A transgenic animal with antiviral properties that might inhibit multiple stages of infection

Jiang, Liang; Zhao, Ping; Cheng, Tingcai; Sun, Qiang; Peng, Zhengwen; Dang, Yinghui; Wu, Xiangwei; Wang, Genhong; Jin, Shengkai; Lin, Ping; Xia, Qingyou

doi:10.1016/j.antiviral.2013.02.015

Cited by 55 publications

(24 citation statements)

References 14 publications

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“…However, some pathogenic agents such as viruses, fungi, and bacteria pose serious biological challenges for this sericulture causing almost 20% losses of the potential cocoon production each year. Bombyx mori nucleopolyhedrovirus (BmNPV), a member of baculoviridae family, is the pivotal viral pathogen of B. mori that could elicit almost 80% economic loses in sericultural industry [7]. On the other hand, BmNPV has been used as an effective, feasible, and safe technology for the production of recombinant proteins in insect or insect cultured cells called BEVS (baculovirus expression vector system) [8].…”

Section: Introductionmentioning

confidence: 99%

Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus

Shobahah

Xue

et al. 2017

Virol J

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Background Bombyx mori has become an important model organism for many fundamental studies. Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen to Bombyx mori, yet also an efficient vector for recombinant protein production. A previous study indicated that acetylation plays many vital roles in several cellular processes of Bombyx mori while global phosphorylation pattern upon BmNPV infection remains elusive.MethodEmploying tandem mass tag (TMT) labeling and phosphorylation affinity enrichment followed by high-resolution LC-MS/MS analysis and intensive bioinformatics analysis, the quantitative phosphoproteome in Bombyx mori cells infected by BmNPV at 24 hpi with an MOI of 10 was extensively examined.ResultsTotally, 6480 phosphorylation sites in 2112 protein groups were identified, among which 4764 sites in 1717 proteins were quantified. Among the quantified proteins, 81 up-regulated and 25 down-regulated sites were identified with significant criteria (the quantitative ratio above 1.3 was considered as up-regulation and below 0.77 was considered as down-regulation) and with significant p-value (p < 0.05). Some proteins of BmNPV were also hyperphosphorylated during infection, such as P6.9, 39 K, LEF-6, Ac58-like protein, Ac82-like protein and BRO-D.ConclusionThe phosphorylated proteins were primary involved in several specific functions, out of which, we focused on the binding activity, protein synthesis, viral replication and apoptosis through kinase activity.

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Section: Introductionmentioning

confidence: 99%

Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus

Shobahah

Xue

et al. 2017

Virol J

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“…DNA replication and gene expression in this virus follow a cascade reaction pattern with successful infection depending on stage specific expression of BmNPV genes [2]. BmNPV infection causes high larval mortality and significantly damages to silk production [3,4]. …”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Jiang

Guo

et al. 2016

PLoS ONE

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Bombyx mori nucleopolyhedrosis virus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Since microRNAs (miRNAs) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of BmNPV infection on silkworm microRNAs expression profile. To achieve this, we constructed and deep-sequenced two small RNA libraries generated from BmNPV infected and un-infected larvae. The results revealed that 38 silkworm miRNAs were differentially expressed after BmNPV infection. Based on the GO analysis, their predicted target genes were found to be involved in diverse functions such as binding, catalytic, virion and immune response to stimulus suggesting their potential roles in host-virus interactions. Using the dual-luciferase reporter assay, we confirmed that Bmo-miR-277-5p, up-regulated in BmNPV-infected larvae, targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) gene which may play potential role in silkworm-BmNPV interaction. These results provide new insights into exploring the interaction mechanism between silkworm and BmNPV.

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“…These viruses enter the lumen and trigger the silkworm innate immunity. Many antiviral proteins such as red fluorescent protein , Bmlipase‐1 , and BmSP‐2 are induced and are important for defense again infection. In the infection process of BmNPV, occlusion‐derived virus envelope proteins (P74) fuse into the midgut epithelium.…”

Section: Introductionmentioning

confidence: 99%

Proteomics analysis of digestive juice from silkworm during Bombyx mori nucleopolyhedrovirus infection

Zhu

Wang

et al. 2015

Proteomics

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Previous studies have analyzed the midgut transcriptome and proteome after challenge with Bombyx mori nucleopolyhedrovirus (BmNPV), however little information is available on the digestive juice proteome after BmNPV challenge. This study investigated BmNPV infection-induced protein changes in the digestive juice of silkworms using shotgun proteomics and MS sequencing. From the digestive juice of normal third-day, fifth-instar silkworm larvae, 75 proteins were identified, 44 of which were unknown; from larvae 6 h after inoculation with BmNPV, 106 proteins were identified, of which 39 were unknown. After BmNPV challenge, more secreted proteins appeared that had antiviral and digestive features. GO annotation analysis clustered most proteins in the lumen into catalytic, binding, and metabolic processes. Numerous proteins were reported to have BmNPV interactions. Hsp70 protein cognate, lipase-1, and chlorophyllide A-binding protein precursor were upregulated significantly after BmNPV challenge. Levels of trypsin-like serine protease, beta-1,3-glucanase, catalase, and serine protease transcripts decreased or were not significantly change after BmNPV challenge. Taken together, these findings provided insights into the interaction between host and BmNPV and revealed potential functions of digestive juice after per os BmNPV infection.

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A transgenic animal with antiviral properties that might inhibit multiple stages of infection

Cited by 55 publications

References 14 publications

Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus

Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Proteomics analysis of digestive juice from silkworm during Bombyx mori nucleopolyhedrovirus infection

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