A Tribute to the Phospholipid

Menger, Fredric M.; Chlebowski, Mary E.; Galloway, Ashley L.; Lu, Hao; Seredyuk, Victor A.; Sorrells, Jennifer L.; Zhang, Hailing

doi:10.1021/la0508691

Cited by 32 publications

(29 citation statements)

References 31 publications

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“…Large unilamellar vesicles (LUV), typically with 100 nm diameter, prepared by the extrusion protocol described elsewhere [41], [42], were used as biomembrane models. Briefly, lipid mixtures were prepared in rounded glass-flasks and dried in vacuum overnight.…”

Section: Methodsmentioning

confidence: 99%

Intracellular Nucleic Acid Delivery by the Supercharged Dengue Virus Capsid Protein

et al. 2013

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Supercharged proteins are a recently identified class of proteins that have the ability to efficiently deliver functional macromolecules into mammalian cells. They were first developed as bioengineering products, but were later found in the human proteome. In this work, we show that this class of proteins with unusually high net positive charge is frequently found among viral structural proteins, more specifically among capsid proteins. In particular, the capsid proteins of viruses from the Flaviviridae family have all a very high net charge to molecular weight ratio (> +1.07/kDa), thus qualifying as supercharged proteins. This ubiquity raises the hypothesis that supercharged viral capsid proteins may have biological roles that arise from an intrinsic ability to penetrate cells. Dengue virus capsid protein was selected for a detailed experimental analysis. We showed that this protein is able to deliver functional nucleic acids into mammalian cells. The same result was obtained with two isolated domains of this protein, one of them being able to translocate lipid bilayers independently of endocytic routes. Nucleic acids such as siRNA and plasmids were delivered fully functional into cells. The results raise the possibility that the ability to penetrate cells is part of the native biological functions of some viral capsid proteins.

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Section: Methodsmentioning

confidence: 99%

Intracellular Nucleic Acid Delivery by the Supercharged Dengue Virus Capsid Protein

et al. 2013

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“…At this temperature and at high molar ratios (50 %), interaction is complete, since no liposomes of the initial size are detected. This can be attributed to the A C H T U N G T R E N N U N G increased lateral diffusion of dihexadecyl phosphate lipids towards the site of action [43] -that is, the dendrimeric surfacethus increasing the number of interacting guanidinium and phosphate groups. However, it is interesting to note that, contrary to expectations, at low molar ratios (5 %) no size increase is registered for the acetylated derivatives.…”

Section: Functionalization Of Poly(propylene Imine) Dendrimersmentioning

confidence: 99%

Interactive Transport of Guanidinylated Poly(propylene imine)‐Based Dendrimers through Liposomal and Cellular Membranes

et al. 2007

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The ability of guanidinylated poly(propylene imine) dendrimers to translocate across lipid bilayers was assessed by employing either a model phosphate-bearing liposomal membrane system or A549 human lung carcinoma cells. Two dendrimer generations, differing in the number of surface guanidinium groups, were employed, while surface acetylation or the use of spacers affected the binding of the guanidinium group to the phosphate moiety and finally the transport efficiency. Following adhesion of dendrimers with liposomes, fusion or transport occurred. Transport through the liposomal bilayer was observed at low guanidinium/phosphate molar ratios, and was enhanced when the bilayer was in the liquid-crystalline phase. For effective transport through the liposomal membrane, an optimum balance between the binding strength and the degree of hydrophobicity of the guanidinylated dendrimer is required. In experiments performed in vitro with cells, efficient penetration and internalization in subcellular organelles and cytosol was observed.

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“…First and foremost, the measured thermograms infer that bilayers of the DPPC liposomes are not destroyed by the formation of the supramolecular complex particles with the conjugates. What is more, the thermodynamic stability of the DPPC bilayers seems to become higher (Bai et al, 2010;Menger et al, 2005;de Oliveira Tiera, Winnik, & Tiera, 2010) in the cases of the complex particles studied. It was generally manifested itself both as the larger height (h peak ) and the larger area under the endothermic peak of the transition on the thermograms that meant the greater value of the molar enthalpy of the transition, △H tr (Fig.…”

Section: Comparison Of the Structural And Thermodynamic Parameters Ofmentioning

confidence: 88%

“…By this means DPPC bilayer shows generally the phase transition in the vicinity of 40 C (Fig. 2 and Table 6), whereas the polyunsaturated PC bilayer does not show any phase transition in the whole temperature range (from 5 C to 120 C) accessible for the measurements using the DASM-4M differential scanning calorimeter, because it has already been in the fluid liquid-crystalline state at any temperature exceeded 0 C (Menger et al, 2005). It is worthy of note here that under the temperature used for the complex formation between PC/DPPC liposomes and the particles of the conjugates, namely 40 C, the model DPPC bilayer apparently approaches maximally to the fluid liquid-crystalline state of the real polyunsaturated PC bilayer that is evidence in favor of the usefulness of DPPC as the model substance.…”

Section: Comparison Of the Structural And Thermodynamic Parameters Ofmentioning

confidence: 91%

Impact of the structure of polyunsaturated soy phospholipids on the structural parameters and functionality of their complexes with covalent conjugates combining sodium caseinate with maltodextrins

et al. 2016

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A Tribute to the Phospholipid

Cited by 32 publications

References 31 publications

Intracellular Nucleic Acid Delivery by the Supercharged Dengue Virus Capsid Protein

Intracellular Nucleic Acid Delivery by the Supercharged Dengue Virus Capsid Protein

Interactive Transport of Guanidinylated Poly(propylene imine)‐Based Dendrimers through Liposomal and Cellular Membranes

Impact of the structure of polyunsaturated soy phospholipids on the structural parameters and functionality of their complexes with covalent conjugates combining sodium caseinate with maltodextrins

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