A time-dependently regulated gene network reveals that Aspergillus protease affects mitochondrial metabolism and airway epithelial cell barrier function via mitochondrial oxidants

Kim, Bu-Yeo; Kim, Yun Hee; Kim, Taesoo; Ji, Kon-Young; Shin, In‐Sik; Lee, Joo Young; Song, Kwang Hoon

doi:10.1016/j.freeradbiomed.2022.04.013

Cited by 5 publications

(3 citation statements)

References 65 publications

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“…A. avus could produce a variety of mycotoxins, such as a atoxins, cyclopiazonic acid and 3-nitropropionic acid, that have strong carcinogenic, teratogenic, and organ necrosis properties that threaten the food safety of agricultural products [36,37]. On the other hand, this kind of fungus may cause human invasive aspergillosis or allergenic problems [38,39].…”

Section: Discussionmentioning

confidence: 99%

Aspergillus flavus as an Entomopathogen Infecting Diaphania indica and Control Efficacy Across Different Developmental Stages

Askar,

Chen,

Borham

et al. 2024

Preprint

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Diaphania indica (Lepidoptera: Crambidae) is one of the most important pests infesting many cucurbitaceous vegetables. During the rearing of insect eggs, we observed a fungal infection of these insect eggs. The fungus produces aflatoxins which are considered secondary polyketide metabolites, which cause the death of pests. Therefore, this work aimed to isolate and identify this fungus by amplifying the internal transcribed spacer (ITS) region of the rDNA, as well as evaluating the efficiency of this fungus in control. Aspergillus flavus, 'PP125556,' showcased robust pathogenicity against a range of D. indica pests. The results showed that colonies of 'PP125556' cultivated on potato dextrose agar (PDA) exhibited distinctive morphological characteristics, transitioning from pristine white to verdant green. Bioassays demonstrated concentration-dependent mortality rates of D. indica larvae and adults when exposed to varying concentrations of 'PP125556' conidia, with the highest concentration (1x109 conidia/ml) inducing significant death with the highest mortality (53.06% for eggs, 70.57% for larvae, and 86.65% for adults). Furthermore, examination under a stereomicroscope revealed conspicuous external symptoms in infected larvae, including reduced mobility, darkened body pigmentation, and the emergence of white hyphae, indicative of mortality. Additionally, infected eggs exhibited inhibited hatching and the emergence of green hyphae, while infected adults displayed mortality and white hyphae colonization, underscoring the potent biocontrol efficacy of A. flavus 'PP125556' against D. indica across diverse developmental stages.

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Section: Discussionmentioning

confidence: 99%

Aspergillus flavus as an Entomopathogen Infecting Diaphania indica and Control Efficacy Across Different Developmental Stages

Askar,

Chen,

Borham

et al. 2024

Preprint

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“…Each group consisted of 26-week-old mice fed either ND or HFD, with seven mice per group. Asthma was induced by intranasal injection of a protease from Aspergillus oryzae (Asp; Sigma-Aldrich, St. Louis, MO, USA), fungi closely associated with allergic diseases in humans [ 19 , 20 ], mixed with OVA (Sigma-Aldrich). When inhaled with OVA, the active protease stabilizes Th2 effector commitment to OVA and perpetuates allergic lung inflammation to induce allergic immunity [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

The absence of thioredoxin-interacting protein in alveolar cells exacerbates asthma during obesity

Jeong,

Kim,

Kim

et al. 2024

Redox Biology

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“…An increased production of mitochondrial reactive oxygen species (ROS) at the time of exposure to Asp. proteases also contributed to the epithelial cell barrier function being damaged, leading to pathological changes [ 129 ].…”

Section: Allergen Serine Proteases and Airway Epithelial Barrier Dysf...mentioning

confidence: 99%

Airborne indoor allergen serine proteases and their contribution to sensitisation and activation of innate immunity in allergic airway disease

Ouyang,

Reihill,

Douglas

et al. 2024

Eur Respir Rev

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Common airborne allergens (pollen, animal dander and those from fungi and insects) are the main triggers of type I allergic disorder in the respiratory system and are associated with allergic rhinitis, allergic asthma, as well as immunoglobulin E (IgE)-mediated allergic bronchopulmonary aspergillosis. These allergens promote IgE crosslinking, vasodilation, infiltration of inflammatory cells, mucosal barrier dysfunction, extracellular matrix deposition and smooth muscle spasm, which collectively cause remodelling of the airways. Fungus and insect (house dust mite and cockroaches) indoor allergens are particularly rich in proteases. Indeed, more than 40 different types of aeroallergen proteases, which have both IgE-neutralising and tissue-destructive activities, have been documented in the Allergen Nomenclature database. Of all the inhaled protease allergens, 85% are classed as serine protease activities and include trypsin-like, chymotrypsin-like and collagenolytic serine proteases. In this article, we review and compare the allergenicity and proteolytic effect of allergen serine proteases as listed in the Allergen Nomenclature and MEROPS databases and highlight their contribution to allergic sensitisation, disruption of the epithelial barrier and activation of innate immunity in allergic airways disease. The utility of small-molecule inhibitors of allergen serine proteases as a potential treatment strategy for allergic airways disease will also be discussed.

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A time-dependently regulated gene network reveals that Aspergillus protease affects mitochondrial metabolism and airway epithelial cell barrier function via mitochondrial oxidants

Cited by 5 publications

References 65 publications

Aspergillus flavus as an Entomopathogen Infecting Diaphania indica and Control Efficacy Across Different Developmental Stages

Aspergillus flavus as an Entomopathogen Infecting Diaphania indica and Control Efficacy Across Different Developmental Stages

The absence of thioredoxin-interacting protein in alveolar cells exacerbates asthma during obesity

Airborne indoor allergen serine proteases and their contribution to sensitisation and activation of innate immunity in allergic airway disease

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