A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells

Kang, Yun Gyeong; Shin, Ji Won; Park, So Hee; Kim, Young Mi; Gu, Seo Rin; Wu, Yanru; Ban, Hun Yeong; Shin, Jung-Woog

doi:10.1007/s10529-015-1952-8

Cited by 14 publications

(8 citation statements)

References 13 publications

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“…Previous studies have confirmed that 3D scaffolds could support the better maintenance of HSCs. However, if use MSCs or ECM protein as feeder layer, their consequences could be improved, because of cellcell interaction between CD34+ cells and feeder cells or ECM [16][17][18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

“…In the previous study, we have shown that PCL nanofiber scaffold coated with fibronectin had a higher number of CD34+ cells and TNCs number than traditional culture medium [15]. In 2016, Kang, et al revealed that the culture of CB derived CD34+ in PCL coated with fibronectin and collagen had a greater number of TNC and CD34+ cells compared to 2D culture system [16].…”

Section: Enhanced Colony Formation Of the Cells Expanded In The Pcl-cmentioning

confidence: 92%

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Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Hadi¹,

Abroun²,

Soleimani³

et al. 2019

Int J Stem Cell Res Ther

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Background: An efficient and practical ex vivo expansion of cord blood hematopoietic stem cells as an alternative source of HSC transplantation is crucial in understanding the potential of HSC transplantation in treating or supportive therapy in a variety of hematologic and non-hematologic disorders. The aim of this study was an ex vivo expansion of cord blood hematopoietic stem cells in a novel threedimensional polycaprolactone nanofiber scaffold coated with collagen. Methods: After 10-day cultured of cord blood CD34+ cells in 2-Dimensional and 3-Dimensional culture system, the evaluation was performed by qRT-PCR, flow cytometry and clonogenicity. Results: 3-Dimensional Polycaprolactone nano-scaffold coated with collagen provided higher total nucleated cells (50-fold vs. 38-fold) and CD34+ cells (20-fold vs. 2.6-fold) (p < 0.05) and compared to 2-Dimensional cell culture and before expansion had higher expression of homing and self-renewal genes and for VLA-4, hTERT and BMI-1 genes were statically significant (p = 0.0001). The expression of myeloid markers in 3-Dimensional scaffold was significantly higher than the 2-Dimensional culture system (p < 0.05). The total colony in 2-Dimensional culture was lower than 3-Dimensional culture medium (p < 0.05). Conclusion: This study demonstrated the synergistic effect between the three-dimensionality of the scaffold and collagen as an extracellular matrix protein, and the potential of this 3-Dimensional Polycaprolactone nanofiber scaffold coated with collagen for ex vivo expansion of HSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Enhanced Colony Formation Of the Cells Expanded In The Pcl-cmentioning

confidence: 92%

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Hadi¹,

Abroun²,

Soleimani³

et al. 2019

Int J Stem Cell Res Ther

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“…In a recent study, Kang et al evaluated the expansion of hematopoietic stem cells seeded onto a three ECM protein‐coated polycaprolactone/hydroxyapatite (PCL/HA)‐composed hierarchical scaffold constructed by ESRP. After three days of cell culture, HSCs cultured in presence of fibronectin showed higher proliferation rates and CD45 + CD34+, CD34 + CD38‐ ratios than collagen‐treated HSCs or controls . Thus, it was demonstrated that ESRP‐fabricated three dimensional hierarchical scaffolds are effective tools for the expansion of HSCs without modifying their phenotype.…”

Section: Physical Approachmentioning

confidence: 98%

Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine

Rana

Ramasamy

Leena

et al. 2016

Biotechnology Progress

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Stem cell-based approaches offer great application potential in tissue engineering and regenerative medicine owing to their ability of sensing the microenvironment and respond accordingly (dynamic behavior). Recently, the combination of nanobiomaterials with stem cells has paved a great way for further exploration. Nanobiomaterials with engineered surfaces could mimic the native microenvironment to which the seeded stem cells could adhere and migrate. Surface functionalized nanobiomaterial-based scaffolds could then be used to regulate or control the cellular functions to culture stem cells and regenerate damaged tissues or organs. Therefore, controlling the interactions between nanobiomaterials and stem cells is a critical factor. However, surface functionalization or modification techniques has provided an alternative approach for tailoring the nanobiomaterials surface in accordance to the physiological surrounding of a living cells; thereby, enhancing the structural and functional properties of the engineered tissues and organs. Currently, there are a variety of methods and technologies available to modify the surface of biomaterials according to the specific cell or tissue properties to be regenerated. This review highlights the trends in surface modification techniques for nanobiomaterials and the biological relevance in stem cell-based tissue engineering and regenerative medicine. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:554-567, 2016.

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“…In another comparative study, fibronectin-coated surface proved to have higher expansion potential in HSC and a higher percentage of CD34 + and CD45 + cells compared with when collagen I coating was used. Probably this is because fibronectin provided a larger surface for cell attachment and stronger adhesion forces in HSC ( Kang et al, 2016 ). A supporting role of MSC with HSC has been proven in many cocultures on various materials.…”

Section: Hematopoietic Stem Cells and Hematopoiesismentioning

confidence: 99%

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

Zmrhal

Svoradová

Bátik

et al. 2022

Front. Cell Dev. Biol.

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Three-dimensional (3D) cell culture is attracting increasing attention today because it can mimic tissue environments and provide more realistic results than do conventional cell cultures. On the other hand, very little attention has been given to using 3D cell cultures in the field of avian cell biology. Although mimicking the bone marrow niche is a classic challenge of mammalian stem cell research, experiments have never been conducted in poultry on preparing in vitro the bone marrow niche. It is well known, however, that all diseases cause immunosuppression and target immune cells and their development. Hematopoietic stem cells (HSC) reside in the bone marrow and constitute a source for immune cells of lymphoid and myeloid origins. Disease prevention and control in poultry are facing new challenges, such as greater use of alternative breeding systems and expanding production of eggs and chicken meat in developing countries. Moreover, the COVID-19 pandemic will draw greater attention to the importance of disease management in poultry because poultry constitutes a rich source of zoonotic diseases. For these reasons, and because they will lead to a better understanding of disease pathogenesis, in vivo HSC niches for studying disease pathogenesis can be valuable tools for developing more effective disease prevention, diagnosis, and control. The main goal of this review is to summarize knowledge about avian hematopoietic cells, HSC niches, avian immunosuppressive diseases, and isolation of HSC, and the main part of the review is dedicated to using 3D cell cultures and their possible use for studying disease pathogenesis with practical examples. Therefore, this review can serve as a practical guide to support further preparation of 3D avian HSC niches to study the pathogenesis of avian diseases.

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A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells

Abstract: Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.

Cited by 14 publications

References 13 publications

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine

Three-Dimensional Avian Hematopoietic Stem Cell Cultures as a Model for Studying Disease Pathogenesis

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