A transcriptomic view of the ability of nascent hexaploid wheat to tolerate aneuploidy

Zeng, Deying; Guan, Jiantao; Luo, Jiangtao; Zhao, Laibin; Li, Yazhou; Chen, Wenshuai; Zhang, Lianquan; Ning, Shunzong; Yuan, Zhongwei; Li, Aili; Zheng, Youliang; Mao, Long; Liu, Dengcai; Hao, Ming

doi:10.1186/s12870-020-2309-6

Cited by 12 publications

(7 citation statements)

References 52 publications

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“…Aneuploidy and heterozygous translocation were observed only in population QH-3. In contrast to most animal species, polyploid plant species are quite tolerant of aneuploidy (Zeng et al, 2020).…”

Section: Discussionmentioning

confidence: 95%

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Evaluation of chromosome diversity of Elymus nutans on the Qinghai‐Tibet plateau by FISH

Liu,

Jiang,

Liu

et al. 2023

Grass and Forage Science

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Elymus nutans Griseb. is an important herbage for forage and ecological restoration in the Qinghai‐Tibet Plateau (QTP). It is widely distributed in different habitats of the QTP and has been reported to have high genetic diversity in protein, morphology and DNA sequence. The main objective of our investigation has been to obtain some knowledge of E. nutans chromosome genetic diversity. In this study, the TE probe S5 and tandem repeat probe AAG combined with sequential GISH were able to successfully distinguish different subgenomes and chromosomes in 7 E. nutans accessions from major global distribution sites. On this basis, a total of 123 individuals from four representative populations of E. nutans in the QTP were hybridised. The results of the experiment showed that four types of reciprocal translocations between subgenomes were observed in the four wild populations, and the translocation chromosomes, frequency, and heterozygosis were different in each population. The distribution and quantity of S5 and AAG signals also varied within and among the four populations. The results indicated that E. nutans had high genetic diversity at the chromosome level both within and among populations, and it was speculated that non‐homologous recombination was one of the reasons for the high genetic diversity. These results provide important theoretical guidance for the collection, protection, and evaluation of E. nutans.

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Section: Discussionmentioning

confidence: 95%

“…Aneuploidy and heterozygous translocation were observed only in population QH‐3. In contrast to most animal species, polyploid plant species are quite tolerant of aneuploidy (Zeng et al, 2020). For instance, aneuploidy was found to vary from 20 to 100% among 16 new synthetic hexaploid wheat lines (Zhang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of chromosome diversity of Elymus nutans on the Qinghai‐Tibet plateau by FISH

Liu,

Jiang,

Liu

et al. 2023

Grass and Forage Science

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“…aestivum hexaploids were found to be a poor predictor of transcriptomic bias. This implies other genetic and/or epigenetic elements govern subgenome bias in wheat (Zeng et al, 2020). In allotetraploid G. hirsutum seeds, subgenome transcription bias favouring the larger A subgenome ( Gossypium arboreum ) is observed across seed development (Hovav et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals transcription factor networks and subgenome bias during Brassica napus seed development

et al. 2021

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SUMMARY We profiled the global gene expression landscape across the reproductive lifecycle of Brassica napus. Comparative analysis of this nascent amphidiploid revealed the contribution of each subgenome to plant reproduction. Whole‐genome transcription factor networks identified BZIP11 as a transcriptional regulator of early B. napus seed development. Knockdown of BZIP11 using RNA interference resulted in a similar reduction in gene activity of predicted gene targets, and a reproductive‐lethal phenotype. Global mRNA profiling revealed lower accumulation of Cn subgenome transcripts relative to the An subgenome. Subgenome‐specific transcription factor networks identified distinct transcription factor families enriched in each of the An and Cn subgenomes early in seed development. Analysis of laser‐microdissected seed subregions further reveal subgenome expression dynamics in the embryo, endosperm and seed coat of early stage seeds. Transcription factors predicted to be regulators encoded by the An subgenome are expressed primarily in the seed coat, whereas regulators encoded by the Cn subgenome were expressed primarily in the embryo. Data suggest subgenome bias are characteristic features of the B. napus seed throughout development, and that such bias might not be universal across the embryo, endosperm and seed coat of the developing seed. Transcriptional networks spanning both the An and Cn genomes of the whole B. napus seed can identify valuable targets for seed development research and that ‐omics level approaches to studying gene regulation in B. napus can benefit from both broad and high‐resolution analyses.

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“…The cytological stability appears to break down when the increase or decrease in number of chromosomes exceeds one, which may directly result in severe aneuploid states, such as nullisomy, monosomy, trisomy, and tetrasomy. Aneuploids generally arise and are rarely recovered among the progeny of newly synthesized hexaploid wheat (Zhang et al 2013 ; Zeng et al 2020 ). In previous studies, aneuploid wheat was widely used for gene localization, molecular marker development, and genetic analysis (Endo and Gill, 1996 ; Klindworth et al 2007 ; Zhang et al 2020 ; Pshenichnikova et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Chromosome karyotype and stability of new synthetic hexaploid wheat

Wang

Jia

et al. 2021

Mol Breeding

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Synthetic hexaploid wheat offers breeders ready access to potentially novel genetic variation in wild ancestral species. In this study, we crossed MY3478 (2 n = 4 x = 28, AABB) as the maternal parent with the stripe rust–resistant SY41 (2 n = 2 x = 14, DD) as the paternal parent to construct the new hexaploid wheat line NA0928 through natural allopolyploidization. Agronomic traits and the cytology of the S 8 –S 9 generations of NA0928 were analyzed. Abundant variation in agronomic traits was observed among each strain of NA0928 in the S 8 generation. Agronomic traits were superior in strains resistant to stripe rust compared with those of highly susceptible strains. The rank order of the coefficients of variation were tiller number (55.3%) > spike length (15.3%) > number of spikelets (13.9%) > plant height (8.7). Number of tillers and spike length are important traits in wheat breeding to improve yield. Cytological observation and fluorescence in situ hybridization showed that the chromosome number and configuration showed rich variation among NA0928 strains in the S 9 generation. Chromosome number ranged from 36 to 44. Variation in chromosome karyotype was detected in the A and B subgenomes. Meiotic chromosome behavior in pollen mother cells and multicolor genomic in situ hybridization revealed that two new synthetic hexaploid wheat strains showed genetic stability; one strain was resistant to stripe rust and developed multiple tillers, and the other strain was susceptible to stripe rust, but both showed improved thousand-kernel weight (TKW) weight and produced multiple tillers. The two strains will be valuable germplasm resources for use in wheat breeding.

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A transcriptomic view of the ability of nascent hexaploid wheat to tolerate aneuploidy

Cited by 12 publications

References 52 publications

Evaluation of chromosome diversity of Elymus nutans on the Qinghai‐Tibet plateau by FISH

Evaluation of chromosome diversity of Elymus nutans on the Qinghai‐Tibet plateau by FISH

Gene expression profiling reveals transcription factor networks and subgenome bias during Brassica napus seed development

Chromosome karyotype and stability of new synthetic hexaploid wheat

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