Abstract:Treatment of the vacuolar H+ -ATPase from mung bean
seedlings (Vigna radiata L.) with the tryptophan
modifying agent 2-hydroxyl-5-nitrobenzyl bromide (HNBB), caused a progressive
decline of the ATP hydrolysis activity and proton translocation in a time- and
concentration-dependent manner. Dithiothreitol could not restore the
inhibition of H+ -ATPase by HNBB, indicating possible
involvement tryptophan, and not cysteine residues. Protection studies
suggested that modified sites might not locate in the active dom… Show more
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