A Turn-On and Colorimetric Probe Based on Isophorone Skeleton for Detecting Nerve Agent Mimic Diethyl Chlorophosphite

Abstract

A new turn-on probe (SWJT-20) based on isophorone fluorophore for the detection of nerve agent mimic diethyl chlorophosphite (DCP) was designed and synthesized. SWJT-20 could rapidly respond to DCP within 2 s using UV-Vis or fluorescent spectra, accompanied by a significant change in the solution color under visible light or UV light, which could be observed by the naked eyes. The detection limit of SWJT-20 to DCP was as low as 8.3 nM, which is lower than those of most reported fluorescent probes for DCP detection. Additionally, SWJT-20 could quantitatively measure DCP using ratio changes in A427/A645 in absorption spectra. Furthermore, facile paper as sensors with the visualization of colorimetric/fluorometric responses based on SWJT-20 has been fabricated. Notably, this probe could detect DCP vapor through gas diffusion experiments.

A ratiometric, colorimetric fluorescent probe with a large emission shift for the rapid detection of diethyl chlorophosphate

“…In connection with our research on DCP detection, 19,44–46 herein we designed a ratiometric fluorescent probe, SWJT-16 , based on the isophorone fluorophore for the detection of DCP (Scheme 1). SWJT-16 , with a large emission shift (Δ λ em = 174 nm), was found to respond quickly to DCP, within 6 seconds, and to have a maximum emission wavelength at 728 nm, i.e.…”

A novel “on–off–on” near-infrared fluorescent probe for Cu2+ and S2− continuous detection based on dicyanoisoflurone derivatives, and its application in bacterial imaging

“…Because the synthesis method of dicyanoisourone (DCI) is simple and most of its derivatives exhibit typical near-infrared uorescence emission wavelengths, it has become one of the main uorophores for the synthesis of near-infrared uorescent probes in recent years. [29][30][31] In 2021, our research group 32 designed and synthesized a uorescent probe that can continuously identify Zn 2+ and CN − based on dicyanoisourone derivatives. The probe NIRF has high sensitivity and selectivity for Zn 2+ and CN − , when Zn 2+ is added, the uorescence intensity at 658 nm is gradually enhanced, and then CN − is added to the above reaction system, and [NIRF-Zn 2+ ] can undergo a coordination exchange reaction with CN − to form the compound [Zn(CN) x ] 1−x , and then the probe will return to its original structure, resulting in a gradual weakening of the uorescence intensity at 658 nm, leading to an "off-on-off" uorescence signal conversion.…”

“…Because the synthesis method of dicyanoisoflurone (DCI) is simple and most of its derivatives exhibit typical near-infrared fluorescence emission wavelengths, it has become one of the main fluorophores for the synthesis of near-infrared fluorescent probes in recent years. 29–31…”