A trophic transfer study: accumulation of multi-walled carbon nanotubes associated to green algae in water flea Daphnia magna

Politowski, Irina; Wittmers, Fabian; Hennig, Michael; Siebers, Nina; Goffart, Birgitta; Roß-Nickoll, Martina; Ottermanns, Richard

doi:10.1016/j.impact.2021.100303

Cited by 12 publications

(12 citation statements)

References 66 publications

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“…The first step in our study was to investigate possible structural differences between wMWCNTs and pristine MWCNTs (MWCNTs) and additionally the enrichment of wMWCNTs in benthic biofilm. The impact of MWCNTs on primary producers, stress phenomena, and changes in the composition of algal cells was investigated in various publications (Long et al 2012;Rhiem et al 2015;Schwab et al 2011;Wang et al 2019;Politowski et al 2021b). However, higher-tier approaches have been neglected, and the available approaches are not necessarily applicable to investigate food web interactions.…”

Section: Introductionmentioning

confidence: 99%

“…However, higher-tier approaches have been neglected, and the available approaches are not necessarily applicable to investigate food web interactions. For primary consumers, such as the water flea Daphnia magna (D. magna), it has been shown that CNTs can accumulate in their body and that depuration is delayed in the absence of food (Petersen et al 2009;Tervonen et al 2010;Politowski et al 2021b). Guo et al (2013) investigated the depuration and the uptake of radiolabeled graphene by D. magna and described the release of significant fractions (~ 90%) of accumulated graphene after the addition of algae and humic acid during a depuration period.…”

Section: Introductionmentioning

confidence: 99%

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A workflow to investigate the impacts of weathered multi-walled carbon nanotubes to the mud snail Lymnaea stagnalis

Weise

Kurth

Politowski

et al. 2021

Environ Sci Pollut Res

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Although the development and application of nanomaterials is a growing industry, little data is available on the ecotoxicological effects on aquatic organisms. Therefore, we set up a workflow to address the potential uptake of weathered multi-walled carbon nanotubes (wMWCNTs) by a model organism, the pulmonary mud snail Lymnaea stagnalis (L. stagnalis), which plays an important role in the food web. It represents a suitable organism for this approach because as a grazer it potentially ingests large amounts of sedimented wMWCNTs. As food source for L. stagnalis, benthic biofilm was investigated by the use of a transmission electron microscope (TEM) and a scanning electron microscope (SEM) after exposure with wMWCNTs. In addition, isotopic labeling was applied with 14C-wMWCNTs (0.1 mg/L) to quantify fate, behavior, and enrichment of 14C-wMWCNTs in benthic biofilm and in L. stagnalis. Enrichment in benthic biofilm amounted to 529.0 µg wMWCNTs/g dry weight and in L. stagnalis to 79.6 µg wMWCNTs/g dry weight. A bioconcentration factor (BCF) for L. stagnalis was calculated (3500 L/kg). We demonstrate the accumulation of wMWCNTs (10 mg/L) in the digestive tract of L. stagnalis in an effect study. Moreover, the physiological markers glycogen and triglycerides as indicators for the physiological state, as well as the RNA/DNA ratio as growth indicator, were examined. No significant differences between exposed and control animals were analyzed for glycogen and triglycerides after 24 days of exposure, but a decreasing trend is recognizable for triglycerides. In contrast, the significant reduction in the RNA/DNA ratio of L. stagnalis indicated an inhibition of growth with a following recovery after depuration. The described workflow enables a comprehensive determination of the fate and the behavior of wMWCNTs specifically and in general all kinds of CNTs in the aquatic environment and therefore contributes to a holistic risk assessment of wMWCNTs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A workflow to investigate the impacts of weathered multi-walled carbon nanotubes to the mud snail Lymnaea stagnalis

Weise

Kurth

Politowski

et al. 2021

Environ Sci Pollut Res

Self Cite

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“…A detailed description and characterization of MWCNTs can be found in Politowski et al ( 40 , 41 ) and Weise et al ( 27 ). Agglomerates with their typical cross-link structure and single strands of MWCNTs displaying a spiral shape with some irregularities were identified ( Fig.…”

