A Two-Component Signal Transduction System Essential for Growth of Bacillus subtilis: Implications for Anti-Infective Therapy

Fabret, Céline; Hoch, James A.

doi:10.1128/.180.23.6375-6383.1998

Cited by 101 publications

(156 citation statements)

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“…Moreover, homology searches, revealed high homologies between Err07 and the YycF protein of Bacillus subtilis and Staphylococcus aureus (69% and 63% identity, respectively) and a similar domain architecture for Ehk07 and B. subtilis and S. aureus YycG HK proteins (data not shown). Thus, the Err07-Ehk07 system probably correspond to the YycFG TCS system shown to be essential in B. subtilis and S. aureus (Fabret and Hoch, 1998;Martin et al, 1999).…”

Section: Construction Of E Faecalis Jh2-2 Rr Mutantsmentioning

confidence: 98%

Molecular characterization of Enterococcus faecalis two‐component signal transduction pathways related to environmental stresses

Breton¹,

Boël²,

Benachour³

et al. 2003

Environmental Microbiology

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A key issue in the comprehension of the Enterococcus faecalis stress response is to understand how this bacterium is able to modulate its gene expression in accordance to environmental conditions. Through bioinformatic analysis of the E. faecalis V583 genome, nine two-component systems and a single orphan response regulator were identified. A transcriptional study gave evidence of four systems whose expression is induced by at least one environmental stress. In addition, gene disruption experiments allowed the isolation of eight response regulator mutants. Insertional inactivation of the response regulator gene err 05 resulted in growth default and cell morphology alterations; and also in expression default of the sagA gene, this latter being recently shown involved in E. faecalis stress resistance toward numerous lethal treatments (Y. Le Breton, A. Mazé, A. Hartke, S. Lemarinier, Y. Auffray and A. Rincé (2002) Current Microbiol 45: 434-439). Disruption of the err 04, err 08 or err 18 genes resulted in sensitivity towards heat. Finally, the err 10 mutant was shown to be more sensitive to acid pH and NaCl whereas its growth was less affected by bile salts or heat. We also demonstrated that the heat resistance phenotype of the err 10 mutant was correlated with an increase of the heat shock proteins DnaK and GroEL level.

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Section: Construction Of E Faecalis Jh2-2 Rr Mutantsmentioning

confidence: 98%

Molecular characterization of Enterococcus faecalis two‐component signal transduction pathways related to environmental stresses

Breton¹,

Boël²,

Benachour³

et al. 2003

Environmental Microbiology

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“…A two-component system comprised of an HK designated YycG and its cognate RR designated YycF is specifically conserved in Gram-positive bacteria with a low GC content. The YycG/YycF system is essential for growth of Bacillus subtilis, Staphylococcus aureus, and Streptococcus pneumoniae [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Response regulator YycF essential for bacterial growth: X‐ray crystal structure of the DNA‐binding domain and its PhoB‐like DNA recognition motif

et al. 2008

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A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.

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“…The YycG/YycF TCS is conserved and specific for low G+C Gram-positive bacteria such as B. subtilis, S. aureus, Streptococcus pneumoniae and Listeria monocytogenes [11,12,37,38]. This TCS has been shown to be essential in B. subtilis [39] and could not be inactivated by direct mutation in S. aureus [12], and which also occurs in S. epidermidis (this work).…”

Section: Discussionmentioning

confidence: 86%

“…Since the YycG/YycF TCS is essential for growth and survival in B. subtilis and S. aureus [11,12], its conservation in sequence and in genome organization in S. epidermidis strongly suggests that it is essential in this organism as well. To test this possibility, we explored whether the potential YycG inhibitor candidates obtained by virtual screening could inhibit bacterial growth.…”

Section: Antimicrobial Activities Of Potential Yycg Inhibitors In Vitromentioning

confidence: 99%

“…Recently, the TCSs have attracted attention due to their potential as novel antibacterial targets, especially those required for regulation of bacterial growth and virulence in pathogenic microorganisms [9,10]. One TCS, YycG/ YycF, highly conserved and specific to low G+C Grampositive bacteria has been shown to be essential for Bacillus subtilis and Staphylococcus aureus survival [11,12]. Inhibitors of the YycG HK, such as synthetic imidazole and zerumbone derivatives, or aranorosinol B, obtained by screening acetone extracts from 4000 microbes, have been documented to be effective antibacterial agents against B. subtilis [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Structure-based discovery of inhibitors of the YycG histidine kinase: New chemical leads to combat Staphylococcus epidermidis infections

et al. 2006

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Background: Coagulase-negative Staphylococcus epidermidis has become a major frequent cause of infections in relation to the use of implanted medical devices. The pathogenicity of S. epidermidis has been attributed to its capacity to form biofilms on surfaces of medical devices, which greatly increases its resistance to many conventional antibiotics and often results in chronic infection. It has an urgent need to design novel antibiotics against staphylococci infections, especially those can kill cells embedded in biofilm.

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A Two-Component Signal Transduction System Essential for Growth of Bacillus subtilis: Implications for Anti-Infective Therapy

Cited by 101 publications

References 0 publications

Molecular characterization of Enterococcus faecalis two‐component signal transduction pathways related to environmental stresses

Molecular characterization of Enterococcus faecalis two‐component signal transduction pathways related to environmental stresses

Response regulator YycF essential for bacterial growth: X‐ray crystal structure of the DNA‐binding domain and its PhoB‐like DNA recognition motif

Structure-based discovery of inhibitors of the YycG histidine kinase: New chemical leads to combat Staphylococcus epidermidis infections

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