A tunable filter for high molecular weight DNA selection and linked-read sequencing

Abstract

In third generation sequencing, long DNA molecules of more than ∼20 kbp are needed to obtain quality sequence data. Here we report a versatile technology for DNA size selection that fulfills this requirement.

micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation

“…S2). Notably, in comparison to a fragment of 1 kbp (Milon et al, 2020), the concentration of the miR-21C:MB complex of ~30 bp required flow velocities and electric fields enhanced by 20fold and 200-fold, respectively.…”

“…Concentration can also be performed with the µ-Laboratory for DNA analysis and separation (µLAS) technology, which is based on the combination of hydrodynamic transport and a counter electrophoretic force (Malbec et al, 2019;Milon et al, 2020;Ranchon et al, 2016).…”

Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography

“…Using conventional MB and isotachophoresis for concentration, an LoD of 5 pM in 3 minutes has been reported [20][21][22] . In line with this biosensing technology based on a concentration module, we recently developed the µLAS technology (µ-Laboratory for DNA analysis and separation), which enables DNA size separation, concentration, and purification in a microchannel using bi-directional electrohydrodynamic actuation [23][24][25][26] . This technology has been exploited to detect nucleic acids by the selective concentration of the complex formed between the target molecule and a complementary MB 27 .…”

Characterization and minimization of band broadening in DNA electrohydrodynamic migration for enhanced size separation

“…Some predictions of models have been tested experimentally and validated 20,23,24 . Furthermore, dual actuation aroused attention because it enabled the concentration of DNA in microchannels with a funnel geometry 18,19,[25][26][27] . The operations of DNA concentration and separation could be performed on-line with the so-called µLAS (µ-Laboratory for DNA Analysis and Separation) technology to produce high sensitivity DNA profiles with a limit of detection of 10 fg/µL 28,29 .…”