A thermoreversible hydrogel as a biosynthetic bandage for corneal wound repair

Pratoomsoot, Chayanin; Tanioka, Hidetoshi; Hori, Keiko; Kawasaki, Satoshi; Kinoshita, Shigeru; Tighe, Patrick J.; Dua, Harminder S; Shakesheff, Kevin M.; Rose, Felicity R. A. J.

doi:10.1016/j.biomaterials.2007.09.031

Cited by 88 publications

(71 citation statements)

References 30 publications

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“…Synthesis of PLGA-PEG-PLGA Triblock Copolymers Using the Classic Method Triblock copolymers were synthesized using a ring-opening method (37) with minor modifications (36). PEG1500 (30 %, w/w) was loaded into a stainless steel reactor and stirred at 150°C under vacuum for 2 h. Next, D,L-lactide and glycolide were loaded into the reactor at a molar ratio of either 3:1 or 5:1.…”

Section: Synthesis and Purification Of Plga-peg-plga Triblock Copolymersmentioning

confidence: 99%

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Preparation and Investigation of Sustained Drug Delivery Systems Using an Injectable, Thermosensitive, In Situ Forming Hydrogel Composed of PLGA–PEG–PLGA

et al. 2012

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Abstract. In situ gelling systems are very attractive for pharmaceutical applications due to their biodegradability and simple manufacturing processes. The synthesis and characterization of thermosensitive poly(D,L-lactic-co-glycolic acid) (PLGA)-polyethylene glycol (PEG)-PLGA triblock copolymers as in situ gelling matrices were investigated in this study as a drug delivery system. Ring-opening polymerization using microwave irradiation was utilized as a novel technique, and the results were compared with those using a conventional method of polymerization. The phase transition temperature and the critical micelle concentration (CMC) of the copolymer solutions were determined by differential scanning calorimetry and spectrophotometry, respectively. The size of the micelles was determined with a light scattering method. In vitro drug release studies were carried out using naltrexone hydrochloride and vitamin B12 as model drugs. The rate and yield of the copolymerization process via microwave irradiation were higher than those of the conventional method. The copolymer structure and concentration played critical roles in controlling the sol-gel transition temperature, the CMC, and the size of the nanomicelles in the copolymer solutions. The rate of drug release could be modulated by the molecular weight of the drugs, the concentration of the copolymers, and their structures in the formulations. The amount of release versus time followed zero-order release kinetics for vitamin B12 over 25 days, in contrast to the Higuchi modeling for naltrexone hydrochloride over a period of 17 days. In conclusion, PLGA-PEG1500-PLGA with a lactide-to-glycolide ratio of 5:1 is an ideal system for the long-acting, controlled release of naltrexone hydrochloride and vitamin B12.

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Section: Synthesis and Purification Of Plga-peg-plga Triblock Copolymersmentioning

confidence: 99%

“…Gel formulations of this copolymer have been shown to provide a unique controlled release of paclitaxel (Oncogel®) in tumors for approximately 50 days with a minimal distribution in other organs (35). PLGA-PEG-PLGA is also used as a biosynthetic bandage for corneal wound repair (36).…”

Section: Introductionmentioning

confidence: 99%

Preparation and Investigation of Sustained Drug Delivery Systems Using an Injectable, Thermosensitive, In Situ Forming Hydrogel Composed of PLGA–PEG–PLGA

et al. 2012

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“…Therefore, in more advanced applications it would be desirable to have a range of properties which could be modifiable. This has prompted a search for development of new artificial membranes [13][14][15]. Nonetheless, the properties of AM become important to use as model to guide the development of new synthetic substitutes.…”

Section: Introductionmentioning

confidence: 99%

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

et al. 2010

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“…[20][21][22][23][24][25] A biodegradable, amphiphilic triblock copolymer, for example, poly(dl-lactide-co-glycolide-bethylene glycol-b-dl-lactide-co-glycolide) (PLGA-PEG-PLGA), was proven to be a potential bandage by its gelling profile and biocompatibility properties. 26 Anumolu and colleagues demonstrated that poly(ethyleneglycol) (PEG)-based doxycycline hydrogel accelerated corneal wound healing in half-mustard and nitrogen mustard-exposed rabbit corneas in organ culture. 27 Further, the application of these materials obtained from nature to promote corneal injury was also evaluated.…”

Section: Introductionmentioning

confidence: 99%

Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium

Chen¹,

Lee²,

Chen³

et al. 2012

IJN

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Abstract:The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, mSC has been produced successfully and its chemical properties confirmed, and physical and rheological properties characterized. An in vitro cell proliferation study has revealed that at the concentrations of 200, 300, and 400 µg/mL, mSC nanogel significantly increased Statens Seruminstitut rabbit corneal (SIRC) cell proliferation after 24 hours of incubation. In cell migration assay, migration of SIRC cell to wound closure was observed after 24 hours of incubation with the addition of 200 µg/mL mSC of nanogel. In an animal study, acceleration of corneal wound healing was probably due to the inhibition of proteolysis. In conclusion, the findings of this study substantiate the potential application of sacchachitin in the form of mSC nanogel for the treatment of superficial corneal injuries.

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A thermoreversible hydrogel as a biosynthetic bandage for corneal wound repair

Cited by 88 publications

References 30 publications

Preparation and Investigation of Sustained Drug Delivery Systems Using an Injectable, Thermosensitive, In Situ Forming Hydrogel Composed of PLGA–PEG–PLGA

Preparation and Investigation of Sustained Drug Delivery Systems Using an Injectable, Thermosensitive, In Situ Forming Hydrogel Composed of PLGA–PEG–PLGA

Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction

Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium

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