Section: Methodsmentioning

confidence: 99%

Multiwalled Carbon Nanotubes Promote Bacterial Conjugative Plasmid Transfer

et al. 2022

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“…The rst step in our study was to investigate possible structural differences between wMWCNTs and pristine MWCNTs (MWCNTs) and additionally the enrichment of wMWCNTs in benthic bio lm. The impact of MWCNTs on primary producers, stress phenomena, and changes in the composition of algal cells were investigated in various publications (Long et al 2012;Rhiem et al 2015;Schwab et al 2011;Wang et al 2019;Politowski et al 2021b). However, higher-tier approaches have been neglected and the available approaches are not necessarily applicable to investigate food web interactions.…”

Section: Introductionmentioning

confidence: 99%

“…However, higher-tier approaches have been neglected and the available approaches are not necessarily applicable to investigate food web interactions. For primary consumers, such as the water ea Daphnia magna it has been shown that CNTs can accumulate in their body and that depuration is delayed in the absence of food (Petersen et al 2009; Tervonen et al 2010; Politowski et al 2021b). It has also been shown that CNTs from food matrix, or sediment, are accumulated in aquatic invertebrates (Li et al 2010;Wang et al 2014;Petersen et al 2008;Parks et al 2013).…”

Section: Introductionmentioning

confidence: 99%

A Workflow to Investigate the Impacts of Weathered Multi-Walled Carbon Nanotubes to the Mudsnail Lymnaea Stagnalis

Weise

Kurth

Politowski

et al. 2021

Preprint

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Although the development and application of nanomaterials is a growing industry, little data is available on the ecotoxicological effects on aquatic organisms. Therefore, we set up a workflow to address the potential uptake of weathered multi-walled carbon nanotubes (wMWCNTs) by a model organism, the pulmonary mudsnail Lymnaea stagnalis (L. stagnalis), which plays an important role in the food web. It represents a suitable organism for this approach because as a grazer it potentially ingests large amounts of sedimented wMWCNTs. As food source for L. stagnalis, benthic biofilm was investigated by the use of a transmission electron microscope (TEM), and a scanning electron microscope (SEM) after exposure with wMWCNTs. In addition, isotopic labeling was applied with 14C-wMWCNTs (0.1 mg/L) to quantify fate, behavior and enrichment of 14C-wMWCNTs in benthic biofilm and in L. stagnalis. Enrichment in benthic biofilm amounted to 529.0 µg wMWCNTs/g dry weight and in L. stagnalis to 79.6 µg wMWCNTs/g dry weight. A bioconcentration factor (BCF) for L. stagnalis was calculated (3,500 L/kg). We demonstrate the accumulation of wMWCNTs (10 mg/L) in the digestive tract of L. stagnalis in an effect study. Moreover, the physiological markers glycogen and triglycerides as indicators for the physiological state, as well as the RNA/DNA ratio as growth indicator were examined. No significant differences between exposed and control animals were analyzed for glycogen and triglycerides after 24 d of exposure, but a decreasing trend is recognizable for triglycerides. In contrast, the significant reduction in the RNA/DNA ratio of L. stagnalis indicated an inhibition of growth with a following recovery after depuration. The described workflow enables a comprehensive determination of the fate and the behavior of wMWCNTs in specifically and in general all kinds of CNT in the aquatic environment and therefore contributes to a holistic risk assessment of wMWCNTs.

show abstract

A trophic transfer study: accumulation of multi-walled carbon nanotubes associated to green algae in water flea Daphnia magna

Cited by 12 publications

References 66 publications

A workflow to investigate the impacts of weathered multi-walled carbon nanotubes to the mud snail Lymnaea stagnalis

A workflow to investigate the impacts of weathered multi-walled carbon nanotubes to the mud snail Lymnaea stagnalis

Multiwalled Carbon Nanotubes Promote Bacterial Conjugative Plasmid Transfer

A Workflow to Investigate the Impacts of Weathered Multi-Walled Carbon Nanotubes to the Mudsnail Lymnaea Stagnalis

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