Triboelectric nanogenerators (TENGs) can harvest mechanical energy through coupling triboelectric effect and electrostatic induction. Typically, TENGs consist of organic materials, however on account of the potentially wide range of applications of TENGs as the self-powered portable/wearable electronics, biomedical devices, and sensors; semiconductor metal oxide materials can be promising candidates to be incorporating in TENG structure. Here, flexible TENG based on self-organized TiO 2 nanotube arrays (TNTAs) is fabricated via anodization method. The introduced flexible large area nanotubular electrode is employed as the moving electrode in contact with Kapton film in vertical contact separation mode of TENG. The fabricated TENG can deliver output voltage of 40 V with the current density of 1 μA cm À2 . To evaluate the role of nanostructured interface, its performance has been compared to the thin film flat compact TiO 2 electrode. The results of extracted charge measurements under short circuit condition indicate that larger triboelectric charge density formed in TNTA-based electrode (about 110 nC per cycle of press and release) is in comparison to 15 nC in flat TiO 2 electrode. Due to the extensive range of applications of TiO 2 , the introduced structure can potentially be applicable in various types of self-powered systems such as photo-detectors and environmental gas and bio-sensors.