A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain)

Banerjee, Nirmalya; Langhe, Edmond De

doi:10.1007/bf00269897

Cited by 135 publications

(63 citation statements)

References 8 publications

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“…neste trabalho, não foram observadas grandes diferenças quanto ao número de plântulas obtidas por explante inicial das cultivares de grupos genômicos triplóides aaa e aaB, embora Sandoval et al (1991) Considera-se que as médias de brotos/rizoma de 2,00 a 4,75 são aceitáveis, uma vez que vuylsteke e de langhe (1985), Banerjee e de langhe (1985), Wong (1986), Oliveira et al (2001) obtiveram taxa de multiplicação de bananeira que variaram entre duas e dez plântulas por subcultivo a cada quatro ou cinco semanas. no entanto, segundo Jarret et al (1985), podem ser obtidas até 31 plântulas por subcultivo.…”

Section: Resultsunclassified

“…uma série de trabalhos tem sido desenvolvida visando estabelecer protocolos seguros para a multiplicação in vitro de cultivares de bananeira (Cronauer e Krikorian 1984;Hwang et al 1984;Banerjee e de langhe 1985;Wong 1986; angarita e Perea 1991; Sandoval et al 1991). esses trabalhos demonstraram que para cada genótipo devem ser realizadas modificações nos protocolos de micropropagação quanto ao tipo de explante, concentração de componentes do meio de cultura e as condições físicas do ambiente.…”

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Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp.)

Oliveira

Lemos

Miranda³

et al. 2011

Acta Amaz.

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Resumo a banana (Musa spp.) é uma das frutas mais consumidas no mundo, e amplamente cultivada no Brasil, porém doenças como as sigatokas, negra e amarela, vêm reduzindo a sua produção. a disponibilização imediata de novas cultivares resistentes às principais doenças é limitada pela propagação convencional. a micropropagação é uma alternativa para a produção de mudas com qualidade fitossanitária e vegetativa, mas apresenta fatores que dificultam sua aplicação como a contaminação por fungos e bactérias, associada à oxidação dos explantes. O objetivo desse trabalho foi adaptar e/ou otimizar as etapas do processo de micropropagação para diferentes cultivares de bananeira, por meio do controle de oxidação, contaminação, e multiplicação de brotos, sendo utilizadas as cultivares Caipira (aaa), BrS Caprichosa (aaaB), Pacovan Ken (aaaB), Preciosa (aaaB), Pv 03-76 (aaaB), Thap maeo (aaB). no estudo foram utilizados o antibiótico sulfato de estreptomicina e o fungicida Opera® (BaSF) visando reduzir a contaminação in vitro provocada por bactérias e fungos, além do anti-oxidante PvP (polivinilpirrolidona) para controlar a oxidação. Houve redução da contaminação com uso do sulfato de estreptomicina à concentração de 100 mg l -1 e da oxidação com PvP a 4 g l -1. na fase de multiplicação de brotos, as cultivares apresentaram médias que variaram de 1,90 a 4,75 brotos/explante. a cultivar caipira (aaa) destacou-se das demais com a maior taxa de multiplicação de brotos após três subcultivos, média de 41,50 brotos por rizoma. PalavRas-Chave: banana, oxidação, contaminação, multiplicação in vitro. Establishment and in vitro multiplication of banana (Musa spp.) cultivars with the use of PVP (Polyvinylpyrrolidone) abstRaCtThe banana (Musa spp) is one of the most consumed fruits in the world and is widely consumed in Brazil, but diseases such as yellow and black sigatoka have been reducing its production. The immediate availability of new cultivars resistant to major diseases is limited by conventional propagation. The micropropagation, is an alternative for the production of seedlings with phytosanitarium and vegetative quality, but presents factors that difficult its application such as contamination by fungi and bacteria, associated with oxidation of the explants. The objective of this work was to adapt and/or optimize the stages of the micropropagation of banana cultivars, through the control of oxidation, contamination, and multiplication of shoots. The cultivars used Caipira (aaa), BrS Caprichosa (aaaB), Pacovan Ken (aaaB), Preciosa (aaaB), Pv 03-76 (aaaB), Thap maeo (aaB) were subjected to different micropropagation stages. in the study we used the streptomycin sulfate antibiotic and fungicide Opera® (BaSF) to reduce contamination in vitro caused by bacteria and fungi, besides the anti-oxidant PvP (polivinilpirrolidona) to control the oxidation. We found contamination reduction with the use of streptomycin sulfate in the concentration of 100 mg l -1 and of oxidation with PvP at 4 g l -1 . at the stage of multiplication of shoots...

