A three-layer diffusion-cell to examine bio-enhanced dissolution of chloroethene dense non-aqueous phase liquid

Philips, Jo; Springael, Dirk; Smolders, Erik

doi:10.1016/j.chemosphere.2011.02.012

Cited by 15 publications

(31 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anaerobic medium identical to that used for growing the cultures was applied and the same type of sand was used as described by Philips et al . (). The bottom DNAPL layers contained 1 mL of pure TCE droplets.…”

Section: Methodsmentioning

confidence: 97%

“…Incubation was performed at 20 ± 1 °C. The processes in the diffusion‐cells were expected to be in steady state after about 20 days (Philips et al ., ). At that time, pore water samples were taken from the sampling ports of the diffusion‐cells for DNA analysis.…”

Section: Methodsmentioning

confidence: 99%

“…At the end of the experiment (after 35 days), the sand layer of this diffusion‐cell was sliced as described by Philips et al . (), and layers of about 5 mm were sampled for DNA analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Philips et al . () recently developed a three‐layer diffusion‐cell set‐up to measure abiotic and biotic conditions in the vicinity of a DNAPL with a 5‐mm resolution. This diffusion‐cell set‐up was used in this study to examine the distribution of both dechlorinating and fermentative microorganisms in relation to the distance from a TCE DNAPL.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Distribution of a dechlorinating community in relation to the distance from a trichloroethene dense nonaqueous phase liquid in a model aquifer

Philips

Hamels

Smolders

et al. 2012

FEMS Microbiol Ecol

Self Cite

View full text Add to dashboard Cite

The toxicity of trichloroethene (TCE) likely restricts microbial activity in close vicinity of a TCE dense nonaqueous phase liquid (DNAPL). This study examined the distribution of a dechlorinating community in relation to the distance from a TCE DNAPL using a diffusion-cell set-up. Subcultures of the KB-1(™) culture dechlorinating TCE to cis-dichloroethene and grown with either formate or lactate as electron donor were used to inoculate the diffusion-cells. 16S rRNA gene clone library analysis showed that both inocula consisted of dechlorinating bacteria similar to Geobacter lovleyi SZ and fermentative microorganisms related to Clostridium and Clostridiales. qPCR and RFLP analyses of pore water and sand samples showed a stratified microbial community composition in the diffusion-cells. Geobacter dominated where TCE was present, that is, in the lower 3 cm of the 5.5-cm-thick sand layer. Even at 0.5 cm distance from the DNAPL layer, Geobacter densities were two orders of magnitude higher than at inoculation, despite the expected TCE toxicity. In the upper 2.5 cm of the sand layer, where TCE was depleted, apparently fermenting populations prevailed, corresponding to Clostridium in some diffusion-cells. This analysis demonstrates that the microbial community composition in a source zone is related to the distance from the DNAPL.

show abstract

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distribution of a dechlorinating community in relation to the distance from a trichloroethene dense nonaqueous phase liquid in a model aquifer

Philips

Hamels

Smolders

et al. 2012

FEMS Microbiol Ecol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Carr et al, 2000;Yang and McCarty, 2000;Cope and Hughes, 2001;Sleep et al, 2006;Amos et al, 2008Amos et al, , 2009Schaefer et al, 2010;Philips et al, 2011). This variability in dissolution enhancement has been attributed to several factors, including differing microbial populations, electron donor limitations, competitive and threshold inhibitions, and competitor populations (e.g., methanogens), as well as changing flow conditions due to gas formation, microbial growth, and experimental design.…”

Section: Introductionmentioning

confidence: 99%

Microbially enhanced dissolution and reductive dechlorination of PCE by a mixed culture: Model validation and sensitivity analysis

Chen¹,

Abriola²,

Amos³

et al. 2013

Journal of Contaminant Hydrology

View full text Add to dashboard Cite

Reductive dechlorination catalyzed by organohalide-respiring bacteria is often considered for remediation of non-aqueous phase liquid (NAPL) source zones due to cost savings, ease of implementation, regulatory acceptance, and sustainability. Despite knowledge of the key dechlorinators, an understanding of the processes and factors that control NAPL dissolution rates and detoxification (i.e., ethene formation) is lacking. A recent column study demonstrated a 5-fold cumulative enhancement in tetrachloroethene (PCE) dissolution and ethene formation to ethene, as well as column length and flow rate (i.e., column residence time). If cis-DCE 3 inhibition was neglected, the model over-predicted ethene production nine fold, while reductions in residence time (i.e., a two-fold decrease in column length or two-fold increase in flow rate) resulted in a more than 70% decline in ethene production. These results demonstrate that spatial and temporal microbial community dynamics and activity must be understood to model, predict, and manage bioenhanced NAPL dissolution.

show abstract

Field Evidence of Dissolution and Degradation Rates Enhancement During ISCR and ENA Treatments of Chlorinated Solvents

Barnier

Palmier²,

Atteia

2013

Remediation Journal

View full text Add to dashboard Cite

This article presents field tests comparing two methods of treatment of chlorinated solvents undertaken at the same site. The site is an automobile factory where two chlorinated solvents (CS) plumes were identified. At the first source, in situ chemical reduction (ISCR) was applied, while at the second one, enhanced natural attenuation (ENA) was used. A set of specific multilevel sampling wells were installed approximately 20 m downgradient of the sources to estimate the efficiency of the treatments. The presence of a low-permeability layer (source 1) or a thick oil lens (source 2) in the top part of the aquifer prevented the CS from reaching the bottom of the aquifer. These layers led to difficulties treating the contamination. At the ISCR and ENA treatment zones, the concentrations of tetrachloroethene (PCE) and trichloroethene (TCE) did not change significantly, while the concentration of metabolites (cis-1,2-DCE, vinyl chloride, and ethene) significantly increased 50 to 150 days after treatment. Due to high concentration of CS in the source zone, a mass balance calculation, including chlorine, was possible. It showed that around 1 to 2 percent of the injected products were used to reduce the CS. A detailed analysis and 1D analytical modeling of CS concentrations showed that the treatment led to a large (two to three times) increase in dissolution of the organic phase. This explains why, despite an efficient treatment, the PCE and TCE concentrations remained virtually unchanged. Degradation rates also increased due to the treatment. Due to some differences in the source-zone chemistry, it was not possible to differentiate between the ISCR and ENA efficiencies. O

show abstract

A three-layer diffusion-cell to examine bio-enhanced dissolution of chloroethene dense non-aqueous phase liquid

Cited by 15 publications

References 21 publications

Distribution of a dechlorinating community in relation to the distance from a trichloroethene dense nonaqueous phase liquid in a model aquifer

Distribution of a dechlorinating community in relation to the distance from a trichloroethene dense nonaqueous phase liquid in a model aquifer

Microbially enhanced dissolution and reductive dechlorination of PCE by a mixed culture: Model validation and sensitivity analysis

Field Evidence of Dissolution and Degradation Rates Enhancement During ISCR and ENA Treatments of Chlorinated Solvents

Contact Info

Product

Resources

About