A Toolbox for Constructing a Stable Genetic Transformation Platform Allowing Foreign Fragment Integration in the Genome of Neopyropia yezoensis

Cao, Xuexue; Kong, Fanna; Sun, Bin; Yin, Jiqiang; Ren, Hongzhong; Yue, Huijie; Yu, Chunhao; Tang, Xiufeng; Du, Guoying; Wang, Dongmei; Mao, Yunxiang

doi:10.3389/fmars.2022.856790

Cited by 3 publications

(2 citation statements)

References 60 publications

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“…Most of the genetic engineering efforts have focused on microalgae, macroalgae are only starting to get some attention (Charrier et al, 2015;Cao et al, 2022). Significant efforts are underway to engineer microalgae for biofuel production, despite considerable scientific challenges resulting in several unsuccessful commercialization attempts.…”

Section: Algaementioning

confidence: 99%

Perspectives on biorefineries in microbial production of fuels and chemicals

Decker,

Brunecky,

Yarbrough

et al. 2023

Front. Ind. Microbiol.

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Microbes drive our complex biosphere by regulating the global ecosystem through cycling elements and energy. Humankind has barely begun leveraging this biotransformation capacity to impact global economies and ecologies. Advances in genetic engineering, molecular analysis, metabolic flux modeling, microbial consortia/biome mapping and engineering, cell-free bioproduction, artificial intelligence/machine learning and the ever expanding -omics frontiers have set the stage for paradigm changes to how humankind produces, uses, transforms, and recycles carbon and energy through microbes. Harnessing this enormous potential could drive a global bioeconomy and manage carbon at a planetary level but requires understanding and application at a grand scale across a broad range of science and engineering disciplines. The penultimate manifestation of these advances is the “bio-refinery”, which is often referenced, but is a long way from being fully developed as a global carbon management platform. Broadening the feed stocks, processing operations, and product portfolio to a sequential cascade optimizing the conversion as a whole instead of limited outputs could greatly advance deployment and stability of a bioeconomy.

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Section: Algaementioning

confidence: 99%

Perspectives on biorefineries in microbial production of fuels and chemicals

Decker,

Brunecky,

Yarbrough

et al. 2023

Front. Ind. Microbiol.

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“…In the red seaweed P. yezoensis , overexpression of endogenous genes has proven to be feasible by Zheng et al (2022) . Another report by Cao et al (2022) , optimized a biolistic protocol to allow a high-efficiency stable transformation in this species. However, it should be noted that only reporter genes were used in this study.…”

Section: Introductionmentioning

confidence: 99%

Genome editing in macroalgae: advances and challenges

De Saeger,

Coulembier Vandelannoote,

Lee

et al. 2024

Front. Genome Ed.

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This minireview examines the current state and challenges of genome editing in macroalgae. Despite the ecological and economic significance of this group of organisms, genome editing has seen limited applications. While CRISPR functionality has been established in two brown (Ectocarpus species 7 and Saccharina japonica) and one green seaweed (Ulva prolifera), these studies are limited to proof-of-concept demonstrations. All studies also (co)-targeted ADENINE PHOSPHORIBOSYL TRANSFERASE to enrich for mutants, due to the relatively low editing efficiencies. To advance the field, there should be a focus on advancing auxiliary technologies, particularly stable transformation, so that novel editing reagents can be screened for their efficiency. More work is also needed on understanding DNA repair in these organisms, as this is tightly linked with the editing outcomes. Developing efficient genome editing tools for macroalgae will unlock the ability to characterize their genes, which is largely uncharted terrain. Moreover, given their economic importance, genome editing will also impact breeding campaigns to develop strains that have better yields, produce more commercially valuable compounds, and show improved resilience to the impacts of global change.

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Overexpression of MPV17/PMP22‐like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)

Li,

Yang,

Sun

et al. 2024

Journal of Phycology

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The northward shift of Pyropia yezoensis aquaculture required the breeding of germplasms with tolerance to the oxidative stress due to the high light conditions of the North Yellow Sea area. The MPV17/PMP22 family proteins were identified as a molecule related to reactive oxygen species (ROS) metabolism. Here, one of the MPV17 homolog genes designated as PyM‐LP2 was selected for functional identification by introducing the encoding sequence region/reverse complementary fragment into the Py. yezoensis genome. Although the photosynthetic activity, the respiratory rate, and the ROS level in wild type (WT) and different gene‐transformed algal strains showed similar levels under normal conditions, the overexpression (OE) strain exhibited higher values of photosynthesis, respiration, and reducing equivalents pool size but lower intracellular ROS production under stress conditions compared with the WT. Conversely, all the above parameters showed opposite variation trends in RNAi strain as those in the OE strain. This implied that the PyM‐LP2 protein was involved in the mitigation of the oxidative stress. Sequence analysis revealed that this PyM‐LP2 protein was assorted to peroxisomes and might serve as a poring channel for transferring malate (Mal) to peroxisomes. By overexpressing PyM‐LP2, the transfer of Mal from chloroplasts to peroxisomes was enhanced under stress conditions, which promoted photorespiration and ultimately alleviated excessive reduction of the photosynthetic electron chain. This research lays the groundwork for the breeding of algae with enhanced resistance to oxidative stresses.

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A Toolbox for Constructing a Stable Genetic Transformation Platform Allowing Foreign Fragment Integration in the Genome of Neopyropia yezoensis

Cited by 3 publications

References 60 publications

Perspectives on biorefineries in microbial production of fuels and chemicals

Perspectives on biorefineries in microbial production of fuels and chemicals

Genome editing in macroalgae: advances and challenges

Overexpression of MPV17/PMP22‐like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)

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