A three-dimensional DNA walking machine for the ultrasensitive dual-modal detection of miRNA using a fluorometer and personal glucose meter

Wu, Tingting; Cao, Yu; Yang, Yuemeng; Zhang, Xiaoyan; Wang, Shutao; Xu, Li-Ping; Zhang, Xueji

doi:10.1039/c9nr03588e

Cited by 45 publications

(24 citation statements)

References 50 publications

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“…The quantification limit was found to be 5 pM when using two signaling probes that promote the labeling of each DNA duplex with two molecules of ALP. It should be pointed out that this is a value not estimated but experimentally determined, which favorably competes with those of previous works [ 14 , 28 , 29 ]. Lower values have also been reported [ 26 , 30 , 31 , 32 ] but the associated analytical methods entail numerous reagents and steps as well as longer times.…”

Section: Discussionsupporting

confidence: 85%

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Abardía-Serrano

Miranda‐Castro

de‐los‐Santos‐Álvarez

et al. 2020

Sensors

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A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

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Section: Discussionsupporting

confidence: 85%

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Abardía-Serrano

Miranda‐Castro

de‐los‐Santos‐Álvarez

et al. 2020

Sensors

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“…Propelled by strand displacement reaction, DNA tracks underwent structural change, and significant fluorescence enhancement was achieved for target miRNA sensing. 14 trigger surface programmatic chain reaction for electrochemiluminescence analysis. 15 Yan's group fabricated a tripedal walker using streptavidin as the inert "body" and three deoxyribozymes as three "legs".…”

mentioning

confidence: 99%

“…For example, Wu et al employed hairpin-structured DNA tracks on magnetic beads and target miRNA as walker strand. Propelled by strand displacement reaction, DNA tracks underwent structural change, and significant fluorescence enhancement was achieved for target miRNA sensing . Ge et al designed a bipedal walker constructed by target protein and two DNA aptamers, which was then used to trigger surface programmatic chain reaction for electrochemiluminescence analysis .…”

mentioning

confidence: 99%

Gold Nanoparticles-Based Multipedal DNA Walker for Ratiometric Detection of Circulating Tumor Cell

Miao

Tang

2019

Anal. Chem.

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Sensitive and accurate quantification of circulating tumor cell (CTC) can provide new insights for early diagnosis and prognosis of cancers. Herein, we have developed a multipedal DNA walker for ultrasensitive detection of CTC for the first time. Generally, a number of walker strands are simply modified on gold nanoparticle (AuNPs). The integrated aptamer sequence can specially interact with the transmembrane receptor protein of CTC and facilitate the enrichment of AuNPs on the surface of cells. After a low speed centrifugation, the complex of CTC and AuNPs could be precipitated and the supernate represents decreased UV–vis absorbance response of AuNPs. On the other hand, since multiple walker strands are modified on a single AuNP, hybridization with several tracks on the electrode occurs simultaneously for the following nicking endonuclease-catalyzed cleaving. Experimental results verify that the rate of multipedal walking is much faster. In addition, TCEP-mediated electrochemical amplification is employed to further enhance the electrochemical signal. By comparing the variations of electrochemical and UV–vis absorbance responses, ultrahigh sensitivity for CTC assay is achieved. The limit of detection is down to 1 cell/mL. The results of selectivity confirmation and blood sample test are also satisfactory. This AuNPs-based multipedal DNA walker offers a speedy analysis of CTC and shows great potential use for early clinical diagnosis and treatment of cancers.

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“…1d) of the devices reviewed in this work (Tables 3 and 4) utilize ECM transduction, a clear majority. Consider the success of the PGM as an example of this fact (153,(190)(191)(192). ECM nanobiosensors are those that quantify a bioanalyte via measurement of an electrical signal occurring after recognition of that target with an MIP.…”

Section: Electrochemical (Ecm) Transductionmentioning

confidence: 99%

Point-of-Care Diagnostics: Molecularly Imprinted Polymers and Nanomaterials for Enhanced Biosensor Selectivity and Transduction

Denmark

Mohapatra

2020

The EuroBiotech Journal

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Significant healthcare disparities resulting from personal wealth, circumstances of birth, education level, and more are internationally prevalent. As such, advances in biomedical science overwhelmingly benefit a minority of the global population. Point-of-Care Testing (POCT) can contribute to societal equilibrium by making medical diagnostics affordable, convenient, and fast. Unfortunately, conventional POCT appears stagnant in terms of achieving significant advances. This is attributed to the high cost and instability associated with conventional biorecognition: primarily antibodies, but nucleic acids, cells, enzymes, and aptamers have also been used. Instead, state-of-the-art biosensor researchers are increasingly leveraging molecularly imprinted polymers (MIPs) for their high selectivity, excellent stability, and amenability to a variety of physical and chemical manipulations. Besides the elimination of conventional bioreceptors, the incorporation of nanomaterials has further improved the sensitivity of biosensors. Herein, modern nanobiosensors employing MIPs for selectivity and nanomaterials for improved transduction are systematically reviewed. First, a brief synopsis of fabrication and wide-spread challenges with selectivity demonstration are presented. Afterward, the discussion turns to an analysis of relevant case studies published in the last five years. The analysis is given through two lenses: MIP-based biosensors employing specific nanomaterials and those adopting particular transduction strategies. Finally, conclusions are presented along with a look to the future through recommendations for advancing the field. It is hoped that this work will accelerate successful efforts in the field, orient new researchers, and contribute to equitable health care for all.

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A three-dimensional DNA walking machine for the ultrasensitive dual-modal detection of miRNA using a fluorometer and personal glucose meter

Abstract: A three-dimensional DNA walking machine for the dual-modal detection of miRNA-21 using a fluorometer and personal glucose meter.

Cited by 45 publications

References 50 publications

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Gold Nanoparticles-Based Multipedal DNA Walker for Ratiometric Detection of Circulating Tumor Cell

Point-of-Care Diagnostics: Molecularly Imprinted Polymers and Nanomaterials for Enhanced Biosensor Selectivity and Transduction

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