A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics
Zicong Wang,
Peng-Kai Liu,
Lingjun Li
Abstract:Recent advancements in mass spectrometry (MS) have revolutionized
quantitative proteomics, with multiplex isotope labeling emerging
as a key strategy for enhancing accuracy, precision, and throughput.
This tutorial review offers a comprehensive overview of multiplex
isotope labeling techniques, including precursor-based, mass defect-based,
reporter ion-based, and hybrid labeling methods. It details their
fundamental principles, advantages, and inherent limitations along
with strategies to mitigate the limitati… Show more
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