A traditional Chinese formula composed of Chuanxiong Rhizoma and Gastrodiae Rhizoma (Da Chuanxiong Formula) suppresses inflammatory response in LPS -induced RAW 264.7 cells through inhibition of NF-κB pathway

Liu, Zhi Ke; Ng, Chun Fai; Shiu, Hoi Ting; Wong, Hai Ming; Wong, Chun Wai; Li, Kai Kai; Zhang, Jin Fang; Lam, P. M.; Poon, Wai Sang; Lau, Clara Bik‐San; Leung, Ping Chung; Ko, Chun‐Hay

doi:10.1016/j.jep.2016.12.014

Cited by 47 publications

(30 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also inhibits inflammation and nerve cell apoptosis . In lipopolysaccharide (LPS)‐incited RAW 264.7 cells, DCXF inhibited the productions of NO and PGE2 by suppressing COX‐2 and iNOS expressions …”

Section: Resultsmentioning

confidence: 99%

“…15 3.9 | Da Chuanxiong Formula DCXF in Chinese traditional medicine consists of two dried rhizomes of Ligusticum chuanxiong and Gastrodia elata (G. elata) at a ratio of 4:1 (w/w). 111 Studies have shown that DCXF possesses therapeutic effects on stroke, dementia, vertigo, and headache, and is mediated by improvement of blood vessel elasticity and cerebral blood supply, reduction of bloodbrain barrier (BBB) disruption, intracellular free calcium concentration, and edema formation. It also inhibits inflammation and nerve cell apoptosis.…”

Section: Crocus Sativusmentioning

confidence: 99%

“…112 In lipopolysaccharide (LPS)-incited RAW 264.7 cells, DCXF inhibited the productions of NO and PGE2 by suppressing COX-2 and iNOS expressions. 111 Treatment with DCXF aqueous extract 1 week before and 11 days after the induction TBI by a CCI model in rat improved the learning ability, memory retention, and proliferation of neural stem cells (NSCs). Results also showed that DCXF reduced activation of astrocytes and microglia, BBB permeability, brain edema, and neuronal loss in the brain with TBI.…”

Section: Crocus Sativusmentioning

confidence: 99%

See 2 more Smart Citations

Medicinal plants in traumatic brain injury: Neuroprotective mechanisms revisited

et al. 2019

View full text Add to dashboard Cite

Traumatic brain injury (TBI) is the most prevalent health problem affecting all age groups, and leads to many secondary problems in other organs especially kidneys, gastrointestinal tract, and heart function. In this review, the search terms were TBI, fluid percussion injury, cold injury, weight drop impact acceleration injury, lateral fluid percussion, cortical impact injury, and blast injury. Studies with Actaea racemosa, Artemisia annua, Aframomum melegueta, Carthamus tinctorius, Cinnamomum zeylanicum, Crocus sativus, Cnidium monnieri, Curcuma longa, Gastrodia elata, Malva sylvestris, Da Chuanxiong Formula, Erigeron breviscapus, Panax ginseng, Salvia tomentosa, Satureja khuzistanica, Nigella sativa, Drynaria fortune, Dracaena cochinchinensis, Polygonum cuspidatum, Rosmarinus officinalis, Rheum tanguticum, Centella asiatica, and Curcuma zedoaria show a significant decrease in neuronal injury by different mechanisms such as increasing superoxide dismutase and catalase activities, suppressing nuclear factor kappa B (NF‐κB), interleukin 1 (IL‐1), glial fibrillary acidic protein, and IL‐6 expression. The aim of this study was to evaluate the neuroprotective effects of medicinal plants in central nervous system pathologies by reviewing the available literature.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Crocus Sativusmentioning

confidence: 99%

Section: Crocus Sativusmentioning

confidence: 99%

See 1 more Smart Citation

Medicinal plants in traumatic brain injury: Neuroprotective mechanisms revisited

et al. 2019

View full text Add to dashboard Cite

show abstract

“…erefore, the principle of traditional Chinese medical therapy is to boost Qi, move the blood, regulate the spirit, and then sharpen cognition. In present study, nine Chinese herbs were selected to evaluate the potential for treatment of AD because they were most frequently used to improve the kidney function in books of TCM [17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Anti‐AChE and Antioxidative Effects of Schisandra chinensis Extract: A Potential Candidate for Alzheimer’s Disease

