A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae.

Smith, Harold E.; Mitchell, A P

doi:10.1128/mcb.9.5.2142

Cited by 165 publications

(222 citation statements)

References 56 publications

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“…The negative regulation of KIN28±CCL1 also contributes to proper development of meiosis. IME1 is regulated negatively by CLN3 and IME2 at transcriptional and nuclear import levels (Colomina et al, 1999) and at transcriptional level (Smith and Mitchell, 1989), respectively promoter-driven overexpression of KIN28±CCL1 indirectly cancelled the negative regulation of IME2. Alternatively, induction of IME2 is positively regulated by external alkalization through SRB10±SRB11 independently of KIN28±CCL1 and IME1.…”

Section: Discussionmentioning

confidence: 99%

“…It also induces meiosis-speci®c genes called IME1 and IME2 (Hayashi et al, 1998b), which are speci®cally expressed early in the meiotic development and required for the entry into meiosis. IME1 activates transcription of IME2 (Smith and Mitchell, 1989;Yoshida et al, 1990), encoding the meiosis-speci®c protein kinase (Kominami et al, 1993), and both are required for premeiotic DNA synthesis and subsequent meiotic development (Mitchell, 1994). These results therefore indicate that yeast cells communicate with each other by producing carbon dioxide and elevating medium pH in order to undergo G 1 arrest and meiosis.…”

Section: Introductionmentioning

confidence: 99%

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A transcriptional autoregulatory loop for KIN28–CCL1 and SRB10–SRB11, each encoding RNA polymerase II CTD kinase–cyclin pair, stimulates the meiotic development of S. cerevisiae

Ohkuni¹,

Yamashita²

2000

Yeast

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Alkalization of the medium is associated with and required for the cellular development to meiosis and sporulation in the yeast Saccharomyces cerevisiae. To elucidate the molecular mechanisms for the signi®cance of external alkalization, we isolated mutants defective in division arrest at G 1 phase under an alkaline condition. The mutants obtained had recessive alleles of SRB10 encoding the cyclin (SRB11)-dependent protein kinase that phosphorylates the CTD domain of the largest subunit of RNA polymerase II and negatively regulates the transcriptional initiation of certain genes. A Dsrb11 deletion mutant showed the same cell cycle defect. When shifted to alkali, wild-type cells decreased transcript levels of G 1 -cyclin genes (CLN1 to CLN3) and KIN28±CCL1 (encoding another CTD kinase±cyclin pair which, in contrast, stimulates the promoter clearance and transcriptional elongation in most genes), resulting in the accumulation of G 1 cells and the hypophosphorylated form of RNA polymerase II and in an increase in cell size. However, under the same conditions, a Dsrb10 mutant was defective in these events, except the downregulation of CLN1 and CLN2. The Dsrb10 mutation also in¯uenced on the transcript levels of meiosis-inducing genes called IME1 and IME2: the mutation elevated the transcript level of IME1 but reduced that of IME2, resulting in partial defects in premeiotic DNA synthesis and meiosis. Overexpression of KIN28 and CCL1 in wild-type cells impaired the alkali-induced G 1 arrest and the rate of meiosis and elevated the transcript levels of SRB11 and IME1. These results indicate that a transcriptional autoregulatory loop for KIN28±CCL1 and SRB10±SRB11 is important for G 1 arrest and meiosis. We also found that environmental conditions for meiosis ®nely regulate the transcript levels of KIN28 and CCL1, such that nitrogen starvation ®rst elevates them but subsequent alkalization of medium decreases them.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A transcriptional autoregulatory loop for KIN28–CCL1 and SRB10–SRB11, each encoding RNA polymerase II CTD kinase–cyclin pair, stimulates the meiotic development of S. cerevisiae

Ohkuni¹,

Yamashita²

2000

Yeast

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“…Our data do not address whether nitrogen repressed IME2 transcription independently of IME1, since IME2 is a downstream gene the transcription of which requires 1M E1 function. 4 ,5) However, the complete inhibition by glucose of IME2 transcription (Fig. 3b) might occur, to some extent, independently of the IME1 regulation.…”

Section: Discussionmentioning

confidence: 99%

“…5) We report here studies on the remaining two plasmids (pSP2 and pSP3). Restriction mapping analysis (described below) of the yeast inserts cloned on pSP2 and pSP3 found no similarity to IMEl,3) IME2, 4,5) or MCKl,14) and therefore represents new loci The a/a diploid cells (AMI205-9B x YIY344) were transformed with each plasmid (e, pSPI; ... , pSP2; ., pSP3; and 0, pYIl). The plasmids pSPl, pSP2, and pSP3…”

Section: Cloning Of Genes Which When Present In Increased Dosage Bymentioning

confidence: 99%

Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae

Kawaguchi

Yoshida

Yamashita

1992

Bioscience, Biotechnology, and Biochemistry

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“…Northern Blots-For Northern analysis, 15-20 gs of total RNA per lane was run on dentaturing formaldehyde gels, transferred to a nylon membrane and hybridized as described (37). To generate Northern blot probes, genomic DNA was PCR-amplified using the primers designated with "N" followed by the gene name in Table II.…”

Section: Methodsmentioning

confidence: 99%

Alkaline Response Genes of Saccharomyces cerevisiaeand Their Relationship to the RIM101 Pathway

Lamb¹,

Xu²,

Diamond³

et al. 2001

Journal of Biological Chemistry

220

243

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Environmental pH exerts broad control over growth and differentiation, but the molecular responses to external pH changes are poorly understood. Here we have used open reading frame macroarray hybridization to identify alkaline response genes in Saccharomyces cerevisiae. Northern or lacZ fusion assays confirmed the alkaline induction of two ion pump genes (ENA1 and VMA4), several ion limitation genes (CTR3, FRE1, PHO11/12, and PHO84), a siderophore-iron transporter gene (ARN4/ENB1), two transcription factor genes (NRG2 and TIS11), and two predicted membrane protein genes (YAR068W/YHR214W and YOL154W). Unlike ENA1 and SHC1, these new alkaline response genes are not induced by high salinity. The known pH-responsive genes in other fungi depend on the conserved PacC/ Rim101p transcription factor, but induction of several of these new genes relied upon Rim101p-independent pH signaling mechanisms. Rim101p-dependent genes were also dependent on Rim13p, a protease required for Rim101p processing. The Rim101p-dependent gene VMA4 is required for growth in alkaline conditions, illustrating how Rim101p may control adaptation. Because Rim101p activates ion pump genes, we tested the role of RIM101 in ion homeostasis and found that RIM101 promotes resistance to elevated cation concentrations. Thus, gene expression surveys can reveal new functions for characterized transcription factors in addition to uncovering physiological responses to environmental conditions.

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A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae.

Cited by 165 publications

References 56 publications

A transcriptional autoregulatory loop for KIN28–CCL1 and SRB10–SRB11, each encoding RNA polymerase II CTD kinase–cyclin pair, stimulates the meiotic development of S. cerevisiae

A transcriptional autoregulatory loop for KIN28–CCL1 and SRB10–SRB11, each encoding RNA polymerase II CTD kinase–cyclin pair, stimulates the meiotic development of S. cerevisiae

Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae

Alkaline Response Genes of Saccharomyces cerevisiaeand Their Relationship to the RIM101 Pathway

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