A two-stage electrophoretic microfluidic device for nucleic acid collection and enrichment

Qiao, Wen; Wang, Chinhua; Ding, Zengqian; Song, Junlan; Wei, Xiangdong; Lo, Yu‐Hwa

doi:10.1007/s10404-016-1743-0

Cited by 10 publications

(5 citation statements)

References 40 publications

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“…To overcome these limitations, researchers have introduced another layer of complexity via sheath flow, 36 coflow, 37 viscoelastic fluids, 38 and active techniques which rely upon external forces, such as electrophoresis, 39,40 magnetophoresis, 41 and acoustophoresis. 42 While this next layer of complexity has shown potential, we propose that complex geometries containing simple, passive flow-if well understood-could represent an equally viable evolutionary pathway to achieve similar results.…”

Section: Introductionmentioning

confidence: 99%

New insights into the physics of inertial microfluidics in curved microchannels. II. Adding an additive rule to understand complex cross-sections

et al. 2019

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Curved microchannels allow controllable microparticle focusing, but a full understanding of particle behavior has been limited-even for simple rectangular and trapezoidal shapes. At present, most microfluidic particle separation literature is dedicated to adding "internal" complexity (via sheath flow or obstructions) to relatively simple cross-sectional channel shapes. We propose that, with sufficient understanding of particle behavior, an equally viable pathway for microparticle focusing could utilize complex "external" cross-sectional shapes. By investigating three novel, complex spiral microchannels, we have found that it is possible to passively focus (6, 10, and 13 μm) microparticles in the middle of a convex channel. Also, we found that in concave and jagged channel designs, it is possible to create multiple, tight focusing bands. In addition to these performance benefits, we report an "additive rule" herein, which states that complex channels can be considered as multiple, independent, simple cross-sectional shapes. We show with experimental and numerical analysis that this new additive rule can accurately predict particle behavior in complex cross-sectional shaped channels and that it can help to extract general inertial focusing tendencies for suspended particles in curved channels. Overall, this work provides simple, yet reliable, guidelines for the design of advanced curved microchannel cross sections.

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Section: Introductionmentioning

confidence: 99%

New insights into the physics of inertial microfluidics in curved microchannels. II. Adding an additive rule to understand complex cross-sections

et al. 2019

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“…Capture efficiency up to 99.7% was achieved by Lo's group . The two‐stage device contained 48 cm long spiral channel and could purify 1 mL of DNA sample with 57‐fold enrichment in the first step.…”

Section: Nucleic Acids Itp On Miniaturized Systemsmentioning

confidence: 99%

Recent progress in nucleic acids isotachophoresis

Datinská

Voráčová

Schlecht³

et al. 2017

J of Separation Science

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Progress achieved between 2014–2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.

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“…The device operation was a two-step scheme with a capturing step at lower electric field and an electric lysis step at high electric field. Qiao et al 15 developed a two-stage electrophoretic device for DNA capture and enrichment with an enrichment factor of 2790. Madiyar et al 16 proposed a dielectrophoresis (DEP) device based on nanoelectrode arrays of vertically aligned carbon nanofibers for capture and detection of microbes such as bacteria and viruses with a limit of detection of 1-10 cfu ml −1 for viruses.…”

Section: Introductionmentioning

confidence: 99%

A nanofilter for fluidic devices by pillar-assisted self-assembly microparticles

2018

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We present a nanofilter based on pillar-assisted self-assembly microparticles for efficient capture of bacteria. Under an optimized condition, we simply fill the arrays of microscale pillars with submicron scale polystyrene particles to create a filter with nanoscale pore diameter in the range of 308 nm. The design parameters such as the pillar diameter and the inter-pillar spacing in the range of 5 μm-40 μm are optimized using a multi-physics finite element analysis and computational study based on bi-directionally coupled laminar flow and particle tracking solvers. The underlying dynamics of microparticles accumulation in the pillar array region are thoroughly investigated by studying the pillar wall shear stress and the filter pore diameter. The impact of design parameters on the device characteristics such as microparticles entrapment efficiency, pressure drop, and inter-pillar flow velocity is studied. We confirm a bell-curve trend in the capture efficiency versus inter-pillar spacing. Accordingly, the 10 μm inter-pillar spacing offers the highest capture capability (58.8%), with a decreasing entrapping trend for devices with larger inter-pillar spacing. This is the case that the 5 μm inter-pillar spacing demonstrates the highest pillar wall shear stress limiting its entrapping efficiency. As a proof of concept, fluorescently labeled Escherichia coli bacteria (E. coli) were captured using the proposed device. This device provides a simple design, robust operation, and ease of use. All of which are essential attributes for point of care devices.

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A two-stage electrophoretic microfluidic device for nucleic acid collection and enrichment

Cited by 10 publications

References 40 publications

New insights into the physics of inertial microfluidics in curved microchannels. II. Adding an additive rule to understand complex cross-sections

New insights into the physics of inertial microfluidics in curved microchannels. II. Adding an additive rule to understand complex cross-sections

Recent progress in nucleic acids isotachophoresis

A nanofilter for fluidic devices by pillar-assisted self-assembly microparticles

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