A two-photon ratiometric fluorescent probe for real-time imaging and quantification of NO in neural stem cells during activation regulation

Liang, Mengyu; Liu, Zhichao; Zhang, Zhonghui; Mei, Yuxiao; Tian, Yang

doi:10.1039/d2sc00326k

Cited by 22 publications

(9 citation statements)

References 45 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…48,49 The sharp peaks at 1684 cm À1 and 1490 cm À1 are in accord with the CQO stretching and N-H bending. 50 However, the FT-IR spectrum also confirmed that the Q[6]-CDs contains functional groups of ÀOH (3092 cm À1 ) and CQO (1705 cm À1 ), 51 and -C-N (1400 cm À1 ), -C-O and -C-O-C (1000-1200 cm À1 ), 52 -COOH (1050 cm À1 ) were present. New absorption bands belonging to the stretching vibration of -C-H (2867 cm À1 , 2951 cm À1 ) in the Q[6]-CDs, 53 and the stretching vibrations of C-F bond at 1249 cm À1 were observed 54 (Fig.…”

Section: Characterization Of Q[6]-cdsmentioning

confidence: 91%

Cucurbit[6]uril-based carbon dots for recognizing l-tryptophan and capecitabine

Liu

Cen

et al. 2022

Mater. Chem. Front.

View full text Add to dashboard Cite

show abstract

Section: Characterization Of Q[6]-cdsmentioning

confidence: 91%

Cucurbit[6]uril-based carbon dots for recognizing l-tryptophan and capecitabine

Liu

Cen

et al. 2022

Mater. Chem. Front.

View full text Add to dashboard Cite

show abstract

“…Some reactive small molecules like nitrogen monoxide (NO) still need a special strategy (e.g., detect NO 2 À , NO 3 À instead of NO) or use small molecule-based probes. [312][313][314][315] Normally, the DNA sequences encoding these sensors are programmed onto the sequence of adeno-associated virus (AAV), transfected into cells for screening and optimisation, and injected into animal brains several weeks before imaging. Although the transmembrane topology may make the fusion protein difficult to travel to the proper location, the high selectivity, affinity, and rich natural diversity give rise to the design of sensors of highly specific properties and applications.…”

Section: Neurotransmitter Sensorsmentioning

confidence: 99%

Fluorescent proteins and genetically encoded biosensors

Wang

Tian

2023

Chem. Soc. Rev.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fluorescence imaging has fascinated increasing regard in the field of brain imaging due to its advantages of non-radiotoxicity, non-invasiveness, high spatiotemporal resolution, rapid detection and high sensitivity [365][366][367][368][369]. With the mastery of the synthesis of fluorescent reagents and the vigorous expansion of optical technologies, fluorescence imaging technology has developed dramatically, realizing the identification and detection of brain regions [370][371][372][373][374]. The imaging resolution can reach single nanometer level, and the imaging depth in several millimeters can even be achieved.…”

Section: The Development Of Multiple Chemical Fluorescent and Raman M...mentioning

confidence: 99%

Structural and functional imaging of brains

Liu

Zhu²,

Zhang³

et al. 2022

Sci. China Chem.

Self Cite

View full text Add to dashboard Cite

Analyzing the complex structures and functions of brain is the key issue to understanding the physiological and pathological processes. Although neuronal morphology and local distribution of neurons/blood vessels in the brain have been known, the subcellular structures of cells remain challenging, especially in the live brain. In addition, the complicated brain functions involve numerous functional molecules, but the concentrations, distributions and interactions of these molecules in the brain are still poorly understood. In this review, frontier techniques available for multiscale structure imaging from organelles to the whole brain are first overviewed, including magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), serial-section electron microscopy (ssEM), light microscopy (LM) and synchrotron-based X-ray microscopy (XRM). Specially, XRM for three-dimensional (3D) imaging of large-scale brain tissue with high resolution and fast imaging speed is highlighted. Additionally, the development of elegant methods for acquisition of brain functions from electrical/chemical signals in the brain is outlined. In particular, the new electrophysiology technologies for neural recordings at the single-neuron level and in the brain are also summarized. We also focus on the construction of electrochemical probes based on dual-recognition strategy and surface/interface chemistry for determination of chemical species in the brain with high selectivity and long-term stability, as well as electrochemophysiological microarray for simultaneously recording of electrochemical and electrophysiological signals in the brain. Moreover, the recent development of brain MRI probes with high contrast-to-noise ratio (CNR) and sensitivity based on hyperpolarized techniques and multi-nuclear chemistry is introduced. Furthermore, multiple optical probes and instruments, especially the optophysiological Raman probes and fiber Raman photometry, for imaging and biosensing in live brain are emphasized. Finally, a brief perspective on existing challenges and further research development is provided.

show abstract

A two-photon ratiometric fluorescent probe for real-time imaging and quantification of NO in neural stem cells during activation regulation

Abstract: Developing a novel tool capable of real-time monitoring and accurate quantification of NO is critical to understanding its role in physiological and pathological processes. Herein, a two-photon ratiometric fluorescent probe...

Cited by 22 publications

References 45 publications

Cucurbit[6]uril-based carbon dots for recognizing l-tryptophan and capecitabine

Cucurbit[6]uril-based carbon dots for recognizing l-tryptophan and capecitabine

Fluorescent proteins and genetically encoded biosensors

Structural and functional imaging of brains

Contact Info

Product

Resources

About