A two-dimensional thin Co-MOF nanosheet as a nanozyme with high oxidase-like activity for GSH detection

Abstract

Nanozymes with advantages of excellent enzyme mimic activity, low cost and high stability have displayed great application potential in diverse fields replacing the natural enzymes. In this study, a new...

Mixed-Valence V2O5-(Ce3+/Ce4+) Metal–Organic-Framework Nanorods for the Colorimetric and Fluorometric Detection of Glutathione

“…The K m value of nanozymes with a high affinity for a substrate is usually low. In addition, the catalytic constant K cat and the K cat / K m ratio are also the catalytic efficiencies of the enzyme, the higher these two are, the better the enzyme reaction kinetics . The K m , V max , K cat , and K cat / K m ratio of the V 2 O 5 -(Ce 3+ /Ce 4+ )-MOF using TMB as a substrate obtained 0.35 mM, 8.89 × 10 –7 M s –1 , 0.0120 s –1 , and 34.4 M –1 s –1 , respectively, by eqs and : …”

“…The K m value of nanozymes with a high affinity for a substrate is usually low. In addition, the catalytic constant K cat and the K cat / K m ratio are also the catalytic efficiencies of the enzyme, the higher these two are, the better the enzyme reaction kinetics . The K m , V max , K cat , and K cat / K m ratio of the V 2 O 5 -(Ce 3+ /Ce 4+ )-MOF using TMB as a substrate obtained 0.35 mM, 8.89 × 10 –7 M s –1 , 0.0120 s –1 , and 34.4 M –1 s –1 , respectively, by eqs and : Michaelis − Menten : V = ( V max × false[ S false] ) / ( K m + false[ S false] ) Lineweaver − Burk : 1 / V = ( false( K normalm / V max false) × false[ S false] ) + ( 1 / V max ) …”

“…In addition, the catalytic constant K cat and the K cat /K m ratio are also the catalytic efficiencies of the enzyme, the higher these two are, the better the enzyme reaction kinetics. 59 The K m , V max , K cat , and K cat /K m ratio of the V 2 O 5 -(Ce 3+ /Ce 4+ )-MOF using TMB as a substrate obtained 0.35 mM, 8.89 × 10 −7 M s −1 , 0.0120 s −1 , and 34.4 M −1 s −1 , respectively, by eqs 1 and 2: 6) reported in Table S1, the K m value of V-(Ce 3+ /Ce 4+ )-MOF is lower than that of other oxidase-like nanozymes, implying that less TMB was required to attain maximal catalytic activity for the V 2 O 5 -(Ce 3+ /Ce 4+ )-MOF. The low K m value, together with the Ce(III)/Ce(IV) balance and the adsorption nature of the MOF structure, may lead to V 2 O 5 -(Ce 3+ /Ce 4+ )-MOF's binding affinity for a catalytic substrate.…”

Reasonable construction of a bimetallic organic framework MIL-88B (Fe, Ni) nanoenzyme based on deep learning assisted doxycycline hydrochloride and methyloxytetracycline hydrochloride

“…In this context, MOFs offer a wide range of possible applications, including catalysis, gas adsorption/separation, biosensing, drug administration, and biomedical imaging. 19,20 MOFs are currently being used in the biosensors industry, such as Tb-MOF luminescent nanoenzyme catalyzed tyrosine detection, 21 two-dimensional thin Co MOF nanoenzyme for GSH detection, etc., 22 based on their high enzyme activity. MOFs can be modified by introducing acceptable metal ions, replacing them with appropriate functional groups, or combining them with other suitable materials.…”

Oxidase-like Nanozyme Activity of Manganese Metal–Organic Framework Nanosheets for Colorimetric and Fluorescence Sensing of l-Cysteine

“…For example, Dong and co-workers successfully demonstrated the use of MOF-818 with a trinuclear Cu-center for the selective oxidation of catechol to o -quinone . Further, ZIF-67, , Cu-MOFs, , and Ce-MOFs , have also been reported to show oxidase-like catalytic activity. In these MOFs, the ligands can stabilize the metal mixed-valence state to offer an efficient nanozyme activity.…”