CD4 T cell subsets in an antigen-independent manner and resulted in a predominant shift to type 1 CD4 T cells in RAG-1 Ϫ/Ϫ OVA-TCR-Tg mice during Lm-infection (Fig. 3c).…”

Section: Discussionsupporting

confidence: 81%

“…The shift has been thought to be antigen-dependent and therefore TCR mediated (1,8,10,12,13,15,18,20,22,23,25,31,37,38). In a previous study, however, we demonstrated that the shift occurs before T cells encounter nominal antigen during bacterial infection, i.e., we showed one of the initial induction step for CD4 T cell subsets during Listeria monocytogenes (Lm) infection is induced by the basal function of macrophage/dendritic cells and is antigen-independent and antigen-nonspecific (14). This initial induction step affects the precursor frequency of type 1 and type 2 CD4 T cell subsets.…”

mentioning

confidence: 78%

“…L. monocytogenes (EGD strain, Lm) was provided by Dr. P. Cossart, Pasteur Institute, Paris, France. For in vivo infection experiments otherwise stated in the text, 3 ϫ 10 3 of bacteria was inoculated intraperitoneally (14).…”

Section: Methodsmentioning

confidence: 99%

“…After 5 days of cultivation at 37 C in a 5% CO 2 humidified air atmosphere, cells were collected and washed, and re-stimulated with APCs and homologous antigen for 2 days. The amounts of IL-4 and IFN-γ in the culture supernatant were determined by ELISA assay (8,14,37).…”

Section: Methodsmentioning

confidence: 99%

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Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells

Maruyama

Shen

Kanoh

et al. 2007

Microbiology and Immunology

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Bacterial infection induces a shift to type 1 CD4 T cell subset in an infected host and this shift is important for protection of the host from disease development. Many researchers think that the shift is antigen-dependent, but we previously demonstrated an initial induction step for CD4 T cell subsets during Listeria monocytogenes (Lm) infection is antigen-independent. Although Listeria is a TLR2 ligand, the immune system of the Lm-infected host responded to the pathogen to induce expression of CD69 but not CD25 on CD4 T cells, CD8 T cells and B cells even in the absence of TLR2 or MyD88. The antigen-independent activation of type 1 CD4 T cells accelerate the clearance of pathogens by activating innate immune cells with type 1 cytokines. Type 1 CD4 T cells and CD8 T cells also collaborate to protect the host from intracellular Lm infection. Since CD8 T cells function mainly as cytotoxic T cells and CD69-positive CD8 T cells increase during Lm-infection, cytotoxic activity of CD8 T cells was evaluated during Lm-infection. Although CD8 T cells were activated to produce IFN-γ γ, the cytotoxic function of CD8 T cells in Lymphocytic choriomeningitis virus (LCMV) p14 TCR-transgenic mouse was not augmented by Lm-infection.Therefore, Lm-infection differentially influences on cytokine production and cytotoxicity of CD8 T cells.

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Section: A Shift In Cd4 T Cell Subsets Is Induced Before Exposure To supporting

confidence: 93%

Section: Discussionsupporting

confidence: 81%

mentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells

Maruyama

Shen

Kanoh

et al. 2007

Microbiology and Immunology

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Roles for CD40, B7 and major histocompatibility complex in induction of enhanced immunity by cryptococcal polysaccharide‐pulsed antigen‐presenting cells

Blackstock

2003

Immunology

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SUMMARYImmunization of mice with activated antigen-presenting cells (APC) pulsed ex vivo with cryptococcal capsular polysaccharide, a glucuronoxylomannan (GXM-APC) results in prolongation of survival and delayed-type hypersensitivity (DTH) responsiveness following infection with Cryptococcus neoformans (NU-2). GXM-APC has both non-specific and GXM-specific effects that influence the immune responses that develop in mice after infection with NU-2. Type 1 cytokine responses are augmented after immunization with APC alone, while GXM must be present for the vaccine to influence survival and DTH reactions. This investigation evaluated the role that major histocompatibility complex (MHC) and co-stimulatory molecules play in the non-specific and GXM-specific responses induced by GXM-APC. APC from CD40 knockout mice were as effective as wild-type APC for the induction of non-specific and GXM-specific responses. Blocking activity of B7-1 and B7-2 by treatment of immunized mice with monoclonal antibodies specific for these molecules just before and for 6 days following GXM-APC immunization decreased the splenic interferon-g response of mice subsequently infected with NU-2, but only in mice that were treated with both antibodies. These antibody treatments had no effect on DTH reactivity in similarly treated animals. MHC class I molecules were not involved in the antigen non-specific or GXM-specific activities of the vaccine. MHC class II molecules were not required for augmentation of type 1 cytokine responses but were needed for induction of the GXM-specific response that regulates the expression of DTH reactivity. This investigation has shown that an MHC class II-restricted, GXMspecific response is responsible for altering DTH responsiveness which is the correlate of immunity in this model.

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Listeria arpJGene Modifies T Helper Type 2 Subset Differentiation

et al. 2015

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A two‐step model of T cell subset commitment: antigen‐independent commitment of T cells before encountering nominal antigen during pathogenic infections

Cited by 8 publications

References 38 publications

Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells

Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells

Roles for CD40, B7 and major histocompatibility complex in induction of enhanced immunity by cryptococcal polysaccharide‐pulsed antigen‐presenting cells

Listeria arpJGene Modifies T Helper Type 2 Subset Differentiation

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