A Toolbox forNeisseria meningitidis: Gene Editing, Complementation and Labelling
Morgane Wuckelt,
Audrey Laurent,
Clémence Mouville
et al.
Abstract:The efficient transformation of Neisseria meningitidis and Neisseria gonorrhoeae facilitates the rapid construction of bacterial mutants with insertion of antibiotic resistance cassettes. However, this strategy limits the construction of strains with multiple mutations. Recent advances in markerless strategies for Neisseria species have enabled the construction of mutants without antibiotic resistance markers. However, these innovative approaches have potential limitations related to the selection strategy or … Show more
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