A Transplantation Assay for Mouse Cells Responsive to Antigenic Stimulation by Sheep Erythrocytes.

Kennedy, J. S.; Siminovitch, Louis; Till, J. E.; McCulloch, E. A.

doi:10.3181/00379727-120-30678

Cited by 62 publications

(42 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Irradiated recipients of thymus cells and SRBC will produce neither hemolytic loci nor PFC in their spleens (5,(17)(18)(19), When bone marrow is added, however, both hemolytic loci and an increase in the number of PFC per spleens can be detected (17)(18)(19). One of the differences between the population of cells from thymus and thoracic duct lymph, which could not have been demonstrated in the experimental system used in the previous study (1), is that PFC can be derived directly from some thoracic duct lymphocytes, but not from thymus cells.…”

Section: Discussionmentioning

confidence: 99%

“…The hemolytic focus assay used in the present experiments was a modification of the techniques described by Kennedy et al (5) and Playfair et al (6). The spleens were not frozen but serially sectioned into 250-/z segments by means of a tissue chopper 8 and sequentially transferred with forceps to an agar plate.…”

Section: Assays For Hemolysin Plaffue--forming Cells and Hemolytic Fomentioning

confidence: 99%

See 1 more Smart Citation

Cell to Cell Interaction in the Immune Response

Mitchell

Miller

1968

The Journal of Experimental Medicine

583

122

View full text Add to dashboard Cite

An interaction between thymus or thoracic duct cells and antibody-forming cell precursors has been implicated in the response of mice to sheep erythrocytes (1). The possibility was raised that the thymus contributes cells to the circulating pool of lymphocytes which recognize antigen (ARC) and which influence the differentiation of antibody-forming cell precursors (AFCP) to hemolysin-forming cells. The experimental system employing reconstituted neonatally thymectomized mice failed to detect the existence of AFCP in inoculated lymphoid cell suspensions.Previous work from this laboratory (2) had demonstrated that thoracic duct and spleen cells were capable of affecting the appearance of hemolytic foci in the spleens of heavily irradiated recipients injected with sheep erythrocytes. If such loci represent clusters of antibody-forming cells, then either the inoculum must contain cells potentially capable of producing hemolysins, or the host must provide AFCP which are extremely radio-resistant.In this paper, we present the results of experiments designed to determine whether both ARC and AFCP are present in populations of cells from thymus, thoracic duct lymph, or bone marrow. Materials and MethodsA,imals.--Mice of the highly inbred strains CBA and C57BL, and Ft hybrids from crosses between these strains were used. The origin and maintenance of these mice have been described in the previous paper (1).Cell suspensions were prepared as before (1). 0t~¢ra~i~e Procedures.--Thymeetomy or sham operation was performed in young adult mice, 4--6 wk old, as previously described (3). Checks for the presence of thymus remnants

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Assays For Hemolysin Plaffue--forming Cells and Hemolytic Fomentioning

confidence: 99%

Cell to Cell Interaction in the Immune Response

Mitchell

Miller

1968

The Journal of Experimental Medicine

583

122

View full text Add to dashboard Cite

show abstract

“…In fact, as previously described by several authors, injection of a limiting number of lymphoid cells in irradiated mice elicits, upon antigenic stimulation, the appearance in the spleen of discrete loci of specific antibody activity. These results have been obtained using both unprimed and primed cells (2)(3)(4)(5)(6)(7). A linear relationship is consistently obtained between number of foci per spleen and number of cells injected, thus indicating that the foci represent independent immunocompetent units.…”

Section: (From the Istituto DI Genetica Medica Universitg Di Torinomentioning

confidence: 54%

“…Under this respect, this procedure can be compared with the methods described by other authors (2,3,6) with the additional advantage that the product is labeled and can be isolated and analyzed.…”

Section: Discussionmentioning

confidence: 99%

Electrophoretically Homogeneous Antibody Synthesized by Spleen Foci of Irradiated Repopulated Mice

Luzzati

Tosi

Carbonara

1970

The Journal of Experimental Medicine

View full text Add to dashboard Cite

106 splenocytes from primed donors were injected, together with sheep erythrocytes (SRBC), into X-irradiated syngeneic mice. 8 days later the spleens were excised and cut into small fragments, keeping track of their location in the organ. Each fragment was cultured individually for 24 hr in the presence of 14C amino acids and the culture fluids were assayed for antibody activity. The antibody-producing fragments were found to be clustered in few restricted areas (foci) surrounded by negative tissue. The anti-SRBC antibody from single foci was purified by absorption on stroma followed by acid elution. Thereafter, it was subjected to electrophoresis and immunoelectrophoresis. The radioautography of the runs showed a considerable degree of homogeneity. Distinct and sharp spikes were localized in the beta and gamma region. The pattern of each focus is unique from the point of view of the number of spikes and their mobility. Eluates obtained from many pooled fragments gave a broad radioactive smear in beta-gamma region. Many foci synthesized antibody migrating as a single band. This homogeneous protein is probably the product of a clone of cells homogeneously differentiated. However, some foci producing two and probably more antibody bands were also encountered. Two interpretations of the finding can be given. Either more than one precursor may participate in the formation of a focus or a differentiation switch may occur during the clonal expansion.

show abstract

“…Celada and Wigzell (1966a and b) examined the properties of colonies of haemolytic plaque-forming cells (PFC) which appeared in the haemopoietic nodules of spleens of immunised mice recovering from sublethal irradiation, and considered that these PFC were clonally assorted. Kennedy, Siminovitch, Till and McCulloch (1965), Kennedy, Till, Siminovitch and McCulloch (1966) and Playfair, Papermaster and Cole (1965) demonstrated localised concentrations of haemolytic antibody in the spleens of irradiated mice restored with normal spleen cells and immunised with sheep erythrocytes. These haemolytic foci "Present address: Wallaceville Animal Research Centre, Private Bag, Wellington, New Zealand.…”

Section: Introductionmentioning

confidence: 99%

The Development Oe Clones Oe Antibody‐forming Cells in the Spleens Oe Irradiated Mice

Cunningham

1969

Aust J Exp Biol Med

View full text Add to dashboard Cite

Summary-A markedly non-random distribution of antibody-plaque-forming cells was found in the spleens of lethally irradiated mice which had been injected 5-10 days previously with sheep erythrocytes and 1-2 x 10" syngeneic spleen cells. Localised concentrations (colonies) of 19S ard 7S plaque-forming cells were identified by cutting the spleens transversely into 8 pieces, making dispersed-cell suspensions from these segments, and testing the suspensions directly with the haemolytic plaque technique. The colonies had an average length of approximately 0 5 mm., with an upper size limit of about 1'5 mm. There was little correlation between the position of these colonies and the position of local concentrations of haemolytic antibody detected by a haemolytic focus method.

show abstract

A Transplantation Assay for Mouse Cells Responsive to Antigenic Stimulation by Sheep Erythrocytes.

Cited by 62 publications

References 4 publications

Cell to Cell Interaction in the Immune Response

Cell to Cell Interaction in the Immune Response

Electrophoretically Homogeneous Antibody Synthesized by Spleen Foci of Irradiated Repopulated Mice

The Development Oe Clones Oe Antibody‐forming Cells in the Spleens Oe Irradiated Mice

Contact Info

Product

Resources

About