A transcriptomic analysis of malignant transformation of human embryonic esophageal epithelial cells by HPV18 E6E7

Abstract

Background: Esophageal cancer is one of the most common malignant tumours in humans. A series of esophageal cancer cell lines are accompanied by human papilloma virus (HPV) infection, but the mechanism behind HPV in cancer malignancy is not clear.Methods: This research was conducted in different generations of HPV E6E7 gene-induced human foetal esophageal epithelial immortalised cells (Shantou Human Embryonic Esophageal Epithelial cell line; SHEE); the RNA sequencing transcriptomic analysis was performed to explore the mechanism of HPV infection in these cell lines. Results:The results showed that there are 9,990 differential genes in late-stage cells compared with HPV18 E6E7-infected early foetal esophageal epithelial immortalised cells. Among these, 4,882 genes are upregulated, and 5,108 genes are downregulated. We used bioinformatics to analyze the expression and function of aberrantly expressed lncRNA, miRNA, mRNA and construct the competing endogenous RNA (ceRNA) network and protein protein interaction (PPI) network.Conclusions: we predicted TP53TG1 promotes to malignant transformation of SHEEs by acting as a ceRNA to competitively bind to miR-6835 and regulate IGF2 expression. We also predicted IL6 serve as prognostic biomarkers and therapy target. With these results maybe provides new insights into the mechanisms of HPV carcinogenesis in esophageal cancer.

Aberrant plasma exosomal derived ceRNA networks as diagnosis biomarkers for adolescent major depressive disorder and its potential prediction for antidepressant

“…The previous studies showed that CCDC18-AS1 was associated with immune microenvironment, and was positively correlated with activated memory CD4 T cell, activated myeloid dendritic cell, neutrophils, macrophage M1, and T follicular helper cells, while negatively correlated with T regulatory cell(68) (69). The miR-6835-5p could bind to Insulin-like growth factor 2 (IGF2) (70). IGF2 belongs to the insulin growth factor family, which can modulate the proliferation, survival, and differentiation of many types of neuronal systems, including cholinergic, dopaminergic, and serotonergic neurons (71).…”

Identification of Aberrant Plasma Exosomal Derived ceRNA Networks in Adolescent with Major Depressive Disorder

“…It exhibits a positive correlation with activated memory CD4 T cells, activated myeloid dendritic cells, neutrophils, M1 macrophages, and follicular helper T cells, while displaying a negative correlation with regulatory T cells (46) (47). miR-6835-5p has the capacity to interact with insulin-like growth factor 2 (IGF2) (48). Insulin-like growth factor 2 (IGF2) is a constituent of the insulin-like growth factor family and governs the proliferation, survival, and differentiation of diverse neuronal systems, encompassing cholinergic, dopaminergic, and serotonergic neurons (49).…”

LncRNA TP53TG1 plays an anti-oncogenic role in cervical cancer by synthetically regulating transcriptome profile in HeLa cells

“…However, TP53TG1 may act as a tumor suppressor gene or an oncogene in different tumors ( Zhang et al, 2019b ; Lu et al, 2021 ). It functions as an oncogene in pancreatic ductal adenocarcinoma (PDAC), retinoblastoma, and nasopharyngeal carcinoma ( Yuan et al, 2017 ; Zhang et al, 2019b ; Shao et al, 2020 ; Tang et al, 2020 ; Cheng et al, 2021a ; Gao et al, 2021 ; Lu et al, 2021 ; Wang et al, 2021 ). In some other cancers, such as non-small cell lung cancer, hepatocellular carcinoma, gastrointestinal cancer, and cutaneous melanoma, TP53TG1 serves as a cancer suppressor ( Benfodda et al, 2018 ; Xiao et al, 2018 ; Chen et al, 2021 ; Masoumi et al, 2021 ).…”