A tooth-binding antimicrobial peptide to prevent the formation of dental biofilm

Zhang, Li-Yu; Fang, Zehui; Li, Quanli; Cao, Chris Ying

doi:10.1007/s10856-019-6246-6

Cited by 39 publications

(33 citation statements)

References 35 publications

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“…Jannadi et al [89] synthesized Pep19-2.5 and Pep19-4LF and assessed that they inhibited S. mutans growth and biofilm formation. Similarly, Zhang et al [39] designed DPS-PI, Liang et al [90] designed LR-10, Da Silva et al [91] designed [W7]KR12-KAEK, and all of their antibacterial activities were evaluated by assessing the inhibition of S. mutans growth and biofilm biomass, furthermore assessing the destruction to biofilm morphology and the damage to the bacterial surface via scanning electron microscopy. In this current study, GERM CLEAN showed capabilities on reducing the initial adherence and disrupting the biofilm formation of S. mutans.…”

Section: Discussionmentioning

confidence: 99%

“…To improve these circumstances, taking natural AMPs as templates, many scholars have successfully designed and created many synthetic AMPs with promising antibacterial activity [35][36][37]. ese years, Chen et al [38] designed ZXR-2, Sullivan et al [27] synthesized C16G2, and Zhang et al [39] created DPS-PI, which were all synthetic AMPs that showed apparent antibacterial effect against the caries pathogenic bacteria, S. mutans. When compared with natural AMPs, synthetic AMPs can possess more efficient and broader-spectrum antibacterial activity and are not easy to produce resistance limitation, with lower cytotoxicity [34,35,40].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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Objective. To investigate the antibacterial effect of a novel antimicrobial peptide containing oral spray GERM CLEAN on Streptococcus mutans (S. mutans) in vitro and further explore the related mechanisms at phenotypic and transcriptional levels. Methods. The disk diffusion method was used to preliminarily appraise the antimicrobial effect of GERM CLEAN. The minimal inhibitory concentration (MIC) of GREM CLEAN towards S. mutans was determined by the broth dilution method. S. mutans virulence-related phenotypic assays including initial adhesive assay, pH drop, exopolysaccharides (EPS), and biofilm formation measurements and quantitative real-time PCR (qRT-PCR) were further applied to detect the inhibitory mechanisms of GREM CLEAN at 1/2MIC. Results. The diameter (10.18 ± 1.744 mm) of inhibition zones formed by GERM CLEAN preliminarily indicated its inhibitory effect on the major cariogenic bacteria S. mutans. The minimal inhibitory concentration of GERM CLEAN on S. mutans was 100% mass fraction (the stock solution). The study of the antibacterial mechanism showed that GERM CLEAN had a certain inhibitory effect on the initial adhesion, acid production, extracellular polysaccharides (EPS) production, and biofilm formation of S. mutans. GERM CLEAN disturbed S. mutans biofilm physiology mainly through destruction of biofilm architecture and suppression of bacterial growth. The results of qRT-PCR further confirmed that the expression levels of EPS and lactic acid generation genes including gtfB, gtfC, gtfD, and ldh were significantly repressed by treating with GERM CLEAN, and this was consistent with our phenotypic results. Conclusion. The novel antimicrobial peptide containing oral spray GERM CLEAN has an anti-Streptococcus mutans effect and the inhibitory property may be due to suppression of the virulence factors of S. mutans including adhesive, acidogenicity, EPS, and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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“…Other studies involved developing tooth‐binding antimicrobial peptides by grafting a hydroxyapatite‐binding antimicrobial peptide to a broad‐spectrum antimicrobial peptide domain. This was done to achieve the long‐term retention of antimicrobial peptides in the oral cavity (Huang et al., 2016; Zhang et al., 2019).…”

Section: Inhibition Of Cariogenic Species and Promotion Of Pulp Tissue Healingmentioning

confidence: 99%

The multifaceted roles of antimicrobial peptides in oral diseases

Niu

Yin

Mei

et al. 2021

Molecular Oral Microbiology

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Antimicrobial peptides are naturally occurring protein molecules with antibacterial, antiviral and/or antifungal activity. Some antimicrobial peptides kill microorganisms through direct binding with negatively charged microbial surfaces. This action disrupts the cytoplasmic membrane and leads to the leakage of the cytoplasm. In addition, they are involved in the innate immune response. Antimicrobial peptides play an important role in oral health, as natural antimicrobial peptides are the first line of host defence in response to microbial infection. The level of natural antimicrobial peptides increases during severe disease conditions and play a role in promoting the healing of oral tissues. However, they are insufficient for eliminating pathogenic micro-organisms. The variability of the oral environment can markedly reduce the effect of natural antimicrobial peptides. Thus, researchers are developing synthetic antimicrobial peptides with promising stability and biocompatibility. Synthetic antimicrobial peptides are a potential alternative to traditional antimicrobial therapy.Pertinent to oral diseases, the deregulation of antimicrobial peptides is involved in the pathogenesis of dental caries, periodontal disease, mucosal disease and oral cancer, where they can kill pathogenic microorganisms, promote tissue healing, serve as biomarkers and inhibit tumor cells. This narrative review provides an overview of the multifaceted roles of antimicrobial peptides in oral diseases.

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“…Others have developed a peptide combining an AMP domain and a domain with high affinity for hydroxyapatite to engineer antimicrobial enamel. [414][415][416] Other similar strategies have been developed using monomers. 417,418 Additional work developed a coating process for dentin whereby lysozyme (an antimicrobial enzyme part of the immune system) 419 is emulsified in a solution of PEG (polyethylene glycol) to form amyloid-like lysozyme oligomer aggregates and result in an antifouling coating against proteins and S. mutans and induce remineralization under specific conditions.…”

Section: Oral Hard Tissue Modification With Biomoleculesmentioning

confidence: 99%