A Two-Gene Balance Regulates Salmonella Typhimurium Tolerance in the Nematode Caenorhabditis elegans

Marsh, Elizabeth K.; Berg, Maaike C. W. van den; May, Robin C.

doi:10.1371/journal.pone.0016839

Cited by 29 publications

(21 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Knockdown of lys-7 also caused enhanced susceptibility toward P. aeruginosa and, interestingly, this pathogen suppresses the expression of lys-7 as part of its pathogenicity strategy 28 . In contrast, more resistance toward S. Typhimurium was observed in a lys-7 mutant, which was also shown to be more susceptible to infection with the fungus Cryptococcus neoformans 43 . Furthermore, knockdown of lys-2 , lys-5 and lys-7 led to decreased resistance against B. thuringiensis , whereas overexpression of lys-5 and lys-7 , but not lys-2 , resulted in an increase in resistance 18 .…”

Section: Lysozymesmentioning

confidence: 97%

Strength in numbers

et al. 2012

View full text Add to dashboard Cite

For more than ten years the nematode Caenorhabditis elegans has proven to be a valuable model for studies of the host response to various bacterial and fungal pathogens. When exposed to a pathogenic organism, a clear response is elicited in the nematode, which is characterized by specific alterations on the transcriptional and translational levels. Early on, researchers took advantage of the possibility to conduct large-scale investigations of the C. elegans immune response. Multiple studies demonstrated that C. elegans does indeed mount a protective response against invading pathogens, thus rendering this small nematode a very useful and simple host model for the study of innate immunity and host-pathogen interactions. Here, we provide an overview of key aspects of innate immunity in C. elegans revealed by recent whole-genome transcriptomics and proteomics studies of the global response of C. elegans to various bacterial and fungal pathogens.

show abstract

Section: Lysozymesmentioning

confidence: 97%

Strength in numbers

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Although C. elegans has no cell-mediated immunity, work by a number of groups has revealed a complex innate immune approach for disease resistance comprising avoidance behaviors (58,60) and physical barriers (25). For systemic immunity, the animal is believed to depend purely upon the secretion and action of antimicrobial molecules, including lectins (43,54,66,81), lysozymes (6,17,27,43,44,50,54,67,77,81), and antibacterial factors (34,62). Both lines of defense have been shown to be regulated by a number of signaling pathways, of which the p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases (MAPKs), insulin signaling/DAF-2, and transforming growth factor ␤ (TGF-␤)/ DBL-1 pathways are the most significant (see reference 30 for a recent review).…”

Section: Caenorhabditis Elegans and Immunitymentioning

confidence: 99%

“…Interestingly, recent work from our laboratory identified a complex genetic trade-off in the worm immune system, such that changes that increased susceptibility to killing by C. neoformans through the loss of the immune genes lys-7 and abl-1 simultaneously enhanced tolerance to S. Typhimurium, suggesting that some aspects of C. elegans immunity may provide specialized and opposing antimicrobial activities (44).…”

Section: Cryptococcus Neoformansmentioning

confidence: 99%

Caenorhabditis elegans, a Model Organism for Investigating Immunity

Marsh

May

2012

Appl Environ Microbiol

Self Cite

153

121

View full text Add to dashboard Cite

The nematode Caenorhabditis elegans has been a powerful experimental organism for almost half a century. Over the past 10 years, researchers have begun to exploit the power of C. elegans to investigate the biology of a number of human pathogens. This work has uncovered mechanisms of host immunity and pathogen virulence that are analogous to those involved during pathogenesis in humans or other animal hosts, as well as novel immunity mechanisms which appear to be unique to the worm. More recently, these investigations have uncovered details of the natural pathogens of C. elegans, including the description of a novel intracellular microsporidian parasite as well as new nodaviruses, the first identification of viral infections of this nematode. In this review, we consider the application of C. elegans to human infectious disease research, as well as consider the nematode response to these natural pathogens.

show abstract

“…Deletion of the protist type lysozyme LYS-7 can make C. elegans susceptible to killing by fungus Cryptococcus neoformans , a fatal human pathogen, but enhance its tolerance to the enteric bacteria Salmonella Typhimurium . These compound responses indicate higher levels of complexity in the C. elegans innate immune system [42]. C. elegans can respond to pathogens by generating reactive oxygen species (ROS) in the intestine while synchronically instigating an oxidative stress response which is dependent on transcriptional regulator DAF-16 to protect adjacent tissues [43].…”

Section: Interactions Between Microbial Pathogens and C Elegansmentioning

confidence: 99%

Host-Microbe Interactions inCaenorhabditis elegans

Zhang

Hou

2013

ISRN Microbiology

View full text Add to dashboard Cite

A good understanding of how microbes interact with hosts has a direct bearing on our capability of fighting infectious microbial pathogens and making good use of beneficial ones. Among the model organisms used to study reciprocal actions among microbes and hosts, C. elegans may be the most advantageous in the context of its unique attributes such as the short life cycle, easiness of laboratory maintenance, and the availability of different genetic mutants. This review summarizes the recent advances in understanding host-microbe interactions in C. elegans. Although these investigations have greatly enhanced our understanding of C. elegans-microbe relationships, all but one of them involve only one or few microbial species. We argue here that more research is needed for exploring the evolution and establishment of a complex microbial community in the worm's intestine and its interaction with the host.

show abstract

A Two-Gene Balance Regulates Salmonella Typhimurium Tolerance in the Nematode Caenorhabditis elegans

Cited by 29 publications

References 30 publications

Strength in numbers

Strength in numbers

Caenorhabditis elegans, a Model Organism for Investigating Immunity

Host-Microbe Interactions inCaenorhabditis elegans

Contact Info

Product

Resources

About