A translocation t(6;14) in two cases of leiomyosarcoma: Molecular cytogenetic and array-based comparative genomic hybridization characterization

Graaff, Marieke A. de; Jong, Daniëlle de; Bruijn, Inge H. Briaire-de; Hogendoorn, Pancras C.W.; Bovée, Judith V.M.G.; Szuhai, Károly

doi:10.1016/j.cancergen.2015.07.005

Cited by 5 publications

(1 citation statement)

References 33 publications

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“…Chromosome spreads of metaphase cells were prepared essentially as described previously (de Graaff et al, 2015) and used for karyotyping by combined binary ratio labeling of nucleic-acid probes for multi-color fluorescence in situ hybridization (COBRA-FISH) staining. The analysis used whole chromosome paint probe sets to discriminate individual chromosomes and specific staining to identify short and long arms of chromosomes, as described previously (Szuhai and Tanke, 2006).…”

Section: Huh7 Cell Karyotypingmentioning

confidence: 99%

Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture

et al. 2018

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Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA.

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Section: Huh7 Cell Karyotypingmentioning

confidence: 99%