A TRP channel is expressed in <i>Spodoptera littoralis</i> antennae and is potentially involved in insect olfactory transduction

Chouquet, Bastien; Debernard, Stéphane; Bozzolan, Françoise; Solvar, Marthe; Maı̈bèche-Coisné, Martine; Lucas, Philippe

doi:10.1111/j.1365-2583.2008.00857.x

Cited by 13 publications

(13 citation statements)

References 63 publications

(141 reference statements)

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“…Among examples, we annotated unigenes putatively encoding proteins such as G-proteins and G-protein related elements, second messenger-related enzymes and ions channels such as voltage-gated ion channels, calcium and chloride channels (additional files 1 and 2). Some of these genes have been previously described in detail, such as a diacylglycerol kinase [63] and a transient receptor potential channel [64], whose function in pheromone signal transduction was suspected. However, the way insect ORs transduce the signal is currently under debate and although a classical metabotropic pathway via G-protein was assumed [65], recent studies proposed an alternative or complementary ionotropic process [66,67].…”

Section: Resultsmentioning

confidence: 99%

An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research

Legeai

Malpel

Montagné³

et al. 2011

BMC Genomics

130

162

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BackgroundNocturnal insects such as moths are ideal models to study the molecular bases of olfaction that they use, among examples, for the detection of mating partners and host plants. Knowing how an odour generates a neuronal signal in insect antennae is crucial for understanding the physiological bases of olfaction, and also could lead to the identification of original targets for the development of olfactory-based control strategies against herbivorous moth pests. Here, we describe an Expressed Sequence Tag (EST) project to characterize the antennal transcriptome of the noctuid pest model, Spodoptera littoralis, and to identify candidate genes involved in odour/pheromone detection.ResultsBy targeting cDNAs from male antennae, we biased gene discovery towards genes potentially involved in male olfaction, including pheromone reception. A total of 20760 ESTs were obtained from a normalized library and were assembled in 9033 unigenes. 6530 were annotated based on BLAST analyses and gene prediction software identified 6738 ORFs. The unigenes were compared to the Bombyx mori proteome and to ESTs derived from Lepidoptera transcriptome projects. We identified a large number of candidate genes involved in odour and pheromone detection and turnover, including 31 candidate chemosensory receptor genes, but also genes potentially involved in olfactory modulation.ConclusionsOur project has generated a large collection of antennal transcripts from a Lepidoptera. The normalization process, allowing enrichment in low abundant genes, proved to be particularly relevant to identify chemosensory receptors in a species for which no genomic data are available. Our results also suggest that olfactory modulation can take place at the level of the antennae itself. These EST resources will be invaluable for exploring the mechanisms of olfaction and pheromone detection in S. littoralis, and for ultimately identifying original targets to fight against moth herbivorous pests.

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Section: Resultsmentioning

confidence: 99%

An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research

Legeai

Malpel

Montagné³

et al. 2011

BMC Genomics

130

162

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“…In accordance, a diacylglycerol kinase (which converts DAG to phosphatidic acid and recycles phosphatidylinositols) was located in olfactory sensilla trichodea of S. litt ralis (Chouquet et al, 2008). Because IP 3 - and DAG-gated ion channels most likely belong to the TRP-superfamily of ion channels (review: Dong et al, 2010) the localization of TRP channels in moth antennae further substantiates the importance of the PLCβ-dependent signal transduction pathway in moth olfaction (Ackermann, 2004; Chouquet et al, 2009). A PLCβ-dependent cascade might be employed not only in pheromone-transduction but also in general odor transduction.…”

Section: G-protein Coupled Phospholipase Cβ-dependent Odor Transductimentioning

confidence: 95%

Pheromone Transduction in Moths

Stengl

2010

Front. Cell. Neurosci.

