A “turn on/off” scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy

Abstract

A "turn on/off" biosensor for diagnosis of exon 7 of the SMN1 gene was developed by employing a "scorpion primer". This scorpion primer was based on the principle of fluorescence resonance energy transfer using a fluorophore, a blocker and a quencher. It was successfully applied to detect 10 volunteer samples, and not only to in vitro testing.

Copper nanoclusters on specific-primer PCR fragments with magnetic capture for the label-free fluorescent sensing of the T315I single nucleotide variant in the BCR–ABL1 gene

“…In this study, capillary electrophoresis (CE) was utilized to confirm the PCR products, and compared with a DNA ladder standard. 31 As shown in Fig. 2A, both the wild and mutant DNA templates have a peak at about 12 min, corresponding to a length between 100 and 200 base pairs.…”

Fluorescent Double-Stranded DNA-Templated Copper Nanoprobes for Rapid Diagnosis of Tuberculosis

“…In DNA-based detection, fluorescent labeling is typically used to directly indicate the existence of target DNA sequences. − Recently, researchers have developed fluorescent metal nanoprobes (NPs), such as copper, − silver, − gold, , and alloy , nanoparticles/nanoclusters, for molecular diagnostics because of their unique optical, electronic, and catalytic properties. In particular, copper nanoparticle-based nanoprobes (Cu NPs) exhibit various attractive characteristics, including ease of synthesis, high water solubility, low toxicity, and excellent biocompatibility, and stability.…”