A time-lapse evaluation of conventional slow freezing and vitrification of PN-stage embryos

Meintjes, M.; Purcell, S.; Chantilis, S.J.; Navarrete, G.; Tilley, B.; Lee, K.L.; Thomas, M.; Qin, Y.; Davidson, Katharine; Mehta, Rajvi; Guerami, A.

doi:10.1016/j.fertnstert.2015.07.584

Cited by 1 publication

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“…[2, 18] Besides, the conventional slow freezing approach requires a commercially available programmable freezer or a cryogenic refrigerator with a lengthy (up to hours) cooling process, [16, 20] and after cooling, the samples must be transferred into liquid nitrogen (LN 2 ) for long-term storage. [21] These factors make it uneconomic, time-consuming, and complicated. [5] Vitreous cryopreservation as an emerging strategy, is regarded to be safer and more reliable for cell preservation when compared with the conventional slowing freezing method.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel Encapsulation Facilitates Rapid‐Cooling Cryopreservation of Stem Cell‐Laden Core–Shell Microcapsules as Cell–Biomaterial Constructs

Zhao

Liu

Zhu

et al. 2017

Adv Healthcare Materials

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Core-shell structured stem cell microencapsulation in hydrogel has wide applications in tissue engineering, regenerative medicine, and cell-based therapies because it offers an ideal immunoisolative microenvironment for cell delivery and 3D culture and differentiation. Long-term storage of such microcapsules as cell-biomaterial constructs by cryopreservation is an enabling technology for their wide distribution and ready availability for clinical transplantation. However, most of the existing studies focused on cryopreservation of separated cells or cells in microcapsules without a core-shell structure (i.e., hydrogel beads). The goal of this study is to achieve cryopreservation of stem cells encapsulated in core-shell microcapsules as cell-biomaterial constructs or biocomposites. To this end, a capillary microfluidics-based core-shell alginate hydrogel encapsulation technology is developed to facilitate cryopreservation of porcine adipose-derived stem cells (pADSCs) laden microcapsules with very low concentration (2 mol L−1) of cell membrane penetrating cryoprotective agents (CPAs) by suppressing ice formation. This may provide a low-CPA and cost-effective approach for vitreous cryopreservation of “ready-to-use” stem cell-biomaterial constructs, facilitating their off-the-shelf availability and widespread applications.

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