137

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Calling female moths attract their mates late at night with intermittent release of a species-specific sex-pheromone blend. Mean frequency of pheromone filaments encodes distance to the calling female. In their zig-zagging upwind search male moths encounter turbulent pheromone blend filaments at highly variable concentrations and frequencies. The male moth antennae are delicately designed to detect and distinguish even traces of these sex pheromones amongst the abundance of other odors. Its olfactory receptor neurons sense even single pheromone molecules and track intermittent pheromone filaments of highly variable frequencies up to about 30 Hz over a wide concentration range. In the hawkmoth Manduca sexta brief, weak pheromone stimuli as encountered during flight are detected via a metabotropic PLCβ-dependent signal transduction cascade which leads to transient changes in intracellular Ca2+ concentrations. Strong or long pheromone stimuli, which are possibly perceived in direct contact with the female, activate receptor-guanylyl cyclases causing long-term adaptation. In addition, depending on endogenous rhythms of the moth's physiological state, hormones such as the stress hormone octopamine modulate second messenger levels in sensory neurons. High octopamine levels during the activity phase maximize temporal resolution cAMP-dependently as a prerequisite to mate location. Thus, I suggest that sliding adjustment of odor response threshold and kinetics is based upon relative concentration ratios of intracellular Ca2+ and cyclic nucleotide levels which gate different ion channels synergistically. In addition, I propose a new hypothesis for the cyclic nucleotide-dependent ion channel formed by insect olfactory receptor/coreceptor complexes. Instead of being employed for an ionotropic mechanism of odor detection it is proposed to control subthreshold membrane potential oscillation of sensory neurons, as a basis for temporal encoding of odors.

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“…isothiocyanate, citronellal, menthol) emitted from plants are demonstrated to interact with antennal TRPs (Al-Anzi et al, 2006) and to repel Drosophila melanogaster (Kwon et al, 2010). In addition, recent experiments demonstrate the expression of TRP channels in the antennae of Spodoptera littoralis (Lepidoptera: Noctuidae), at the base of olfactory sensilla (Chouquet et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Response of the European grapevine moth Lobesia botrana to somatosensory‐active volatiles emitted by the non‐host plant Perilla frutescens

et al. 2014

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The European grapevine moth Lobesia botrana Denis & Schiffermüller (Lepidoptera: Tortricidae) is a major pest on grapes worldwide. Attempts to develop control methods for this pest based on grape kairomones demonstrate limited success and studies indicate that a major limiting factor is overlap between synthetic kairomones and background odours in the vineyard. Behaviourally active compounds from non‐host plants may thus represent an effective alternative for monitoring and control methods. Extracts from food plants (i.e. from capsicum, garlic and peppermint, which elicit the so‐called somatosensory sensation) are traditionally used in agriculture for the control of pest insects. Among those plants, Perilla frutescens L. (Lamiales: Lamiaceae), native of Asia, contains compounds activating sensory ion channels in mammals, which are known to be involved in the perception of somatosensory compounds and are expressed in tortricid moth antennae. In the present study, in search of non‐host volatiles with potential application in pest control, essential oil metabolites isolated from P. frutescens are screened for biological activity on the olfactory system of L. botrana. The compounds (S)‐(−)‐perillaldehyde and isoegomaketone, which are released from different P. frutescens varieties, are identified by gas chromatography‐coupled electroantennographic detection. In a dual‐choice oviposition test, females show a preference for a combination of host odours and perillaldehyde, preferring this over a host‐plant odour bouquet alone. In Y‐tube olfactometer assays, virgin males show a higher level of activity in the presence of isoegomaketone, even if not significantly responsive to the compound.

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A TRP channel is expressed in Spodoptera littoralis antennae and is potentially involved in insect olfactory transduction

Cited by 13 publications

References 63 publications

An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research

An Expressed Sequence Tag collection from the male antennae of the Noctuid moth Spodoptera littoralis: a resource for olfactory and pheromone detection research

Pheromone Transduction in Moths

Response of the European grapevine moth Lobesia botrana to somatosensory‐active volatiles emitted by the non‐host plant Perilla frutescens

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