A Triggered DNA Hydrogel Cover to Envelop and Release Single Cells

Jin, Juan; Xing, Yongzheng; Xi, Yanli; Liu, Xueli; Zhou, Tao; Ma, Xinxin; Yang, Zhongqiang; Wang, Shutao; Li, Dongsheng

doi:10.1002/adma.201301175

Cited by 134 publications

(133 citation statements)

References 45 publications

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“…Later, this group also generated a DNA‐single‐walled carbon nanotube hybrid hydrogel, which is pH‐responsive and strength‐tunable . Furthermore, DNA hydrogels were developed to envelop and release single cells . Thereby, the release of cells can be controlled by the specific digestion of the DNA hydrogel using restriction enzymes.…”

Section: Discussionmentioning

confidence: 99%

Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility

Stoll¹,

Steinle²,

Stang³

et al. 2016

Macromolecular Bioscience

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Hemocompatibility and cytocompatibility of biomaterials codetermine the success of tissue engineering applications. DNA, the natural component of our cells, is an auspicious biomaterial for the generation of designable scaffolds with tailorable characteristics. In this study, a combination of rolling circle amplification and multiprimed chain amplification is used to generate hydrogels at centimeter scale consisting solely of DNA. Using an in vitro rotation model and fresh human blood, the reaction of the hemostatic system on DNA hydrogels is analyzed. The measurements of hemolysis, platelets activation, and the activation of the complement, coagulation, and neutrophils using enzyme-linked immunosorbent assays demonstrate excellent hemocompatibility. In addition, the cytocompatibility of the DNA hydrogels is tested by indirect contact (agar diffusion tests) and material extract experiments with L929 murine fibroblasts according to the ISO 10993-5 specifications and no negative impact on the cell viability is detected. These results indicate the promising potential of DNA hydrogels as biomaterials for versatile applications in the field of regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility

Stoll¹,

Steinle²,

Stang³

et al. 2016

Macromolecular Bioscience

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“…23–25 Moreover, DNA can be utilized to construct structure-switchable nanomachines that respond to external stimuli such as temperature, light, pH and small molecules. 26–29 For instance, an artificial DNA nano-spring powered by protons has been constructed by assembling multiple oligonucleotides and used for controlling the distance between gold nanoparticles. 30,31 However, the driving force of the developed DNA nano-spring is protons, which severely hindered its application for cell culture where a neutral pH is needed.…”

Section: Introductionmentioning

confidence: 99%

Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands

et al. 2017

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“…This type of hydrogel has been used to trap single living cells in microwells. [6] While in previous approaches branched DNA motifs were required for the formation of DNA hydrogels, [1][2][3][4][5][6] we generated a DNA hydrogel solely from linear dsDNA equipped with sticky ends. A combined experimental study on the selfassembly of linear dsDNA building blocks using diffusionordered NMR spectroscopy (DOSY NMR), rheology, and atomic force microscopy (AFM) is presented.…”

mentioning

confidence: 99%

Construction of Three‐Dimensional DNA Hydrogels from Linear Building Blocks

et al. 2014

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A three-dimensional DNA hydrogel was generated by self-assembly of short linear double-stranded DNA (dsDNA) building blocks equipped with sticky ends. The resulting DNA hydrogel is thermoresponsive and the length of the supramolecular dsDNA structures varies with temperature. The average diffusion coefficients of the supramolecular dsDNA structures formed by self-assembly were determined by diffusion-ordered NMR spectroscopy (DOSY NMR) for temperatures higher than 60 8C. Temperature-dependent rheological measurements revealed a gel point of 42 AE 1 8C. Below this temperature, the resulting material behaved as a true gel of high viscosity with values for the storage modulus G' being significantly larger than that for the loss modulus G''. Frequency-dependent rheological measurements at 20 8C revealed a mesh size (x) of 15 nm. AFM analysis of the diluted hydrogel in the dry state showed densely packed structures of entangled chains, which are also expected to contain multiple interlocked rings and catenanes.Since the first report on the formation of a pristine DNA hydrogel in 2006, [1,2] there is growing interest in the development of DNA hydrogels especially for applications as drugrelease systems. [2][3][4] When DNA-hydrogels were generated from flexible branched double-stranded DNA (dsDNA) building blocks which were linked covalently, the enzymecatalyzed gel formation and the release of entrapped molecules were rather slow. [2,5] As an alternative, a fastresponding pH-triggered DNA hydrogel has been formed from Y-shaped DNA building blocks, which were equipped with cytosine-rich interlocking i-motif domains.[3] At low pH values, a hydrogel was obtained, which was able to trap gold nanoparticles (AuNPs). When the pH value was changed to pH 8, the gel quickly disassembled and the AuNPs were released.[3]More recently, DNA hydrogels have been formed by hybridization of Y-scaffolds with linear (linker) dsDNA. [4] Hydrogel formation could be reversed by heating above the melting temperature, and the temperature-dependent gel-sol transition could be monitored by rheological measurements. This type of hydrogel has been used to trap single living cells in microwells. [6] While in previous approaches branched DNA motifs were required for the formation of DNA hydrogels, [1][2][3][4][5][6] we generated a DNA hydrogel solely from linear dsDNA equipped with sticky ends. A combined experimental study on the selfassembly of linear dsDNA building blocks using diffusionordered NMR spectroscopy (DOSY NMR), rheology, and atomic force microscopy (AFM) is presented.The sequences of the investigated single-stranded DNA (ssDNA) and dsDNA monomers are shown in Scheme 1. By hybridization of two oligomers, oligo 1 (O1) with oligo 2 (O2), a monomeric dsDNA building block O1-O2 with 30 base pairs (bp) is formed. This building block is further equipped with two complementary overhangs (sticky ends) of 15 bases for self-assembly. As a reference compound, a monomeric dsDNA building block O1-O3 (30 bp) with two noncomplementary overha...

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A Triggered DNA Hydrogel Cover to Envelop and Release Single Cells

Cited by 134 publications

References 45 publications

Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility

Generation of Large‐Scale DNA Hydrogels with Excellent Blood and Cell Compatibility

Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands

Construction of Three‐Dimensional DNA Hydrogels from Linear Building Blocks

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