A Two-Component Regulatory System Integrates Redox State and Population Density Sensing in
            <i>Pseudomonas putida</i>

Fernández-Piñar, Regina; Ramos, Juan L.; Rodríguez‐Herva, José J.; Espinosa‐Urgel, Manuel

doi:10.1128/jb.00868-08

Cited by 34 publications

(44 citation statements)

References 35 publications

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“…The two-component signal transduction system (TCS) is a stimulus–response coupling signal transduction machinery that allows bacteria to respond and adapt to changes in a wide range of environmental conditions, such as nutrient assimilation [32], cellular redox state [33] and bacterial virulence regulation [34]. Chemotaxis, controlled by TCS, is the cells’ response to stressful environments.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation

Zhang

Ezeji

2013

Biotechnol Biofuels

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BackgroundFurfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (ABE) producing Clostridium beijerinckii NCIMB 8052 to this compound at the molecular level is unknown. To discern the effect of furfural on C. beijerinckii and to gain insight into molecular mechanisms of action and detoxification, physiological changes of furfural-stressed cultures during acetone butanol ethanol (ABE) fermentation were studied, and differentially expressed genes were profiled by genome-wide transcriptional analysis.ResultsA total of 5,003 C. beijerinckii NCIMB 8052 genes capturing about 99.7% of the genome were examined. About 111 genes were differentially expressed (up- or down-regulated) by C. beijerinckii when it was challenged with furfural at acidogenic growth phase compared with 721 genes that were differentially expressed (up- or down-regulated) when C. beijerinckii was challenged with furfural at solventogenic growth phase. The differentially expressed genes include genes related to redox and cofactors, membrane transporters, carbohydrate, amino sugar and nucleotide sugar metabolisms, heat shock proteins, DNA repair, and two-component signal transduction system. While C. beijerinckii exposed to furfural stress during the acidogenic growth phase produced 13% more ABE than the unstressed control, ABE production by C. beijerinckii ceased following exposure to furfural stress during the solventogenic growth phase.ConclusionGenome-wide transcriptional response of C. beijerinckii to furfural stress was investigated for the first time using microarray analysis. Stresses emanating from ABE accumulation in the fermentation medium; redox balance perturbations; and repression of genes that code for the phosphotransferase system, cell motility and flagellar proteins (and combinations thereof) may have caused the premature termination of C. beijerinckii 8052 growth and ABE production following furfural challenge at the solventogenic phase.This study provides insights into basis for metabolic engineering of C. beijerinckii NCIMB 8052 for enhanced tolerance of lignocellulose-derived microbial inhibitory compounds, thereby improving bioconversion of lignocellulose biomass hydrolysates to biofuels and chemicals. Indeed, two enzymes encoded by Cbei_3974 and Cbei_3904 belonging to aldo/keto reductase (AKR) and short-chain dehydrogenase/reductase (SDR) families have been identified to be involved in furfural detoxification and tolerance.

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Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation

Zhang

Ezeji

2013

Biotechnol Biofuels

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“…The RoxSR regulon of Ps. putida includes genes for respiratory function and maintenance of the redox balance, genes involved in sugar and amino acid metabolism, and the sulfur starvation response (78). These authors also showed that RoxSR participates in cell density signal transduction in Ps.…”

Section: Pseudomonas Species Roxsrmentioning

confidence: 95%

Bacterial Adaptation of Respiration from Oxic to Microoxic and Anoxic Conditions: Redox Control

Cendejas-Bueno

Mesa

Bedmar

et al. 2012

Antioxidants & Redox Signaling

147

148

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Under a shortage of oxygen, bacterial growth can be faced mainly by two ATP-generating mechanisms: (i) by synthesis of specific high-affinity terminal oxidases that allow bacteria to use traces of oxygen or (ii) by utilizing other substrates as final electron acceptors such as nitrate, which can be reduced to dinitrogen gas through denitrification or to ammonium. This bacterial respiratory shift from oxic to microoxic and anoxic conditions requires a regulatory strategy which ensures that cells can sense and respond to changes in oxygen tension and to the availability of other electron acceptors. Bacteria can sense oxygen by direct interaction of this molecule with a membrane protein receptor (e.g., FixL) or by interaction with a cytoplasmic transcriptional factor (e.g., Fnr). A third type of oxygen perception is based on sensing changes in redox state of molecules within the cell. Redox-responsive regulatory systems (e.g., ArcBA, RegBA/PrrBA, RoxSR, RegSR, ActSR, ResDE, and Rex) integrate the response to multiple signals (e.g., ubiquinone, menaquinone, redox active cysteine, electron transport to terminal oxidases, and NAD/NADH) and activate or repress target genes to coordinate the adaptation of bacterial respiration from oxic to anoxic conditions. Here, we provide a compilation of the current knowledge about proteins and regulatory networks involved in the redox control of the respiratory adaptation of different bacterial species to microxic and anoxic environments.

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“…To test if the observed activation of ddcA expression by culture supernatants of P. aeruginosa could be mediated by PpoR, the reporter plasmid pME510 was introduced into the strain EU6, a ppoR mutant of KT2440 (Fernández-Piñar et al, 2011b) and b-galactosidase activity analysed both in liquid cultures and using the disc assay described above. In contrast, expression of the ddcA-::lacZ fusion was not activated by culture supernatants or extracts when pME510 was introduced in strain EU58, a roxS transposon insertion mutant (Fernández-Piñar et al, 2008). This indicated that the response of ddcA to interspecies signalling molecules is independent of PpoR.…”

Section: Resultsmentioning

confidence: 95%

Fatty acid‐mediated signalling between two Pseudomonas species

Fernández-Piñar

Espinosa‐Urgel

Dubern

et al. 2012

Environ Microbiol Rep

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We report the identification of fatty acids as mediators of intercellular signalling in Pseudomonas putida, and between Pseudomonas aeruginosa and P. putida. Tetradecanoic acid and fatty acids of similar chain length are present in supernatants of these strains and activate population density-dependent expression of ddcA, a gene involved in corn seed and root colonization by P. putida KT2440. Consistently, significant amounts of these compounds were also found in corn root exudates. The signalling pathway involves the two-component regulatory system formed by RoxS and RoxR, which had been previously shown to control expression of ddcA and of a set of genes related to the redox balance of P. putida cells. Production of the fatty acid signal in P. aeruginosa is under the control of the LasI/LasR and RhlI/RhlR quorum sensing systems. Our data indicate that in terms of cell-cell communication, P. putida KT2440 employs mechanisms closer to those of plant pathogens such as Xanthomonas spp. and fungi like Candida, which also rely on fatty acid derivatives.

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A Two-Component Regulatory System Integrates Redox State and Population Density Sensing in Pseudomonas putida

Cited by 34 publications

References 35 publications

Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation

Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation

Bacterial Adaptation of Respiration from Oxic to Microoxic and Anoxic Conditions: Redox Control

Fatty acid‐mediated signalling between two Pseudomonas species

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