A two-stage biological gas to liquid transfer process to convert carbon dioxide into bioplastic

Rowaihi, Israa S. Al; Kick, Benjamin; Grötzinger, Stefan W.; Burger, Christian; Karan, Ram; Weuster‐Botz, Dirk; Eppinger, Jörg; Arold, Stefan T.

doi:10.1016/j.biteb.2018.02.007

Cited by 22 publications

(14 citation statements)

References 34 publications

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“…We discussed above how a twostep catalytic process can efficiently produce compounds whose direct electrochemical synthesis is inefficient, e.g., methanol. From a biological perspective, acetogenic growth on H2/CO/formate under anaerobic conditions can be coupled with subsequent aerobic microbial growth on acetate, for the production of a wide scope of chemicals, as demonstrated by several studies [86][87][88] . As many biotechnological model microbes can natively grow on acetate, coupling acetate bioproduction with its bio-consumption could prove to be a useful electromicrobial production approach.…”

Section: Discussionmentioning

confidence: 99%

Making quantitative sense of electromicrobial production

et al. 2019

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The integration of electrochemical and microbial processes offers a unique opportunity to displace fossil carbon with CO2 and renewable energy as the primary feedstocks for carbon-based chemicals. Yet, it is unclear which strategy for CO2 activation and electron transfer to microbes has the capacity to transform the chemical industry. Here, we systematically survey experimental data for microbial growth on compounds that can be produced electrochemically, either directly or indirectly. We show that only a few strategies can support efficient electromicrobial production, where formate and methanol seem the best electron mediators in terms of energetic efficiency of feedstock bio-conversion under both anaerobic and aerobic conditions. We further show that direct attachment of microbes to the cathode is highly constrained due to an inherent discrepancy between the rates of the electrochemical and biological processes. Our quantitative perspective provides a data-driven roadmap towards economically and environmentally viable realization of electromicrobial production.

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Section: Discussionmentioning

confidence: 99%

Making quantitative sense of electromicrobial production

et al. 2019

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“…The mixture was vortexed for 1 min, and the phases were separated by centrifugation (Eppendorf centrifuge 5430 R, Eppendorf, Hamburg, Germany) at 4,500 × g for 5 min at 4°C. The organic phase was collected, neutralized with 0.25 g (to 2 mL) NaHCO 3 and dried over with 0.25 g Na 2 SO 4 [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…The injection volume was 1 μL. The flow rate was 1.7 mL min −1 [ 18 ]. The measurements were performed in duplicates.…”

Section: Methodsmentioning

confidence: 99%

Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

et al. 2018

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Poly(3-hydroxybutyrate) (PHB), a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA) that mostly accumulates as a granule in the cytoplasm of microorganisms to store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup in which carbon dioxide is reduced to formate electrochemically, followed by sequential microbial conversion into PHB, using the two model strains, Methylobacterium extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall PHB production efficiency was analyzed in reasonably short reaction cycles typically as short as 8 h. As a result, the PHB formation was detected with C. necator H16 as a biocatalyst only when the electrolysis was operated in the same solution. The specificity of the source of PHB production is discussed, such as salinity, electricity, concurrent hydrogen production, and the possible involvement of reactive oxygen species (ROS).

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“…A further critical point of sequential C1 fermentations is the overall H 2 (or electron) efficiency, i.e., the efficiency of H 2 utilization for the production of a desired product. Of our examples for sequential C1 fermentation, only Hu et al (2016) reported that the overall energetic efficiency of their integrated system (from H 2 to lipid and yeast) was significantly lower than theoretically possible (see above) and Al Rowaihi et al (2018) showed energy efficiencies of 5%-55% for only the first fermentation (H 2 to acetate), depending on gas pressure and the medium used. Thus, the situation with regard to the energy requirement for sequential C1 fermentation and the biotechnological utilization of C1 substrates is unclear and remains to be examined.…”

Section: Conclusion and Prospectsmentioning

confidence: 90%

“…Starting with an optimized and waste-reduced syngas fermentation, Al Rowaihi et al (2018) reported on a two-stage Bio-GTL process to convert CO 2 into the bioplastic polyhydroxybutyrate (PHB). The authors used the pH-adjusted acetate-containing broth from an anaerobic Acetobacterium woodii-culture in a second fermentation for the synthesis of PHB under aerobic conditions, using Ralstonia eutropha H16 (nowadays designated as Cupriavidus necator H16).…”

Section: Sequential C1 Fermentationsmentioning

confidence: 99%

The Potential of Sequential Fermentations in Converting C1 Substrates to Higher-Value Products

et al. 2022

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Today production of (bulk) chemicals and fuels almost exclusively relies on petroleum-based sources, which are connected to greenhouse gas release, fueling climate change. This increases the urgence to develop alternative bio-based technologies and processes. Gaseous and liquid C1 compounds are available at low cost and often occur as waste streams. Acetogenic bacteria can directly use C1 compounds like CO, CO2, formate or methanol anaerobically, converting them into acetate and ethanol for higher-value biotechnological products. However, these microorganisms possess strict energetic limitations, which in turn pose limitations to their potential for biotechnological applications. Moreover, efficient genetic tools for strain improvement are often missing. However, focusing on the metabolic abilities acetogens provide, they can prodigiously ease these technological disadvantages. Producing acetate and ethanol from C1 compounds can fuel via bio-based intermediates conversion into more energy-demanding, higher-value products, by deploying aerobic organisms that are able to grow with acetate/ethanol as carbon and energy source. Promising new approaches have become available combining these two fermentation steps in sequential approaches, either as separate fermentations or as integrated two-stage fermentation processes. This review aims at introducing, comparing, and evaluating the published approaches of sequential C1 fermentations, delivering a list of promising organisms for the individual fermentation steps and giving an overview of the existing broad spectrum of products based on acetate and ethanol. Understanding of these pioneering approaches allows collecting ideas for new products and may open avenues toward making full use of the technological potential of these concepts for establishment of a sustainable biotechnology.

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A two-stage biological gas to liquid transfer process to convert carbon dioxide into bioplastic

Cited by 22 publications

References 34 publications

Making quantitative sense of electromicrobial production

Making quantitative sense of electromicrobial production

Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

The Potential of Sequential Fermentations in Converting C1 Substrates to Higher-Value Products

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