A Tissue-Specific Rhythmic Recruitment Pattern of Leukocyte Subsets

Yuan, Yinglin; Wu, Shengwang; Li, Weiwei; He, Wenyan

doi:10.3389/fimmu.2020.00102

Cited by 15 publications

(14 citation statements)

References 41 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in the study that confirmed an increased production of EV during HIV infection, the sampling was done essentially during the day [ 50 ]. Physiologically, the number of the major leukocyte subsets in blood, including neutrophils, monocytes, CD4 T cell, CD8 T cell, NK cells, and eosinophils varies throughout the day, reflecting bone marrow output and emigration from the blood into tissues [ 51 , 52 , 53 ]. This spatiotemporal distribution of leukocyte subsets is driven by the circadian expression of pro-migratory molecules expressed on the leukocytes and the endothelium [ 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

“…Physiologically, the number of the major leukocyte subsets in blood, including neutrophils, monocytes, CD4 T cell, CD8 T cell, NK cells, and eosinophils varies throughout the day, reflecting bone marrow output and emigration from the blood into tissues [ 51 , 52 , 53 ]. This spatiotemporal distribution of leukocyte subsets is driven by the circadian expression of pro-migratory molecules expressed on the leukocytes and the endothelium [ 52 , 53 ]. Systemic circadian signals and cell-intrinsic molecule clocks contribute to the oscillatory expression of these pro-migratory molecules [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV

et al. 2021

View full text Add to dashboard Cite

Background: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV−). Methods: Venous blood samples from n = 10 HIV+ART and n = 10 HIV− participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR. Results: Large EVs were significantly bigger in the plasma collected at 10:00 versus 22:00 in both groups. There was a significant day–night increase in the quantity of 5 miRNAs in HIV− large EVs. In HIV+ART, only miR-155 daily variation has been observed in large EVs. Finally, EV-miRNA content permits to distinguish HIV− to HIV+ART in multivariate analysis. Conclusion: These results point that plasma EV amount and microRNA contents are under daily variation in HIV− people. This new dynamic measure is disrupted in PLWH despite viral-suppressive ART. This study highlights a significant difference concerning EV abundance and their content measured at 22:00 between both groups. Therefore, the time of blood collection must be considered in the future for the EV as biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV

et al. 2021

View full text Add to dashboard Cite

show abstract

“…It is well established that leukocyte trafficking follows a circadian oscillation [reviewed extensively in (3,4,25)]. The expression of circadian clock genes is ubiquitous to nearly all immune cells, and clocks can directly regulate immune cell trafficking.…”

Section: Circadian Regulation Of Leukocyte Trafficking In Ageingmentioning

confidence: 99%

Changes in Circadian Rhythms Dysregulate Inflammation in Ageing: Focus on Leukocyte Trafficking

et al. 2021

View full text Add to dashboard Cite

Leukocyte trafficking shows strong diurnal rhythmicity and is tightly regulated by circadian rhythms. As we age, leukocyte trafficking becomes dysregulated, contributing to the increased systemic, low-grade, chronic inflammation observed in older adults. Ageing is also associated with diminished circadian outputs and a dysregulation of the circadian rhythm. Despite this, there is little evidence to show the direct impact of age-associated dampening of circadian rhythms on the dysregulation of leukocyte trafficking. Here, we review the core mammalian circadian clock machinery and discuss the changes that occur in this biological system in ageing. In particular, we focus on the changes that occur to leukocyte trafficking rhythmicity with increasing age and consider how this impacts inflammation and the development of immune-mediated inflammatory disorders (IMIDs). We aim to encourage future ageing biology research to include a circadian approach in order to fully elucidate whether age-related circadian changes occur as a by-product of healthy ageing, or if they play a significant role in the development of IMIDs.

show abstract

“…Due to the complexity and universality of the circadian rhythm system, the study of its molecular mechanism is challenging. Although increasing evidence suggests that circadian dysregulation affects the immune system [14,15], the exact mechanism of the interaction between miRNAs, clock genes, and glioma progression remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Microglial Exosome miR-7239-3p Promotes Glioma Progression by Regulating Circadian Genes

Guan

Jiang

et al. 2021

Neurosci. Bull.

View full text Add to dashboard Cite

Glioma-associated microglial cells, a key component of the tumor microenvironment, play an important role in glioma progression. In this study, the mouse glioma cell line GL261 and the mouse microglia cell line BV2 were chosen. First, circadian gene expression in glioma cells co-cultured with either M1 or M2 microglia was assessed and the exosomes of M2-polarized and unpolarized BV-2 microglia were extracted. Subsequently, we labeled the exosomes with PKH67 and treated GL261 cells with them to investigate the exosome distribution. GL261 cell phenotypes and related protein expression were used to explore the role of M2 microglial exosomes in gliomas. Then a specific miR-7239-3p inhibitor was added to verify miR-7239-3p functions. Finally, the mouse subcutaneous tumorigenic model was used to verify the tumorigenic effect of M2 microglial exosomes in vivo. Our results showed that in gliomas co-cultured with M2 microglia, the expression of the BMAL1 protein was decreased (P < 0.01), while the expression of the CLOCK protein was increased (P < 0.05); opposite results were obtained in gliomas co-cultured with M1 microglia. After treatment with M2 microglial exosomes, the apoptosis of GL261 cells decreased (P < 0.001), while the viability, proliferation, and migration of GL261 cells increased. Increased expression of N-cadherin and Vimentin, and decreased E-cadherin expression occurred upon treatment with M2 microglial exosomes. Addition of an miR-7239-3p inhibitor to M2 microglial exosomes reversed these results. In summary, we found that miR-7239-3p in the glioma microenvironment is recruited to glioma cells by exosomes and inhibits Bmal1 expression. M2 microglial exosomes promote the proliferation and migration of gliomas by regulating tumor-related protein expression and reducing apoptosis.

show abstract

A Tissue-Specific Rhythmic Recruitment Pattern of Leukocyte Subsets

Cited by 15 publications

References 41 publications

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV

Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV

Changes in Circadian Rhythms Dysregulate Inflammation in Ageing: Focus on Leukocyte Trafficking

Microglial Exosome miR-7239-3p Promotes Glioma Progression by Regulating Circadian Genes

Contact Info

Product

Resources

About