A three-way junction aptasensor for lysozyme detection

Xia, Yuxing; Gan, Shuoqiu; Xu, Qiu; Qiu, Xiaowen; Gao, Peiyi; Huang, Shasheng

doi:10.1016/j.bios.2012.07.053

Cited by 40 publications

(20 citation statements)

References 24 publications

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“…click chemistry between azide-modified gold particles and alkyne-terminated aptamers ( Figure 2D) [60] 5. electrostatic interactions between the negatively-charged phosphate backbone of the aptamer and positively-charged materials ( Figure 2E), such as polypyrrole, Fe 2 O 3 and ferrocene-appended poly(ethyleneimine) (Fc-PEI) in a layer-by-layer approach or amine-rich films of plasma-polymerized propargylamine in a Cu 2 O@rGO@PpPG-modified gold electrode [74,78,84,88] 6. affinity binding based on biotin-avidin [80] or host-guest interactions [89] (Figure 2F) 7. hybridization to a partially complementary DNA strand, previously immobilized on the electrode surface ( Figure 2G) [96] Chemosensors 2016, 4, 10 7 of 21 The majority of electrochemical aptasensors for lysozyme used thiolated aptamers on gold electrode, that is following Strategy (C) [46,62,87,91,94]. To increase the aptamer loading, a popular approach relies on modifying the electrodes with Au nanoparticles [47,76].…”

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confidence: 99%

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

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confidence: 99%

Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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“…4), proving the good selectivity of the aptasensor and its potential use for the detection of lysozyme in food matrices. SPR, graphene coated interface 0.5 [10] SWV/three-way junction DNA structure, Fc-tagged cDNA 0.2 [11] EIS/MWCNT-SPE 862 [12] EIS/chitosan-graphene oxide 28.5 [13] SWV/Au NP amplification 2.0 × 10 −5 [14] a Abbreviations: SWV: square wave voltammetry; EIS: electrochemical impedance spectroscopy; MWCNT: multi wall carbon nanotubes; SPE: screen-printed electrode; NP: nanoparticle.…”

Section: Sensor Selectivitymentioning

confidence: 99%

“…the standard method provided by OIV in 2010) [6] or on immunosensing by ELISA [7]. Various sensors have been described also as alternatives to these classic analytical methods, based mainly on electrochemical and optical methods [8][9][10][11][12][13][14]. Some allowed reaching very low detection limits, down to 10 −14 M [14].…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of lysozyme in wines using an aptamer based biosensor and SPR detection

Mihai¹,

Vezeanu²,

Polonschii³

et al. 2015

Sensors and Actuators B: Chemical

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“…After binding to target molecule, the target-aptamer complex is liberated into the solution, leading to detectable signals. Target-induced dissociation/displacement methods are further classified into “signal-off” mode [77], “signal-on” mode [78] and “label-free” mode [79]. …”

Section: Design Of Sensitive Biosensors For Allergen Based On Scrementioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Disposable Screen-Printed Electrodes for Detection of Food Allergens

Vasilescu

Nunes

Hayat

et al. 2016

Sensors

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Food allergens are proteins from nuts and tree nuts, fish, shellfish, wheat, soy, eggs or milk which trigger severe adverse reactions in the human body, involving IgE-type antibodies. Sensitive detection of allergens in a large variety of food matrices has become increasingly important considering the emergence of functional foods and new food manufacturing technologies. For example, proteins such as casein from milk or lysozyme and ovalbumin from eggs are sometimes used as fining agents in the wine industry. Nonetheless, allergen detection in processed foods is a challenging endeavor, as allergen proteins are degraded during food processing steps involving heating or fermentation. Detection of food allergens was primarily achieved via Enzyme-Linked Immuno Assay (ELISA) or by chromatographic methods. With the advent of biosensors, electrochemical affinity-based biosensors such as those incorporating antibodies and aptamers as biorecognition elements were also reported in the literature. In this review paper, we highlight the success achieved in the design of electrochemical affinity biosensors based on disposable screen-printed electrodes towards detection of protein allergens. We will discuss the analytical figures of merit for various disposable screen-printed affinity sensors in relation to methodologies employed for immobilization of bioreceptors on transducer surface.

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A three-way junction aptasensor for lysozyme detection

Cited by 40 publications

References 24 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

Label-free detection of lysozyme in wines using an aptamer based biosensor and SPR detection

Electrochemical Affinity Biosensors Based on Disposable Screen-Printed Electrodes for Detection of Food Allergens

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