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Section: Resultsunclassified

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Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp.)

Oliveira

Lemos

Miranda³

et al. 2011

Acta Amaz.

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“…Micropropagation techniques were developed during the past two decades and are now well established (Banerjee and De Langhe 1985;Cronauer and Krikorian 1984;Israeli et al 1995;Vuylsteke 1989). Tissue culture labs supply the regular replanting needs of the large export banana plantations (Smith and Drew 1990a).…”

Section: Micropropagationmentioning

confidence: 99%

“…Shoot-tip cultures maintained at lower temperatures (15-18°C) have been used for the slow-growth storage of Musa germplasm (Banerjee and De Langhe 1985;Van den houwe et al 1995). A simple technique was recently developed for cryopreservation of meristem cultures, which involves preculture on high-sucrose medium followed by rapid freezing (Panis et al 1996).…”

Section: Germplasm Exchange and Conservationmentioning

confidence: 99%

Perspectives on the application of biotechnology to assist the genetic enhancement of plantain and banana (Musa spp.)

Crouch¹

1998

Electron. J. Biotechnol.

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Bananas and plantains (Musa spp.) are the most important tropical fruit crops. They form an integral component of the farming systems in the humid agroecological zones of the tropics. A broad array of applied cell and molecular techniques are increasingly being used worldwide to facilitate and enhance the handling and improvement of plantain and banana germplasm. Tissue culture is used for germplasm exchange, conservation and rapid multiplication, while in vitro seed germination (based on embryo culture or rescue) plays a critical role in generating hybrid plants. DNA marker systems have been developed in Musa to assist germplasm management, selection within the breeding pool or gene introgression from wild species, and for disease diagnosis. Likewise, genetic transformation using the particle gun method or through Agrobacterium co-cultivation shows potential for the genetic betterment of the crop. This article discusses the applications of biotechnology for the genetic enhancement of banana and plantain. It highlights current advances by research teams across the world and reviews progress in molecular breeding of Musa by the International Institute of Tropical Agriculture and its collaborators.Plantains and bananas (Musa spp.) are staple foods for rural and urban consumers in the humid tropics and an important source of rural income, particularly in some locations where smallholders produce them in compound or home gardens. World Musa production is around 85.5 million tonnes annually (FAO 1998), of which bananas cultivated for the export trade account for only 10%. Hence, fruit harvested from bananas and plantains are important components of food security in the tropical world, and provide income to the farming community through local and international trade.Most banana cultivars and all plantain landraces have 33 chromosomes (2n = 3x). These triploid genotypes are virtually or completely sterile and develop their fruit by vegetative parthenocarpy. Diploid landraces and tetraploid cultivars (mostly artificial hybrids) are also cultivated. The evolution of Musa cultivars from their wild species (M. acuminata Colla. and M. balbisiana Colla.), and their dispersal from the southeast Asian centre of origin to the new secondary centres of diversity, has been documented in detail (Simmonds 1962). For example, recurrent somatic mutations followed by human selection for their tasty fruit led to great phenotypic diversity amongst plantain landraces in the humid lowlands of West Africa, and amongst beer and cooking bananas in the highlands of East Africa.Many pests and diseases have significantly affected Musa cultivation. More virulent fungal strains of Fusarium oxysporum Schlecht f. sp. cubense (E.F. Smith) Snyd & Hans (Panama disease or wilt), and Mycosphaerella fijiensis Morelet (black Sigatoka) have spread dramatically during the past 20 years. As a consequence of these threats to Musa cultivation, there has been renewed interest in Musa breeding programs. Although some programs have addressed improvement of export ...

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“…uniformity of plants, freedom from viral diseases, plants give an early yield, easy for bulk transportation, faster turnover/mass production of plants all the year round. However, during field establishment, the tissue culture plants require a higher level of care than conventional planting material (Banarjee and De Langhe, 1985).…”

Section: Introductionmentioning

confidence: 99%

A Fast Protocol for in vitro Cloning of Banana (Musa acuminata) cv. Amritpani

Maharana¹,

Beura²,

Munsi³

2017

Int.J.Curr.Microbiol.App.Sci

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A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain)

Cited by 135 publications

References 8 publications

Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp.)

Estabelecimento e multiplicação in vitro de brotos no processo de micropropagação de cultivares de bananeira (Musa spp.)

Perspectives on the application of biotechnology to assist the genetic enhancement of plantain and banana (Musa spp.)

A Fast Protocol for in vitro Cloning of Banana (Musa acuminata) cv. Amritpani

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