A transcriptomic taxonomy of Drosophila circadian neurons around the clock

Ma, Dingbang; Przybylski, Dariusz; Abruzzi, Katharine C.; Schlichting, Matthias; Li, Qunlong; Long, Xi; Rosbash, Michael

doi:10.7554/elife.63056

Cited by 97 publications

(265 citation statements)

References 56 publications

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“…3 E ), coupled with its fast evolution (i.e., the high d N , fig. 3 C ) and expression in the clock-neurons ( Ma et al 2021 ) may be associated with divergence in the circadian clock in species with different ecology and latitudinal distribution ( Menegazzi et al 2017 ). This might explain the findings in the agricultural pest D. suzukii , a species characterized by significant selective pressure affecting Rh6 and Rh7 ( fig.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…The absorbance of Rh7 is particularly broad with a maximum in the UV light, but with a long tail encompassing the blue and cyan wavelengths ( Sakai et al 2017 ). Rh7 is expressed in a limited number of neurons in the central brain, including some of those responsible for circadian activity ( Ni et al 2017 ; Ma et al 2021 ). Rh2 is expressed in the ocelli ( Pollock and Benzer 1988 ) and possibly in the R7 photoreceptor cells.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomics of Opsin Genes in Diptera Reveals Lineage-Specific Events and Contrasting Evolutionary Dynamics in Anopheles and Drosophila

Feuda

Goulty

Zadra

et al. 2021

Genome Biology and Evolution

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Diptera is one of the biggest insect orders and displays a large diversity of visual adaptations. Similarly to other animals, the dipteran visual process is mediated by opsin genes. While the diversity and function of these genes is well studied in key model species, a comprehensive comparative genomic study across the dipteran phylogeny is missing. Here we mined the genomes of 61 dipteran species, reconstructed the evolutionary affinities of 528 opsin genes and determined the selective pressure acting in different species. We found that opsins underwent several lineage-specific events, including an independent expansion of Long Wave Sensitive opsins in flies and mosquitoes, and numerous family-specific duplications and losses. Both the Drosophila and the Anopheles complement is derived in comparison with the ancestral dipteran state. Molecular evolutionary studies suggest that gene turnover rate, overall mutation rate, and site-specific selective pressure are higher in Anopheles than in Drosophila. Overall, our findings indicate an extremely variable pattern of opsin evolution in dipterans, showcasing how two similarly aged radiations, Anopheles and Drosophila, are characterized by contrasting dynamics in the evolution of this gene family. These results provide a foundation for future studies on the dipteran visual system.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenomics of Opsin Genes in Diptera Reveals Lineage-Specific Events and Contrasting Evolutionary Dynamics in Anopheles and Drosophila

Feuda

Goulty

Zadra

et al. 2021

Genome Biology and Evolution

View full text Add to dashboard Cite

show abstract

“…We note that such a conclusion, based on negative results, would not have been possible with existing DN1p GAL4 lines. Recent studies have demonstrated that DN1p neurons are a heterogeneous group that can be further divided into multiple subpopulations that likely make distinct contributions to behavior ( Chatterjee et al., 2018 ; Guo et al., 2018 ; Lamaze et al., 2018 ; Ma et al., 2021 ). These findings indicate that a thorough understanding of the role of DN1p neurons in circadian control will require a fine-grained analysis of different DN1p subpopulations.…”

Section: Discussionmentioning

confidence: 99%

Dorsal clock neurons in Drosophila sculpt locomotor outputs but are dispensable for circadian activity rhythms

Nettnin¹,

Sallese²,

Nasseri³

et al. 2021

iScience

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Summary The circadian system is comprised three components: a network of core clock cells in the brain that keeps time, input pathways that entrain clock cells to the environment, and output pathways that use this information to ensure appropriate timing of physiological and behavioral processes throughout the day. Core clock cells can be divided into molecularly distinct populations that likely make unique functional contributions. Here we clarify the role of the dorsal neuron 1 (DN1) population of clock neurons in the transmission of circadian information by the Drosophila core clock network. Using an intersectional genetic approach that allowed us to selectively and comprehensively target DN1 cells, we show that suppressing DN1 neuronal activity alters the magnitude of daily activity and sleep without affecting overt rhythmicity. This suggests that DN1 cells are dispensable for both the generation of circadian information and the propagation of this information across output circuits.

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“…Thus, our dpr/DIP expression could serve as a map to identify individual MNs from a MN cluster in a larval VNC sample (Nguyen et al, 2021;Vicidomini et al, 2021). In addition to dprs and DIPs, other CSP subfamilies have been reported in several scRNAseq datasets, suggesting that expression maps of other subfamilies and even combinations of subfamilies can be utilized to refine cell types in datasets (Kurmangaliyev et al, 2020;Ma et al, 2021;Xie et al, 2021).…”

Section: Using the Dpr/dip Code To Annotate Single Cell Rna Sequencing Datamentioning

confidence: 95%

Systematic expression profiling of dprs and DIPs reveals cell surface codes in Drosophila larval peripheral neurons

Wang

Lobb-Rabe

Ashley

et al. 2021

Preprint

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In complex nervous systems, neurons must identify their correct partners to form synaptic connections. The prevailing model to ensure correct recognition posits that cell surface proteins (CSPs) in individual neurons act as identification tags. Thus, knowing what cells express which CSPs would provide insights into neural development, synaptic connectivity, and nervous system evolution. Here, we investigated expression of dprs and DIPs, two CSP subfamilies belonging to the immunoglobulin superfamily (IgSF), in Drosophila larval motor neurons (MNs), sensory neurons (SNs), peripheral glia and muscles using a collection of GAL4 driver lines. We found that dprs are more broadly expressed than DIPs in MNs and SNs, and each examined neuron expresses a unique combination of dprs and DIPs. Interestingly, many dprs and DIPs are not robustly expressed, but instead, are found in gradient and temporal expression patterns. Hierarchical clustering showed a similar expression pattern of dprs and DIPs in neurons from the same type and with shared synaptic partners, suggesting these CSPs may facilitate synaptic wiring. In addition, the unique expression patterns of dprs and DIPs revealed three uncharacterized MNs - MN23-Ib, MN6-Ib (A2) and MN7-Ib (A2). This study sets the stage for exploring the functions of dprs and DIPs in Drosophila MNs and SNs and provides genetic access to subsets of neurons.

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A transcriptomic taxonomy of Drosophila circadian neurons around the clock

Cited by 97 publications

References 56 publications

Phylogenomics of Opsin Genes in Diptera Reveals Lineage-Specific Events and Contrasting Evolutionary Dynamics in Anopheles and Drosophila

Phylogenomics of Opsin Genes in Diptera Reveals Lineage-Specific Events and Contrasting Evolutionary Dynamics in Anopheles and Drosophila

Dorsal clock neurons in Drosophila sculpt locomotor outputs but are dispensable for circadian activity rhythms

Systematic expression profiling of dprs and DIPs reveals cell surface codes in Drosophila larval peripheral neurons

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