A Time Course Study on STR Profiles Derived from Human Bone, Muscle and Bone Marrow

Frank, William E.; Llewellyn, Barbara

doi:10.1520/jfs14551j

Cited by 3 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A differential extraction procedure (23) was used to isolate epithelial cell DNA and sperm cell DNA for these amplifications. Consistent with studies of different tissue types (24), no differences were noted between blood standard DNA profiles and the corresponding epithelial cell DNA profile defined by the AmpFᐉSTR Profiler Plus PCR Amplification Kit.…”

Section: Discussionsupporting

confidence: 80%

Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

Frank¹,

Llewellyn²,

Fish³

et al. 2001

Journal of Forensic Sciences

Self Cite

View full text Add to dashboard Cite

According to TWGDAM guideline 4.5 (1), prior to implementing a new DNA analysis procedure or an existing DNA analysis procedure developed by another laboratory, the forensic laboratory must first demonstrate reliability of the procedure inhouse. Seven phases were designed to validate the use of the AmpFᐉSTR Profiler Plus PCR Amplification Kit, as well as the PE Applied Biosystems 310 Genetic Analyzer. This report summarizes the results obtained for each of the seven phases of the validation study which included the following evaluations: polymer, reproducibility, sensitivity, stutter, precision, mixtures and nonprobative casework.

show abstract

Section: Discussionsupporting

confidence: 80%

Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

Frank¹,

Llewellyn²,

Fish³

et al. 2001

Journal of Forensic Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Polymerase chain reaction analysis of STR loci is a simple and accurate way to genotype individuals using a limited amount of DNA, 7,12,13 and laser‐based microdissection efficiently procures a pure target cell population 14 . Therefore, we were able to isolate neutrophils from histological sections of the placenta and successfully perform the genotypical analysis of those neutrophils from different anatomical compartments of the placenta.…”

Section: Discussionmentioning

confidence: 99%

Chorionic plate vessels as an origin of amniotic fluid neutrophils

Lee¹,

Kim

Hwang³

et al. 2004

Pathology International

View full text Add to dashboard Cite

The present study was conducted to investigate the potential anatomical source of amniotic fluid neutrophils. Microdissection of neutrophils from the chorioamnion of the fetal membranes and the amnion of the chorionic plates of 10 preterm placentas with acute chorioamnionitis was performed and the genotypes of the neutrophils were compared with those of the mother and fetus using polymerase chain reaction of nine autosomal STR loci. In separate analyses, we reviewed eight cases of fetal autopsies with increased amniotic fluid neutrophils for the presence of neutrophils in the alveoli, and also analyzed the relationship between the amniotic fluid white blood cell (WBC) count and the histological pattern of placental inflammation. The genotypes of all of the neutrophils found in the chorioamnion of the fetal membrane matched those of the mother (n = 10). The genotypes of neutrophils found in the chorionic plate were of mixed maternal and fetal origin (n = 4). In the autopsy series of the fetuses with amniotic fluid WBC (n = 8), only five cases showed neutrophils in the alveolar space, while all the placentas had chorioamnionitis. There was no significant difference in amniotic fluid WBC count between the cases with or without acute membranitis, while among the cases with placental inflammation, those with inflammation of the chorionic plate had a significantly higher amniotic fluid WBC count than both the membranitis-only cases (P < 0.001) and the membranitis and funisitis cases (P < 0.05). These results imply that fetal vasculature at the chorionic plate is the main source of amniotic fluid neutrophils, especially in the cases without funisitis.

show abstract

“…BM may therefore be a relevant alternative tissue, especially when remains are skeletonized, bloodless or in extreme putrefaction. BM is already used in forensic science for genetic analysis [4], to estimate postmortem interval [5][6][7][8], in the diatom test for diagnosis of drowning [9,10] and in histopathology to establish certain malignancies postmortem [11][12][13]. In forensic toxicology, the first report of BM analysis was in 1943, to document the kinetics of alcohol [14].…”

Section: Introductionmentioning

confidence: 99%

State-of-the-art of bone marrow analysis in forensic toxicology: a review

et al. 2010

View full text Add to dashboard Cite

Although blood is the reference medium in the field of forensic toxicology, alternative matrices are required in case of limited, unavailable or unusable blood samples. The present review investigated the suitability of bone marrow (BM) as an alternative matrix to characterize xenobiotic consumption and its influence on the occurrence of death. Basic data on BM physiology are reported in order to highlight the specificities of this matrix and their analytical and toxicokinetic consequences. A review of case reports, animal and human studies involving BM sample analysis focuses on the various parameters of interpretation of toxicological results: analytic limits, sampling location, pharmacokinetics, blood/BM concentration correlation, stability and postmortem redistribution. Tables summarizing the analytical conditions and quantification of 45 compounds from BM samples provide a useful tool for toxicologists. A specific section devoted to ethanol shows that, despite successful quantification, interpretation is highly dependent on postmortem interval. In conclusion, BM is an interesting alternative matrix, and further experimental data and validated assays are required to confirm its great potential relevance in forensic toxicology.

show abstract

A Time Course Study on STR Profiles Derived from Human Bone, Muscle and Bone Marrow

Cited by 3 publications

References 12 publications

Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

Validation of the AmpFℓSTR™ Profiler Plus PCR Amplification Kit for Use in Forensic Casework

Chorionic plate vessels as an origin of amniotic fluid neutrophils

State-of-the-art of bone marrow analysis in forensic toxicology: a review

Contact Info

Product

Resources

About