A time course study of cadmium effect on membrane permeability of single human bladder cancer cells using scanning electrochemical microscopy

Li, Michelle S. M.; Filice, Fraser P.; Ding, Zhifeng

doi:10.1016/j.jinorgbio.2014.02.009

Cited by 33 publications

(35 citation statements)

References 46 publications

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“…Cell death is a stress response to Cd in plants. The excessive accumulation of reactive oxygen species (ROS) caused by Cd exacerbates DNA damage and increases cell membrane permeability and the loss of transmembrane potential (Li et al, 2003;Michelle et al, 2014;Li et al, 2017;Huybrechts et al, 2019;Wang et al, 2019b). Vacuolar rupture is a unique and important factor causing cell death in higher plants, which is mediated by vacuolar processing enzyme (VPE) (Yamada et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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Excessive cadmium (Cd) damages plants by causing cell death. The present study discusses the function of natural resistance-associated macrophage protein (NRAMP) on cell death caused by Cd in Malus hupehensis. MhNRAMP1 was isolated from M. hupehensis roots, and its protein was located in the cell membrane as a transmembrane protein characterized by hydrophobicity. MhNRAMP1 expression in the roots was induced by Cd stress and calcium (Ca) deficiency. MhNRAMP1 overexpression increased Cd concentration in yeasts and enhanced their sensitivity to Cd. Phenotypic comparisons of plants under Cd stress revealed that the growth of transgenic tobacco and apple calli overexpressing MhNRAMP1 was worse than that of the wild type (WT). The Cd 2+ influx of transgenic tobacco roots and apple calli was higher, and the recovery time of the Cd 2+ influx to a stable state in transgenic apple calli was longer than that of the WT. Cd accumulation and the percentage of apoptotic cells in transgenic lines were higher. Correspondingly, the caspase-1-like and vacuolar processing enzyme (VPE) activities and MdVPEg expression were higher in transgenic apple calli, but the expression levels of genes that inhibit cell death were lower than those in the WT under Cd stress. Moreover, the Cd translocation from the roots to leaves was increased after MhNRAMP1 overexpression, but the Cd translocation from the leaves to seeds was not affected. These results suggest that MhNRMAP1 exacerbated Cd-induced cell death, which was accomplished by mediating Cd 2+ uptake and accumulation, as well as stimulating VPE.

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Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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“…8,35,45,52,53 We have previously used ferrocenemethanol (FcCH 2 OH) as a molecular probe to investigate membrane permeability changes in single live human bladder cancer (T24) cells under Cd 2+ and cisplatin stimulations. 8,9,33,53 While relative changes in permeability can be observed, the molecular probe itself is permeable to the cell. It becomes attractive to select a sensing agent that will display strong contrast between control (impermeable) and stressed cells (permeable).…”

Section: ■ Introductionmentioning

confidence: 99%

Probing Cd²⁺-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy

Filice

Henderson

et al. 2016

J. Phys. Chem. C

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Heavy metal cytotoxicity has become a mainstay in scientific research due to the vast range of detrimental effects on living organisms. Cd 2+ in particular can activate a wide range of physiological pathways that can cause significant increases in oxidative stress, cancer formation, or cellular death. This is partly due to its ability to bioaccumulate in the body with a long half-life for excretion. Here, we used hydrophilic redox mediators, ferrocenecarboxylate, 1′1-ferrocene dicarboxylate and hexaamineruthenium, as molecular probes in scanning electrochemical microscopy (SECM) to determine the membrane permeability in single live human bladder cancer (T24) cells affected by Cd 2+ . Hydrophilic and charged sensing agents should be impermeable to the cells. However, when T24 cells were treated with 25 μM Cd 2+ (t ≤ 6 h), the membrane permeability to these sensing agents can be induced to approximately 7.0 × 10 −5 m/s. For acute mM Cd 2+ exposure, the membrane permeability can be induced to 2.0 × 10 −4 m/s within an hour. This increase in membrane permeability is indicative of the loss in membrane integrity, where species can now enter the cell through nonspecific diffusion channels, pore formation, or structural collapse of the membrane. Using the MTT proliferation assay, we were able to confirm that the low dosage Cd 2+ treatment had very minor effect on cellular viability, while the acute treatments decreased viability to 68%. Our SECM results coupled with the MTT assays show the sufficient permeation of these sensors and compromised cell viability. These findings by means of SECM demonstrate that hydrophilic redox mediators are ideal in sensing the effects of toxins, which may induce permeability through detrimental effects on the membrane integrity of the cells.

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“…Exotic targets for biological SECM studies were salt glands on the leaf of the halophyte Cynodon dactylon (L.) [70] and chloroplasts and their thylakoid membranes for which local photosynthetic flux measurements have been attempted [71]. SECM study entries into the literature databases that underline well the wide applicability of scanning electrochemical probe-based cellular analysis are examinations of the differentiation status of embryonic stem cells [72], evaluation of the respiration activity of blastocysts [73], monitoring intracellular enzyme activity of HeLa cells [74], analysis of beat fluctuations and oxygen consumption of cardiomyocytes [75], characterization of Ag + toxicity on fibroblast cells [76], inspection of the multi-drug resistance of adenocarcinoma cervical cancer cells [77], the detection of reactive oxygen species in prostate cancer cells [78], the creation of insulin release profiles of single pancreatic islets [79] and the disclosure of Cd 2+ -induced variability in the permeation properties of human bladder cancer cells [80,81]. Recent activities also addressed topics such as SECM-based inspections of heavy metal (chromium) cell toxicity [82,83], quantitative detection of protein markers for lung cancer [84], real-time monitoring of metabolic crosstalk between neighbouring bacterial cells [85], spatial mapping of molecular uptake of chemical species at living cells [59] and the pH dependence of yeast cell viability and metabolism [66].…”

Section: Scanning Electrochemical Microscopy-based Live Cell Imaging: Examples Of Accomplishments From 2012 To Datementioning

confidence: 99%

Biological imaging with scanning electrochemical microscopy

2018

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Scanning electrochemical microscopy (SECM) is a powerful and versatile technique for visualizing the local electrochemical activity of a surface as an ultramicroelectrode tip is moved towards or over a sample of interest using precise positioning systems. In comparison with other scanning probe techniques, SECM not only enables topographical surface mapping but also gathers chemical information with high spatial resolution. Considerable progress has been made in the analysis of biological samples, including living cells and immobilized biomacromolecules such as enzymes, antibodies and DNA fragments. Moreover, combinations of SECM with complementary analytical tools broadened its applicability and facilitated multi-functional analysis with extended life science capabilities. The aim of this review is to present a brief topical overview on recent applications of biological SECM, with particular emphasis on important technical improvements of this surface imaging technique, recommended applications and future trends.

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A time course study of cadmium effect on membrane permeability of single human bladder cancer cells using scanning electrochemical microscopy

Cited by 33 publications

References 46 publications

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

Probing Cd²⁺-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy

Biological imaging with scanning electrochemical microscopy

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A time course study of cadmium effect on membrane permeability of single human bladder cancer cells using scanning electrochemical microscopy

Cited by 33 publications

References 46 publications

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

Probing Cd2+-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy

Biological imaging with scanning electrochemical microscopy

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Probing Cd²⁺-Stressed Live Cell Membrane Permeability with Various Redox Mediators in Scanning Electrochemical Microscopy