“…However, removal of toxic substances from hydrolyzates is an expensive process; the alternative could be development of mixed consortia which could tolerate these toxic substances. A similar effect was also observed in a study using rice straw bioethanol residues for fermentative hydrogen production [29]. There is a contradiction in the relationship between B/A ratio and the corresponding hydrogen yield, since the basic conversion reactions of butyrate formation reveals that hydrogen is utilized during the butyrate formation, and it is not a favorable one.…”
Section: Effect Of Various Pretreatment Methods On Hydrogen Productionsupporting
“…However, removal of toxic substances from hydrolyzates is an expensive process; the alternative could be development of mixed consortia which could tolerate these toxic substances. A similar effect was also observed in a study using rice straw bioethanol residues for fermentative hydrogen production [29]. There is a contradiction in the relationship between B/A ratio and the corresponding hydrogen yield, since the basic conversion reactions of butyrate formation reveals that hydrogen is utilized during the butyrate formation, and it is not a favorable one.…”
Section: Effect Of Various Pretreatment Methods On Hydrogen Productionsupporting
“…Generally, these two species are reported as potential hydrogen producers with the formation of butyrate and acetate as the major excreting metabolic product during the hydrogen fermentation and provided the stable hydrogen turnouts in the optimal pH range of 5.5e6.0 [38e40]. Besides, it has been reported that homoacetogenesis would also reduce the hydrogen yield, though the acetate production is high, additionally, B/A ratio attributes towards the higher production performances [27,41,42] (Fig. 7).…”
Section: Microbial Insights Via 454 Pyrosequencingmentioning
“…The medium for Clostridium species was used for pre-culture growth and batch experiment (Whang et al, 2011;Cheng et al, 2012c). Each liter of the medium was supplemented with the following chemicals (in mg): Sodium ammonia (400 mg-N/L) and peptone (360 mg/L) were added as nitrogen source, and trace amount of resazurin (0.175 mg/L) was also added as the redox-status indicator.…”
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