Song

Wang

Guo

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Acetylcholinesterase (AChE) inhibition and antioxidants are two common strategies for the treatment in the early stage of Alzheimer’s Disease (AD). In this study, extracts from nine traditional Chinese medical (TCM) herbs were tested for anti-AChE activity by Ellman’s microplate assay and cytotoxicity by CCK-8. Based on its excellent AChE inhibition effect and its lowest cytotoxicity, Schisandra chinensis (SC) extract was selected to do the mechanism research. SC extract protected pheochromocytoma (PC12) cells against H2O2-induced toxicity by improving the cell survival rate in a dose-dependent manner. And it also showed significant free radical (DPPH) scavenging activities, ferric reducing antioxidant power (FRAP), and 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging. To confirm these results, the scopolamine-induced mice models were utilized in this study. Compared with the positive drug (piracetam), SC could also exhibit similar effects to alleviate the mice’s cognitive deficits. Moreover, in the mice brain samples, the AChE activity and malondialdehyde (MDA) levels of SC-treatment group both showed a reverse as compared to model group. Taken together, these results all suggested that SC extract may be a potential therapeutic candidate for AD.

show abstract

“…On the other hand, network pharmacology has become a novel branch of systems biology, particularly used in the identi cation of e cient targets and their pathways of Chinese formulas [5][6] . Twenty active compounds of a couplet medicinals, Gastrodia elata and Ligusticum chuanxiong Hort, were annoted to 48 molecular targets which are associated with migraine headaches by network pharmacology [7][8] . The couplet of Gastrodia elata and Ramulus Uncariae demonstrated multi-target and multi-pathway regulation for the therapy of cerebral ischemia [9] .…”

Section: Introductionmentioning

confidence: 99%

The Anti-RA Activities of a Couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata, Explored by Untargeted Metabolomics and Network Pharmacology.

Yang¹,

Shen

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata (GERA), is an established formula extensively used in Chinese medicine for treating rheumatoid arthritis (RA). However, the anti-RA mechanisms of GERA are still unclear. This paper aims to explore the anti-RA mechanisms of GERA by a combined strategy of untargeted metabolomics and network pharmacology. Methods Three water extracts were prepared as propotions: Gastrodia Elata compared with Radix Aconitic Lateralis (w/w): 1:1, 3:2, or 2:3. The untargeted metabolomics were executed with UPLC-MS. The metabolites were annotated and identified by Human metabolome database (HMDB) and Lipid maps database. Finally, key genes and pathways related to anti-RA activities of GERA were mined by network pharmacology. Results The untargeted metabolomics profile displayed that four differentially expressed metabolites were involved in isoflavonoid biosynthesis and biosynthesis of unsaturated fatty acids (p < 0.05). Among these differential metabolites, the essential ingredients of GERA were linoleic acid, daidzein, and daidzin. The principal targets of anti-RA activities of GERA were IL-6, TNF, VEGFA, TP53, CASP3 and PTGS2. Thirty anti-RA targets of GERA were majorly belonged to pathways response for anti-inflammation, endothelial function and apoptosis, suggesting the fundamental process of RA treatment. Conclusion The anti-RA activities of GERA were based on the inhibition of inflammation and regulation of endothelial function and apoptosis.

show abstract

A traditional Chinese formula composed of Chuanxiong Rhizoma and Gastrodiae Rhizoma (Da Chuanxiong Formula) suppresses inflammatory response in LPS -induced RAW 264.7 cells through inhibition of NF-κB pathway

Cited by 47 publications

References 47 publications

Medicinal plants in traumatic brain injury: Neuroprotective mechanisms revisited

Medicinal plants in traumatic brain injury: Neuroprotective mechanisms revisited

In Vitro and In Vivo Anti‐AChE and Antioxidative Effects of Schisandra chinensis Extract: A Potential Candidate for Alzheimer’s Disease

The Anti-RA Activities of a Couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata, Explored by Untargeted Metabolomics and Network Pharmacology.

Contact Info

Product

Resources

About